Electron Paramagnetic Resonance Investigations of Biological Systems by Using Spin Labels, Spin Probes, and Intrinsic Metal Ions. Part A / edited by Peter Z. Qin, Kurt Warncke.

Text in English.

Includes bibliographical references and indexes.

Print version record.

Title page; Table of Contents; Copyright; Contributors; Preface; Section I: EPR Methodology and Instrumentation; Chapter One: Rapid-Scan EPR of Nitroxide Spin Labels and Semiquinones; Abstract; 1 Introduction; 2 Rapid-Scan Method; 3 Why Rapid Scan Improves S/N; 4 Other Advantages of Rapid Scan; 5 Hardware Requirements; 6 Parameter Selection; 7 Examples; 8 Wider Scans; 9 Future Applications; Acknowledgments; Chapter Two: Fourier Deconvolution Methods for Resolution Enhancement in Continuous-Wave EPR Spectroscopy; Abstract; 1 Introduction; 2 Historical Perspective; 3 Overlapping Signals

4 Fourier Filtering5 Overview of Method; 6 Practical Examples; 7 Limitations of the Method; 8 Summary; Acknowledgment; Supplementary materials; Chapter Three: Multifrequency Pulsed EPR and the Characterization of Molecular Dynamics; Abstract; 1 Introduction; 2 Contributions to Electron Spin-Lattice Relaxation and Dependence of Relaxation on Motions in Fluid Solution; 3 Measurement of Spin-Lattice Relaxation, T1; 4 Examples of Motions Elucidated by Measuring T1 as a Function of Frequency; 5 Conclusion; Acknowledgments

Chapter Four: Resolution and Characterization of Chemical Steps in Enzyme Catalytic Sequences by Using Low-Temperature and Time-Resolved, Full-Spectrum EPR Spectroscopy in Fluid Cryosolvent and Frozen Solution SystemsAbstract; 1 Introduction; 2 Low-Temperature Fluid Cryosolvent System; 3 Low-Temperature Frozen Solution System; 4 Analysis and Interpretations: General Cases and Guidelines; 5 Conclusions; Acknowledgments; Chapter Five: 230/115 GHz Electron Paramagnetic Resonance/Double Electron-Electron Resonance Spectroscopy; Abstract; 1 Introduction; 2 HF EPR/DEER Spectrometer

3 Examples of HF EPR: Paramagnetic Impurities in Diamonds Studied by 230 GHz EPR/DEER Spectroscopy4 Summary; Acknowledgments; Appendix; Section II: EPR Spectral Simulation and Software; Chapter Six: CW-EPR Spectral Simulations: Solid State; Abstract; 1 Introduction; 2 Spins and SH; 3 Dynamic Regime; 4 Levels of Theory; 5 Orientational Order and Disorder; 6 Structural Order and Disorder; 7 Other Line Broadenings; 8 Experimental Effects; 9 Fitting; 10 Conclusions; Chapter Seven: CW-EPR Spectral Simulations: Slow-Motion Regime; Abstract; 1 Introduction; 2 Magnetic Parameters

3 Methods of Modeling Slow Motion4 Outlook; Acknowledgment; Chapter Eight: Quantitative Interpretation of Multifrequency Multimode EPR Spectra of Metal Containing Proteins, Enzymes, and Biomimetic Complexes; Abstract; 1 Introduction; 2 Basic EPR Theory; 3 g-Factor Anisotropy; 4 Hyperfine Structure; 5 Spin Hamiltonian; 6 Basic EPR Instrumentation; 7 Simulation of Powder Spectra; 8 Quantitative Aspects; 9 Applications; 10 Conclusion; Acknowledgments; Section III: EPR Studies of Metal-Containing Proteinsand Metal Cofactors

Electron Paramagnetic Resonance Investigations of Biological Systems by Using Spin Labels, Spin Probes, and Intrinsic Metal Ions Part A & B, are the latest volumes in the Methods in Enzymology series, continuing the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers research methods centered on the use of Electron Paramagnetic Resonance (EPR) techniques to study biological structure and function. Timely contribution that describes a rapidly changing field Leading researchers in the field Broad coverage: Instrumentation, basic theory, data analysis, and applications.