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Lipid modifications of proteins / edited by Patrick J. Casey, Janice E. Buss.

Contributor(s): Material type: TextTextSeries: Methods in enzymology ; v. 250.Publication details: San Diego : Academic Press, ©1995.Description: 1 online resource (xxxiii, 754 pages) : illustrationsContent type:
  • text
Media type:
  • computer
Carrier type:
  • online resource
ISBN:
  • 012182151X
  • 9780121821517
Subject(s): Genre/Form: Additional physical formats: Print version:: Lipid modifications of proteins.DDC classification:
  • 574.19245 20
LOC classification:
  • QP601 .M49 vol. 250
  • QP551
NLM classification:
  • W1
  • QU 85
Other classification:
  • 35.75
  • WC 4355
  • WD 5050
  • CHE 820f
  • CHE 840f
Online resources:
Contents:
Section 1. Prenylation -- section 2. Prenylation-dependent processing -- section 3. Palmitoylaton -- section 4. Myristoylation -- section 5. Glycosylphosphatidylinositol-anchored proteins -- section 6. Other modifications.
Action note:
  • digitized 2011 HathiTrust Digital Library committed to preserve
Summary: General Description of the Volume: This volume presents the best techniques and strategies for the study of lipid modified proteins, with particular emphasis on methods which evaluate the functional significance or biological impact of lipid modification. Included are the biological methods for the study of function (yeast genetics; cloning strategies; mutational analysis; expression systems), biochemical methods for the study and purification of enzymes or modified proteins (in vitro assays using peptide, native, or recombinant protein substrates; coupled in vitro cDNA transcription, translation/modification; bacolovirus expression; lipid analogs/inhibitors); physical methods for the identification of lipid groups (cleavage techniques; modification techniques; simple separations: TLC, GC, HPLC, ES/MS, tandem MS). General Description of the Series: The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today--truly an essential publication for researchers in all fields of life sciences.
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Includes bibliographical references and indexes.

Print version record.

General Description of the Volume: This volume presents the best techniques and strategies for the study of lipid modified proteins, with particular emphasis on methods which evaluate the functional significance or biological impact of lipid modification. Included are the biological methods for the study of function (yeast genetics; cloning strategies; mutational analysis; expression systems), biochemical methods for the study and purification of enzymes or modified proteins (in vitro assays using peptide, native, or recombinant protein substrates; coupled in vitro cDNA transcription, translation/modification; bacolovirus expression; lipid analogs/inhibitors); physical methods for the identification of lipid groups (cleavage techniques; modification techniques; simple separations: TLC, GC, HPLC, ES/MS, tandem MS). General Description of the Series: The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today--truly an essential publication for researchers in all fields of life sciences.

Section 1. Prenylation -- section 2. Prenylation-dependent processing -- section 3. Palmitoylaton -- section 4. Myristoylation -- section 5. Glycosylphosphatidylinositol-anchored proteins -- section 6. Other modifications.

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