Amazon cover image
Image from Amazon.com

Aqueous two-phase systems / edited by Harry Walter, Göte Johansson.

Contributor(s): Material type: TextTextSeries: Methods in enzymology ; v. 228.Publication details: San Diego : Academic Press, ©1994.Description: 1 online resource (xxx, 725 pages) : illustrationsContent type:
  • text
Media type:
  • computer
Carrier type:
  • online resource
ISBN:
  • 0121821293
  • 9780121821296
Subject(s): Genre/Form: Additional physical formats: Print version:: Aqueous two-phase systems.DDC classification:
  • 660.6 20
LOC classification:
  • QP601 .M49 vol. 228
  • QH585.5.C43
NLM classification:
  • W1
  • QH 585.5C43
Online resources:
Contents:
Section I. General methodology and apparatus -- Section II. Partitioning and affinity partitioning of macromolecules -- A. Proteins -- B. Nucleic acids -- Section III. Studies on protein interactions, molecular structure, charge, hydrophobicity, and conformational changes -- Section IV. Partitioning and affinity partitioning of particulates -- A. Cells: Separation and fractionation of cell populations and obtaining information on their surface properties -- Affinity partitioning -- B. Organelles: Separation and subfractionation -- C. Membranes: Separation and subfractionation -- Affinity partitioning -- Membrane domain analysis -- Section V. Aqueous phase separations in biological systems -- Section VI. Aqueous two-phase systems in large-scale processes: Biotechnology -- A. Extractive bioconversions -- B. Proteins: Downstream processing -- C. Design of proteins for enhanced extraction -- D. Other applications of aqueous phases in biotechnology.
Summary: This volume of Methods in Enzymology represents the sole collection of separation methods based on aqueous two-phase systems. It includes procedures for the isolation of proteins, especially enzymes, nucleic acids, cell membranes, and organelles, as well as for the separation and study of cells. Key Features * Use of affinity partitioning for enzyme purification * Separation and study of cells * Isolation of plasma membranes * Large scale biotechnical use.
Holdings
Item type Current library Collection Call number Status Date due Barcode Item holds
eBook eBook e-Library eBook Elsevier Available
Total holds: 0

Includes bibliographical references and indexes.

Section I. General methodology and apparatus -- Section II. Partitioning and affinity partitioning of macromolecules -- A. Proteins -- B. Nucleic acids -- Section III. Studies on protein interactions, molecular structure, charge, hydrophobicity, and conformational changes -- Section IV. Partitioning and affinity partitioning of particulates -- A. Cells: Separation and fractionation of cell populations and obtaining information on their surface properties -- Affinity partitioning -- B. Organelles: Separation and subfractionation -- C. Membranes: Separation and subfractionation -- Affinity partitioning -- Membrane domain analysis -- Section V. Aqueous phase separations in biological systems -- Section VI. Aqueous two-phase systems in large-scale processes: Biotechnology -- A. Extractive bioconversions -- B. Proteins: Downstream processing -- C. Design of proteins for enhanced extraction -- D. Other applications of aqueous phases in biotechnology.

Print version record.

This volume of Methods in Enzymology represents the sole collection of separation methods based on aqueous two-phase systems. It includes procedures for the isolation of proteins, especially enzymes, nucleic acids, cell membranes, and organelles, as well as for the separation and study of cells. Key Features * Use of affinity partitioning for enzyme purification * Separation and study of cells * Isolation of plasma membranes * Large scale biotechnical use.

English.

Powered by Koha