Chemical and biochemical approaches for the study of anesthetic function. Part B / edited by Roderic G. Eckenhoff, Ivan J. Dmochowski.

Online resource; title from PDF title page (EBSCO, viewed April 24, 2018).

Includes bibliographical references.

Intro; Title page; Table of Contents; Copyright; Contributors; Preface; Section I: Structural Approaches; Chapter One: Structural Analysis of Anesthetics in Complex with Soluble Proteins; Abstract; 1 Introduction; 2 Crystallographic Issues Relevant to Analysis of Anesthetic Binding; 3 Crystallizing Apoferritin with Isoflurane and Propofol; 4 Protocol; 5 Analysis of the Structure; 6 Summary; Acknowledgment; Chapter Two: X-Ray Crystallographic Studies for Revealing Binding Sites of General Anesthetics in Pentameric Ligand-Gated Ion Channels; Abstract; 1 Introduction.

2 Production of Monodisperse pLGICs with High Purity3 Crystallization of pLGICs in the Absence and Presence of General Anesthetics; 4 Functional Validation of the Bound Anesthetics; 5 Case Studies; Acknowledgments; Chapter Three: Solution NMR Studies of Anesthetic Interactions with Ion Channels; Abstract; 1 Introduction; 2 Preparation of Ion Channels for Solution NMR; 3 NMR Methods to Identify Anesthetics Binding Sites and Detect Anesthetic-Induced Dynamics Changes; 4 Case Studies; Acknowledgments.

Chapter Four: Time-Resolved Neutron Interferometry and the Mechanism of Electromechanical Coupling in Voltage-Gated Ion ChannelsAbstract; 1 Introduction; 2 Methods; 3 Results; 4 Discussion; 5 Conclusions and Future Prospects; Acknowledgments; Section II: Evolving Biophysical Technologies; Chapter Five: Fluorescent Anesthetics; Abstract; 1 Introduction; 2 Value of 1-AMA for Studying General Anesthetic Mechanisms; 3 Value of 1-AZA for Studying General Anesthetic Mechanisms; 4 Experiments; Chapter Six: Investigation of Anesthetic-Protein Interactions by a Thermodynamic Approach; Abstract.

1 Introduction2 ITC Measurements; 3 Conclusion; Chapter Seven: Electron Spin Resonance (EPR) in Drosophila and General Anesthesia; Abstract; 1 Introduction; 2 Electron Spin Resonance Primer; 3 Quantitation; 4 Sample Preparation; 5 Measurements; 6 Origin of the Spin Signal in Drosophila; 7 Conclusions; Chapter Eight: Giant Plasma Membrane Vesicles: An Experimental Tool for Probing the Effects of Drugs and Other Conditions on Membrane Domain Stability; Abstract; 1 Introduction; 2 GPMV Formation; 3 Measuring GPMV Miscibility Transition Temperatures; 4 Summary and Conclusion; Acknowledgments.

Section III: In vivo Technologies Established and Evolving; Chapter Nine: Anesthetic Drug Discovery and Development: A Case Study of Novel Etomidate Analogs; Abstract; 1 Introduction; 2 Etomidate Analog Design Strategies; 3 Testing Novel Etomidate Analogs for Hypnotic Activity Using Loss of Righting Reflexes Assays; 4 Testing Novel Etomidate Analogs for Adrenocortical Inhibitory Activity; 5 Summary and Conclusion; Acknowledgments; Chapter Ten: Optoanesthesia: Use of Anesthetic Photolabels In Vivo; Abstract; 1 Introduction; 2 Anesthetic Photolabel Availability.

Chemical and Biochemical Approaches for the Study of Anesthetic Function, Part B, Volume 603, presents a coherent description of the campaign towards understanding anesthesia. It includes a variety of highly debated topics, including sections on computational approaches, best practices for simulating ligand-gated ion channels interacting with general anesthetics, computational approaches for studying voltage-gated ion channels modulation by general anesthetics, anesthetic parameterization, the kinetic modeling of electrophysiology data, evolving biophysical technologies, fluorescent anesthetics, lipids, membranes and pressure reversal, in vivo technologies, and more. Helps readers understand the wide array of topics surrounding anesthesia Includes sections on Pharmacophore QSAR, QM, ONIOM, and the kinetic modeling of electrophysiology data Broaches genetics, model organisms and general genetic strategies.