TY - BOOK AU - Dandekar,Thomas AU - Naseem,Muhammad TI - Auxins and cytokinins in plant biology: methods and protocols T2 - Methods in molecular biology SN - 1493968297 AV - QK898.A8 U1 - 572.8/2 PY - 2017///] CY - New York PB - Humana Press KW - Auxin KW - Cytokinins KW - Indoleacetic Acids KW - Plant Growth Regulators N1 - Includes bibliographical references and index; Methodological advances in auxin and cytokinin biology / Andrej Hurný and Eva Benková -- Analytical determination of auxins and cytokinins / Petre I. Dobrev, Klára Hoyerová, and Jan Petrášek -- Manipulation of auxin and cytokinin balance during the Plasmodiophora brassicae-Arabidopsis thaliana interaction / Jutta Ludwig-Müller, Susann Auer, Sabine Jülke, and Sabine Marschollek -- Microbial manipulation of auxins and cytokinins in plants / Anwar Hussain, Ihsan Ullah, and Shahida Hasnain -- Standardized method to assess infection rates of root-knot and cyst nematodes in Arabidopsis thaliana mutants with alterations in root development related to auxin and cytokinin signaling / Rocío Olmo, Ana Cláudia Silva, Fernando E. Díaz-Manzano, Javier Cabrera, Carmen Fenoll, and Carolina Escobar Reconstruction of an immune dynamic model to simulate the contrasting role of auxin and cytokinin in plant immunity -- Interplay between auxin and cytokinin and its impact on mitogen activated protein kinase (MAPK) / Pallavi Singh and Alok Krishna Sinha -- Quantification of cytokinin levels and responses in abiotic stresses / Alfonso Albacete -- Assessment of cytokinin-induced immunity through quantification of Hyaloperonospora arabidopsidis infection in Arabidopsis thaliana / Ruth A. Watson and Cristiana T. Argueso -- Real-time genetic manipulations of the cytokinin pathway : a tool for laboratory and field studies / Martin Schäfer and Stefan Meldau -- Modulating the levels of plant hormone cytokinins at the host-pathogen interface / Muhammad Naseem, Shabana Shams, and Thomas Roitsch -- Analyzing cytokinin responses during plant-nematode interactions / Florian M.W. Grundler and Shahid Siddique -- Examining H2O2 Production in Arabidopsis leaves upon challenge by cytokinin / Shahjahan Shabbir Ahmed, Ihsan Ullah, Shazia Irfan, and Nazeer Ahmed -- Systems biology methodology combining transcriptome and interactome datasets to assess the implications of cytokinin signaling for plant immune networks / Meik Kunz, Thomas Dandekar, and Muhammad Naseem -- Monitoring of crosstalk between jasmonate and auxin in the framework of plant stress responses of roots / Víctor Carrasco Loba, Marta-Marina Pérez Alonso, and Stephan Pollmann -- High-throughput protoplast trans-activation (PTA) screening to define transcription factors in auxin-mediated gene regulation / Nora Wehner, Jörn Herfert, Wolfgang Dröge-Laser, and Christoph Weiste N2 - This volume focuses and describes tools, assays, and techniques used to enhance the understanding of the role of auxins and cytokinins. The chapters in this book cover topics such as: microbial manipulation of auxin and cytokinins in plants; interplay between auxin and cytokinin and its impact on MAPK; H2O2 production in Arabidopsis leaves; crosstalk between jasmonate and auxin in plant stress responses of roots; and high-throughput protoplast trans-activation (PTA) screening. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Auxins and Cytokinins in Plant Biology: Methods and Protocols is a valuable resource to plant scientists, graduate and under graduate students in addressing their biological questions relevant to the functional implications of auxin and cytokinins UR - http://deposit.d-nb.de/cgi-bin/dokserv?id=870b45fdab3f46ad8d2742795897cc3f&prov=M&dok_var=1&dok_ext=htm UR - http://www.springer.com/us/book/9781493968299 ER -