TY - BOOK AU - Adley,Catherine TI - Food-borne pathogens: methods and protocols T2 - Methods in biotechnology SN - 158829465X AV - QR201.F62 F65 2006 U1 - 616.9 22 PY - 2006/// CY - Totowa, N.J. PB - Humana Press KW - Foodborne diseases KW - Molecular diagnosis KW - Food KW - Microbiology KW - Molecular microbiology KW - Food Microbiology KW - Food Analysis KW - methods KW - Food Contamination KW - prevention & control KW - Foodborne Diseases KW - Maladies d'origine alimentaire KW - Diagnostic moléculaire KW - Microbiologie moléculaire KW - Aliments KW - Microbiologie KW - MEDICAL KW - Infectious Diseases KW - bisacsh KW - HEALTH & FITNESS KW - Diseases KW - Contagious KW - fast KW - Laboratory Manual KW - Laboratory manuals KW - lcgft KW - Manuels de laboratoire KW - rvmgf N1 - Includes bibliographical references and index; Detection of hemolysins in Aeromonas spp. isolates from food sources / Rosabel Falcón [and others] -- Detection and purification of Bacillus cereus enterotoxins / Toril Lindbäck and Per Einar Granum -- Campylobacter / Rachel Gorman and Catherine C. Adley -- Detection of Clostridium botulinum by multiplex PCR in foods and feces / Miia Lindström, Mari Nevas, and Hannu Korkeala -- Multiplex PCR for the specific identification of enterohemorrhagic Escherichia coli strains in the O157:H7 complex / Peter C.H. Feng and Steven R. Monday -- Pulsenet's step-by-step laboratory protocol for molecular subtyping of Listeria monocytogenes by macrorestriction and pulsed-field gel electrophoresis / Lewis M. Graves and Balasubramanian Swaminathan; Plesiomonas shigelloides / Ivan Ciznar [and others] -- Pulsed-field gel electrophoresis as a molecular technique in Salmonella epidemiological studies / Rachel Gorman and Catherine C. Adley -- Kits for the detection of food poisoning toxins produced by Bacillus cereus and Staphylococcus aureus / Moira M. Brett -- Microbiological and molecular methods to identify and characterize toxigenic Vibrio cholerae from food samples / Keya De, Ranjan K. Nandy, and G. Balakrish Nair -- HPLC measurement of aflatoxin B1 and metabolites in isolated rat hepatocytes / Jennifer Colford -- Detection of noroviruses of genogroups I and II in drinking water by real-time one-step rRT-PCR / Christian M. Beuret -- Detection of enteroviruses / Miguel-Angel Jiménez-Clavero [and others]; Detection of hepatitis A virus and rotavirus using nucleic acid sequence-based amplification / Julie Jean and Ismaïl Fliss -- Isolation and characterization of cathepsin-L1 protease from Fasciola hepatica excretory/secretory products for the serodiagnosis of human fasciolosis / Sandra O'Neill, Grace Mulcahy, and John Dalton -- Molecular biology methods for detection and identification of Cryptososporidium species in feces, water and shellfish / C.J.P. Lowery [and others] -- Molecular identification of nematode worms from seafood (Anisakis spp. and Pseudoterranova spp.) and meat (Trichinella spp.) / Giuseppe La Rosa, Stefano D'Amelio, and Eduardo Pozio -- Approaches to developing quantitative risk assessment models / Enda J. Cummins -- A review of surveillance networks of food-borne diseases / Camelia Molnar, Rita Wels, and Catherine C. Adley N2 - The large-scale production and distribution of prepared foods have made rapid and accurate biological testing a daily necessity for the food industry. In Food-Borne Pathogens: Methods and Protocols, expert laboratory scientists describe in a readily reproducible format the classic and emerging molecular methods for the laboratory isolation and identification of the pathogens, viruses, and parasites that cause food-borne disease. Among the pathogens covered are specific bacteria, including Salmonella spp., Campylobacter spp., Listeria spp., and Bacillus spp.; viruses, including noroviruses and enteroviruses, and parasites, including Cryptosporidium and seafood nematode worms. The authors also review toxin detection kits, the use of high performance liquid chromatography, the proper control of bacterial storage conditions, and the importance of surveillance systems and risk assessment. The protocols follow the successful Methods in Biotechnology series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and highly practical, Food-Borne Pathogens: Methods and Protocols offers food technologists and research scientists alike an accessible source of molecular techniques currently being used and new methodologies for testing organisms that might not have been attempted previously UR - https://link-springer-com.libraryproxy.ist.ac.at/10.1385/1592599907 ER -