TY  - BOOK
AU  - Scott,M.Paul
TI  - Transgenic maize: methods and protocols 
T2  - Methods in molecular biology,
SN  - 9781934115497
AV  - SB191.M2 T725 2009
U1  - 633.15233 22
PY  - 2009///
CY  - New York, NY
PB  - Humana
KW  - Corn
KW  - Genetic engineering
KW  - Transgenic plants
KW  - Zea mays
KW  - genetics
KW  - Plants, Genetically Modified
KW  - Plantes transgéniques
KW  - cct
KW  - fast
KW  - Laboratory Manual
KW  - Laboratory manuals
KW  - lcgft
KW  - Manuels de laboratoire
KW  - rvmgf
N1  - Includes bibliographical references and index; Design of gene constructs for transgenic maize / D. Liu -- Transient expression of GFP in immature seed tissues / C.T. Shepherd -- Biolistic gun-mediated maize genetic transformation / K. Wang and B. Frame -- Agrobacterium-mediated maize transformation: Immature embryos versus callus / V. Sidorov and D. Duncan -- Whiskers-mediated maize transformation / J.F. Petolino and N.L. Arnold -- Methods for generation and analysis of fluorescent protein-tagged maize lines / A. Mohanty [and others] -- Use of transgene-induced RNAi to regulate endogenous gene expression / K. McGinnis -- Plasmid rescue: Recovery of flanking genomic sequences from transgenic transposon insertion sites / G.-L. Nan and V. Walbot -- Nonradioactive genomic DNA blots for detection of low abundant sequences in transgenic maize / G.-L. Nan and V. Walbot -- Tissue-print immunodetection of transgene products in endosperm for high-throughput screening of seeds / M.P. Scott -- Determination of transgene copy number by real-time quantitative PCR / C.T. Shepherd, A.N. Moran Lauter and M.P. Scott -- Herbicide resistance screening assay / J.M. Peterson -- Characterizing transgene inheritance / E.H. Bicar -- Backcross breeding / K.E. Vogel
N2  - As increasing global population and continuing economic development ensure the need for further production and cultivation of maize, the necessity of the application of transgenic technology to this model species and crop plant grows steadily. In Transgenic Maize: Methods and Protocols, experts in the various disciplines of the field contribute readily reproducible protocols covering such topics as transformation methods, transgenic maize in research, the analysis of transgenic plants, and breeding with transgenes. Written in the highly successful Methods in Molecular Biology series format, the chapters present brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Cutting-edge and easy to use, Transgenic Maize: Methods and Protocols will greatly aid researchers wishing to use these procedures in their own laboratories, and will provide readers with a better understanding of the experiments being performed by cooperators or fee-for-service labs
UR  - https://link-springer-com.libraryproxy.ist.ac.at/10.1007/978-1-59745-494-0
ER  -