Gene therapy methods / edited by M. Ian Phillips.

Includes bibliographical references and indexes.

Chapter 1. Gene Therapy for Hypertension: The Preclinical Data; Chapter 2. Oligonucleotide-Mediated Site-Directed Gene Repair; Chapter 3. Stabilized Plasmid-Lipid Particles: A Systemic Gene Therapy Vector; Chapter 4. Enhancing Direct in Vivo Transfection with Nuclease Inhibitors and Pulsed Electrical Fields; Chapter 5. Hydrodynamics-Based Transfection: Simple and Efficient Method for Introducing and Expressing Transgenes in Animals by Intravenous Injection of DNAChapter 6. Bioplex Technology: Novel Synthetic Gene Delivery System Based on Peptides Anchored to Nucleic Acids; Chapter 7. Surgical Procedures for Intravascular Delivery of Plasmid DNA to Organs; Chapter 8. Direct Gene Transfer into Mouse Heart; Section II: Adenovirus; Chapter 9. Myoblast-Mediated Gene Transfer for Therapeutic Angiogenesis; Chapter 10. Use of Phage Display to Identify Novel Peptides for Targeted Gene Therapy; Chapter 11. Helper-Dependent Adenoviral VectorsChapter 12. Gene Transfer Methods for Transplantation; Chapter 13. Generation and Growth of Gutted Adenoviral Vectors; Chapter 14. Adenovirus-Mediated Gene Transfer for Cardiovascular and Renal Diseases; Chapter 15. Gene Transfer to Blood Vessels Using Adenoviral Vectors; Chapter 16. Rearrangements in Adenoviral Genomes Mediated by Inverted Repeats; Chapter 17. High-Capacity, Helper-Dependent, "Gutless" AdenoviralVectors for Gene Transfer into Brain; Chapter 18. Gene Therapy Methods in Cardiovascular Diseases; Section III: Adeno-Associated Virus; Chapter 19. Gene Delivery to Cardiac MuscleChapter 20. Recombinant AAV-Mediated Gene Delivery Using Dual Vector Heterodimerization; Chapter 21. Designing and Characterizing Hammerhead Ribozymes for Use in AAV Vector-Mediated Retinal Gene Therapies; Chapter 22. Adeno-Associated Viral Vector-Mediated Gene Therapy of Ischemia-Induced Neuronal Death; Chapter 23. Recombinant Adeno-Associated Viral Vector Production Using Stable Packaging and Producer Cell Lines; Chapter 24. Streamlined Large-Scale Production of Recombinant Adeno-Associated Virus (rAAV) Vectors; Section IV: Lentivirus; Chapter 25. Gene Transfer to the Brain Using Feline Immunodeficiency Virus-Based Lentivirus VectorsChapter 26. Generation of HIV-1 Derived Lentiviral Vectors; Chapter 27. Design, Production, Safety, Evaluation, and Clinical Applications of Nonprimate Lentiviral Vectors; Chapter 28. Gene Transfer to Airway Epithelia Using Feline Immunodeficiency Virus-Based Lentivirus Vectors; Chapter 29. Transduction of a Gene Expression Cassette Using Advanced Generation Lentiviral Vectors; Chapter 30. Construction, Purification, and Characterization of Adenovirus Vectors Expressing Apoptosis- Inducing Transgenes

Print version record.

This volume in the prestigious Methods in Enzymology series discusses methods currently used in preclinical and clinical gene therapy. Subjects covered in this book, such as the use of adeno-associated virus delivery for treatment of Parkinson's disease, are topical and are presented in the methods-oriented style popularized by this series.