Tumor immunology and immunotherapy : molecular methods / volume editor, Lorenzo Galluzzi, Nils-Petter Rudqvist.

Includes bibliographical references.

Tumor Immunology and Immunotherapy - Molecular Methods, Volume 629, the latest release in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Chapters in this release include Droplet digital PCR for measuring circulating tumor-derived DNA, Detection and quantification of cytosolic DNA, Methods to detect endogenous dsRNA induction and recognition, Quantification of eIF2alpha phosphorylation during immunogenic cell death, Assessment of annexin A1 release during immunogenic cell death, Luciferase-assisted detection of extracellular ATP in the course of ICD, The P2X7 receptor: structure and function, and much more. Contains the authority of authors who are leaders in their field Provides a comprehensive source on new methods and research in enzymology.

Preface. More than two decades of modern tumor immunology / Lorenzo Galluzzi, Nils-Petter Rudqvist -- Chapter One. Detection of circulating tumor DNA (ctDNA) by digital droplet polymerase chain reaction (dd-PCR) in liquid biopsies / Sara Valpione, Luca Campana -- Chapter Two. Detection and quantification of cytosolic DNA / Sheila Spada, Takahiro Yamazaki, Claire Vanpouille-Box -- Chapter Three. Methods to detect endogenous dsRNA induction and recognition / Ilias Ettayebi, Helen Loo Yau, Daniel D. De Carvalho -- Chapter Four. Quantification of eIF2alpha phosphorylation during immunogenic cell death / Lucillia Bezu, Alejandra Wu Chuang, Juliette Humeau, Guido Kroemer, Oliver Kepp -- Chapter Five. Assessment of annexin A1 release during immunogenic cell death / Elisa Elena Baracco, Adriana Petrazzuolo, Guido Kroemer -- Chapter Six. Luciferase-assisted detection of extracellular ATP and ATP metabolites during immunogenic death of cancer cells / George R. Dubyak -- Chapter Seven. Quinacrine-mediated detection of intracellular ATP / Sabrina Forveille, Juliette Humeau, Allan Sauvat, Lucillia Bezu, Guido Kroemer, Oliver Kepp -- Chapter Eight. Structure, function and techniques of investigation of the P2X7 receptor (P2X7R) in mammalian cells / Francesco Di Virgilio, Lin-Hua Jiang, Sébastien Roger, Simonetta Falzoni, Alba Clara Sarti, Valentina Vultaggio-Poma, Paola Chiozzi, Elena Adinolfi -- Chapter Nine. LEGENDplexTM: bead-assisted multiplex cytokine profiling by flow cytometry / Jason S. Lehmann, Priyanka Rughwani, Melissa Kolenovic, Shaoquan Ji, Binggang Sun -- Chapter Ten. Methods for measuring HMGB1 release during immunogenic cell death / Liwei Zhao, Peng Liu, Oliver Kepp, Guido Kroemer -- Chapter Eleven. Different measures of HMGB1 location in cancer immunology / Carolina Mendonça Gorgulho, Pranav Murthy, Lance Liotta, Virginia Espina, Michael T. Lotze -- Chapter Twelve. Reliable detection of indoleamine 2,3 dioxygenase-1 in murine cells and tissues / Sunil Thomas, Lisa Laury-Kleintop, George C. Prendergast

Chapter Thirteen. Quantification of IDO1 enzyme activity in normal and malignant tissues / Lijie Zhai, Erik Ladomersky, April Bell, Corey Dussold, Krislyn Cardoza, Jun Qian, Kristen L. Lauing, Derek A. Wainwright -- Chapter Fourteen. Enzyme activity of circulating CD73 in human serum / Silvana Morello, Roberta Turiello, Gabriele Madonna, Aldo Pinto, Paolo A. Ascierto, Mariaelena Capone -- Chapter Fifteen. Measurement of CD73 enzymatic activity using luminescence-based and colorimetric assays / Bertrand Allard, Isabelle Cousineau, Kathleen Spring, John Stagg -- Chapter Sixteen. Distinguishing perforin-mediated lysis and granzyme-dependent apoptosis / Jesse A. Rudd-Schmidt, Joseph A. Trapani, Ilia Voskoboinik -- Chapter Seventeen. Elucidating the role of an immunomodulatory protein in cancer: from protein expression to functional characterization / Leepakshi Khurana, Mei ElGindi, Pathricia V. Tilstam, Georgios Pantouris -- Chapter Eighteen. In vitro assay for the development of small molecule inhibitors targeting PD-1/PD-L1 / Wenjie Zhai, Xiuman Zhou, Jiangfeng Du, Yanfeng Gao -- Chapter Nineteen. Crystallographic approaches to study the interaction modes of PD-1- and CTLA-4-blocking antibodies / Norimichi Nomura, Yayoi Nomura, Yumi Sato, So Iwata -- Chapter Twenty. High-throughput discovery of cancer-targeting TCRs / Lukas Bunse, Edward W. Green, Michael Platten -- Chapter Twenty-One. TCR validation toward gene therapy for cancer / Edward W. Green, Lukas Bunse, Matthias Bozza, Khwab Sanghvi, Michael Platten -- Chapter Twenty-Two. Analysis of TCR [beta] CDR3 sequencing data for tracking anti-tumor immunity / Jiajia Zhang, Zhicheng Ji, Kellie N. Smith -- Chapter Twenty-Three. Quantitative analysis of the T cell receptor repertoire / Imran Uddin, Annemarie Woolston, Thomas Peacock, Kroopa Joshi, Mazlina Ismail, Tahel Ronel, Connor Husovsky, Benny Chain -- Chapter Twenty-Four. CRATES: a one-step assembly method for Class 2 CRISPR arrays / Chunyu Liao, Rebecca A. Slotkowski, Chase L. Beisel.