Mitochondrial function. Part B : mitochondrial protein kinases, protein phosphatases and mitochondrial diseases / edited by William S. Allison and Anne N. Murphy.

Includes bibliographical references and indexes.

Print version record.

The second of two new volumes covering mitochondria, this volume presents methods that have been developed to assess the number and function of nuclear-encoded proteins in the mitochndrion. These diverse chapters focus on regulation of mitochondrial function and mitochondrial diseases, including a section emphasizing the mitochondrial defects associated with type-2 diabetes. The critically acclaimed laboratory standard for 40 years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. With more than 400 volumes published, each Methods in Enzymology volume presents material that is relevant in today's labs -- truly an essential publication for researchers in all fields of life sciences. New methods focusing on the examination of normal and abnormal mitochondrial function are presented in an easy-to-follow format by the researchers who developed them Along with companion volume covering mitochondrial electron transport chain complexes, iron-sulfur proteins and reactive oxygen species, provides a comprehensive overview of modern techniques in the study of mitochondrial malfunction Provides a "one-stop shop" for tried and tested essential techniques, eliminating the need to wade through untested or unreliable methods

Front Cover; Mitochondrial Function, Part B: Mitochondrial Protein Kinases, Protein Phosphatases and Mitochondrial Diseases; Copyright Page; Contents; Contributors; Preface; Methods in Enzymology; Section 1: Defining the Mitochondrial Proteome, Including Phosphorylated, Acetylated, and Palmitoylated Proteins; Chapter 1: The Mitochondrial Proteome Database: MitoP2; 1. Introduction; 2. Yeast, Human, and Mouse Proteome Information; 3. Mitochondrial Reference Set, Candidates, and SVM Prediction Score; 4. Integrated Genome-Wide Approaches; 6. Output Lists and Practical Examples; References

Chapter 2: Predicting Proteomes of Mitochondria and Related Organelles from Genomic and Expressed Sequence Tag Data1. Introduction; 3. Screening Large Datasets for Mitochondrial Proteins; 4. Detailed Analysis; 5. Comparison of Methods; Acknowledgments; References; Chapter 3: Proteome Characterization of Mouse Brain Mitochondria Using Electrospray Ionization Tandem Mass Spectrometry; 1. Introduction; 3. Selective Proteome Enrichment by Capillary Isotachophoresis (CITP)-Based Separations; 4. Analysis of Mouse Brain Mitochondria Using the CITP-Based Proteome Platform

5. Proteome Characterization of Mouse Brain MitochondriaReferences; Chapter 4: 32P Labeling of Protein Phoshorylation and Metabolite Association in the Mitochondria Matrix; 1. Introduction; 2. Methods; 3. Results and Discussion; References; Chapter 5: Selective Enrichment in Phosphopeptides for the Identification of Phosphorylated Mitochondrial Proteins; 1. Introduction; 2. Off-Line Phosphopeptide Enrichment Methods; 4. Mass Spectrometry-Based Methods; 5. Quantitative Phosphoproteomics; Chapter 6: Post-translational Modifications of Mitochondrial Outer Membrane Proteins; 1. Introduction

4. Focused Proteomics Using Immunological Approaches6. Conclusion; References; Chapter 7: Analysis of Tyrosine-Phosphorylated Proteins in Rat Brain Mitochondria; 1. Introduction; 2. Identification of Src Tyrosine Kinase Substrates in Rat Brain Mitochondria; 3. Mass Spectrometric Analysis of Tyrosine-Phosphorylated Peptides in Mitochondria; 4. Bioinformatic Tools to Analyze the Potential Role of Tyrosine Phosphorylation; Acknowledgment; References; Chapter 8: Acetylation of Mitochondrial Proteins; 1. Introduction; 3. SIRT3 Enzymatic Deacetylation Assay

4. Detection of Acetylated Proteins in MitochondriaReferences; Chapter 9: Non-radioactive Detection of Palmitoylated Mitochondrial Proteins Using an Azido-Palmitate Analogue; 2. Synthesis of 12-Azidododecanoate and 14-Azidotetradecanoate; 3. Synthesis of Tagged Phosphine Probes; 5. Production of 14-Azidotetradecanoyl-CoA Derivatives; 7. Isolation of Intact Mitochondria from Livers of Sprague-Dawley Rats; 8. Chromatography of Soluble Mitochondrial Proteins; 9. Labeling of Anion Exchange Eluates with 14-Azidotetradecanoyl-CoA and Phosphine-Biotin