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Small non-coding RNAs : methods and protocols / edited by Mathieu Rederstorff.

Contributor(s): Material type: TextTextSeries: Methods in molecular biology (Clifton, N.J.) ; v. 2300.Publisher: New York, NY : Humana Press, 2021Edition: Second editionDescription: 1 online resource (x, 270 pages) : illustrations (some color)Content type:
  • text
Media type:
  • computer
Carrier type:
  • online resource
ISBN:
  • 9781071613863
  • 1071613863
Subject(s): Genre/Form: DDC classification:
  • 572.8/8 23
LOC classification:
  • QP623
Online resources:
Contents:
Regulatory Non-coding RNAs: An Overview -- RNA Precipitation -- Quantitative and Qualitative Assessment of Small RNA Preparations -- Non-coding RNA Silencing in Mammalian Cells by Antisense LNA GapmeRs Transfection -- Northern Blot Detection of Tiny RNAs -- Stem-loop qRT-PCR Based Quantification of miRNAs -- Detection and Labeling of Small Non-coding RNAs by Splinted Ligation -- Fluorescence In Situ Hybridization Of Small Non-coding RNAs -- Using native RIP, UV-CLIP or fCLIP to Address Protein-RNA Interactions In Vivo -- Purification of RiboNucleoProtein Particles by MS2-MBP Affinity Chromatography -- Study of genome-wide occupancy of long non-coding RNAs using Chromatin Isolation by RNA Purification (ChIRP) -- Gene Reporter Assays to Study miRNA Function -- CRISPR/Cas9 System to Knock-Down microRNA In Vitro and In Vivo -- Microarray Analysis of Whole-Transcriptome RNAs including Non-Coding RNAs -- Isolation, Extraction, and Deep-Sequencing Analysis Of Extracellular RNAs (exRNAs) from Human Plasma -- Analysis of the RNA and protein complexome by Grad-seq -- uIVC-Seq: A Method for Ultrahigh-throughput Development and Functional Characterization of Small RNAs -- RNA Secondary Structure Study by Chemical Probing Methods using DMS and CMCT -- Application of NMR Spectroscopy to Determine the 3D structure of Small Non-coding RNAs.
Summary: This second edition provides new and updated chapters detailing methods tackling all aspects of small non-coding RNAs biology. Chapters guide readers through customized dedicated protocols and technologies that will help decipher the numerous functions of small non-coding RNAs. Written in the highly successful Methods of Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and key tips on troubleshooting and avoiding known pitfalls. Instructive and practical, Small Non-Coding RNAs: Methods and Protocols, Second Edition aims to be a valuable help and of great interest to a large number of researchers working in the field of RNA or willing to develop RNA studies.
Holdings
Item type Current library Collection Call number Status Date due Barcode Item holds
eBook eBook e-Library eBook Protocols Available
Total holds: 0

Regulatory Non-coding RNAs: An Overview -- RNA Precipitation -- Quantitative and Qualitative Assessment of Small RNA Preparations -- Non-coding RNA Silencing in Mammalian Cells by Antisense LNA GapmeRs Transfection -- Northern Blot Detection of Tiny RNAs -- Stem-loop qRT-PCR Based Quantification of miRNAs -- Detection and Labeling of Small Non-coding RNAs by Splinted Ligation -- Fluorescence In Situ Hybridization Of Small Non-coding RNAs -- Using native RIP, UV-CLIP or fCLIP to Address Protein-RNA Interactions In Vivo -- Purification of RiboNucleoProtein Particles by MS2-MBP Affinity Chromatography -- Study of genome-wide occupancy of long non-coding RNAs using Chromatin Isolation by RNA Purification (ChIRP) -- Gene Reporter Assays to Study miRNA Function -- CRISPR/Cas9 System to Knock-Down microRNA In Vitro and In Vivo -- Microarray Analysis of Whole-Transcriptome RNAs including Non-Coding RNAs -- Isolation, Extraction, and Deep-Sequencing Analysis Of Extracellular RNAs (exRNAs) from Human Plasma -- Analysis of the RNA and protein complexome by Grad-seq -- uIVC-Seq: A Method for Ultrahigh-throughput Development and Functional Characterization of Small RNAs -- RNA Secondary Structure Study by Chemical Probing Methods using DMS and CMCT -- Application of NMR Spectroscopy to Determine the 3D structure of Small Non-coding RNAs.

Includes bibliographical references and index.

This second edition provides new and updated chapters detailing methods tackling all aspects of small non-coding RNAs biology. Chapters guide readers through customized dedicated protocols and technologies that will help decipher the numerous functions of small non-coding RNAs. Written in the highly successful Methods of Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and key tips on troubleshooting and avoiding known pitfalls. Instructive and practical, Small Non-Coding RNAs: Methods and Protocols, Second Edition aims to be a valuable help and of great interest to a large number of researchers working in the field of RNA or willing to develop RNA studies.

Online resource; title from PDF title page (SpringerLink, viewed April 14, 2021).

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