Single-molecule cellular biophysics / Mark C. Leake.

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Includes bibliographical references and index.

"Recent advances in single molecule science have presented a new branch of science: single molecule cellular biophysics, combining classical cell biology with cutting-edge single molecule biophysics. This textbook explains the essential elements of this new discipline, from the state-of-the-art single molecule techniques to real-world applications in unravelling the inner workings of the cell. Every effort has been made to ensure the text can be easily understood by students from both the physical and life sciences. Mathematical derivations are kept to a minimum whilst unnecessary biological terminology is avoided and text boxes provide readers from either background with additional information. 100 end-of-chapter exercises are divided into those aimed at physical sciences students, those aimed at life science students and those that can be tackled by students from both disciplines. The use of case studies and real research examples make this textbook indispensable for undergraduate students entering this exciting field"-- Provided by publisher.

Preface; Life, from the bottom up; References; 1 Once upon a (length and) time (scale); 1.1 Introduction; 1.2 There are already many informative `multi-molecule ́ methods; 1.2.1 Calorimetry; 1.2.2 Chromatography and dialysis; 1.2.3 Circular dichroism and optical rotation; 1.2.4 Electron microscopy; 1.2.5 Electrophysiology; 1.2.6 Fluorimetry; 1.2.7 Gel eletrophoresis; 1.2.8 Mass spectrometry; 1.2.9 NMR and ESR spectroscopy; 1.2.10 Optical interferometry; 1.2.11 Optical microscopy; 1.2.12 Radioactivity; 1.2.13 Spectrophotometry; 1.2.14 Sedimentation methods.

1.2.15 X-ray, neutron and electron diffraction1.3 American versus European coffee; 1.4 Scales of length, force, energy, time and concentration; 1.4.1 Length; 1.4.2 Force; 1.4.3 Energy; 1.4.4 Time; 1.4.5 Concentration; 1.5 Some basic thermodynamics of life; 1.6 The concept of 'functionality'; 1.7 Test tube or cell?; References; General; Advanced; Questions; For the life scientists; For the physical scientists; For those who have not made up their mind; 2 The molecules of life -- an idiot's guide; 2.1 Introduction; 2.2 The atomic components of single biological molecules.

2.3 Cell structure and sub-cellular architecture2.4 Amino acids, peptides and proteins; 2.5 Sugars; 2.6 Nucleic acids; 2.7 Lipids; 2.8 Miscellaneous 'small' molecules; 2.9 The 'central dogma' of molecular biology; 2.10 Molecular simulations; 2.11 Importance of non-covalent forces; References; General; Advanced; Questions; For the life scientists; For the physical scientists; For those who have not made up their mind; 3 Making the invisible visible: part 1 -- methods that use visible light; 3.1 Introduction; 3.2 Magnifying images.

3.3 Generating optical contrast using scattered light or fluorescence3.4 Organic dyes, FlAsH/ReAsH, fluorescent amino acids and quantum dots; 3.5 Fluorescent proteins, SNAP/CLIP-Tags and HaloTags; 3.6 Illuminating and detecting fluorescent tags; 3.6.1 Widefield modes of epifluorescence and oblique epifluorescence; 3.6.2 Slimfield and narrow-field epifluorescence; 3.6.3 Confocal microscopy; 3.6.4 Multi-photon excitation; 3.6.5 Optical lock-in detection; 3.6.6 Light sheet microscopy -- SPIM; 3.6.7 Adaptive optics; 3.7 Fluorescence correlation spectrosopy (FCS).

3.8 Fluorescence lifetime imaging (FLIM)3.9 'Super-resolution' techniques; 3.9.1 Iterative fitting (FIONA-type) approaches; 3.9.2 Sub-stoichiometric labelling; 3.9.3 Total internal reflection fluorescence (TIRF); 3.9.4 Stochastic activation, switching and blinking of fluorescent dyes; 3.9.5 Shrinking the point spread function; 3.9.6 Near-field approaches; 3.9.7 Structured illumination; 3.9.8 Förster resonance energy transfer; 3.10 'Multi-dimensional' imaging; References; General; Advanced; Questions; For the life scientists; For the physical scientists.

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