TY  - BOOK
AU  - Hirschey,Matthew D.
TI  - Sirtuins: methods and protocols 
T2  - Methods in Molecular Biology, Methods and Protocols,
SN  - 9781627036375
AV  - QP551 
U1  - 572/.6 23
PY  - 2013///
CY  - New York
PB  - Humana Press
KW  - Sirtuins
KW  - Laboratory manuals
KW  - NAD-ADP-ribosyltransferase
KW  - Histone deacetylase
KW  - Proteins
KW  - Glycosyltransferases
KW  - Transferases
KW  - Hydrolases
KW  - Enzymes
KW  - Group III Histone Deacetylases
KW  - Intracellular Signaling Peptides and Proteins
KW  - ADP Ribose Transferases
KW  - Histone Deacetylases
KW  - Pentosyltransferases
KW  - Amino Acids, Peptides, and Proteins
KW  - Amidohydrolases
KW  - Chemicals and Drugs
KW  - Enzymes and Coenzymes
KW  - Poly (ADP-ribose) polymérase
KW  - Histone désacétylase
KW  - Protéines
KW  - Pentosyltransférases
KW  - Glycosyltransférases
KW  - Transférases
KW  - protein
KW  - aat
KW  - enzyme
KW  - fast
KW  - genen
KW  - genes
KW  - genomica
KW  - genomics
KW  - diergenetica
KW  - animal genetics
KW  - eiwitexpressieanalyse
KW  - proteomics
KW  - Molecular Biology (General)
KW  - Moleculaire biologie (algemeen)
KW  - Laboratory Manual
KW  - lcgft
KW  - Manuels de laboratoire
KW  - rvmgf
N1  - Includes index; Includes bibliographical references and index; Introduction : sirtuins in aging and diseases; Leonard Guarente --; Sirtuins in yeast : phenotypes and tools; Scott Tsuchiyama [and others] --; C. elegans sirtuins; Mohan Viswanathan and Heidi A. Tissenbaum --; Genetic and biochemical tools for investigating sirtuin function in Drosophila melanogaster; Jason G. Wood, Rachel Whitaker, and Stephen L. Helfand --; Generating mammalian sirtuin tools for protein- interaction analysis; Kathleen A. Hershberger [and others] --; Mass spectrometry-based detection of protein acetylation; Yu Li [and others] --; SILAC-based quantification of Sirt1-responsive lysine acetylome; Yue Chen, Gozde Colak, and Yingming Zhao --; Targeted quantitation of acetylated lysine peptides by selected reaction monitoring mass spectrometry; Matthew J. Rardin, Jason M. Held, and Bradford W. Gibson --; Identification of deacetylase substrates with the biotin switch approach; J. Will Thompson, Alex Robeson, and Joshua L. Andersen --; Assaying chromatin sirtuins; Lei Zhong [and others] --; Measurement of sirtuin enzyme activity using a substrate-agnostic fluorometric nicotinamide assay; Basil P. Hubbard and David A. Sinclair --; Detecting sirtuin-catalyzed deacylation reactions using 32P-labeled NAD and thin-layer chromatography; Anita Zhu, Xiaoyang Su, and Hening Lin --; Chemical acetylation and deacetylation; Kristofer S. Fritz --; Accurate measurement of nicotinamide adenine dinucleotide (NAD+) with high-performance liquid chromatography; Jun Yoshino and Shin-ichiro Imai --; In vivo measurement of the acetylation state of sirtuin substrates as a proxy for sirtuin activity; John Dominy, Pere Puigserver, and Carles Cantó --; Oxygen flux analysis to understand the biological function of sirtuins; Dongning Wang [and others] --; Emerging links between sirtuins and autophagy; In Hye Lee, Jeanho Yun, and Toren Finkel --; Methods to study the role of sirtuins in genome stability; Paloma Martínez-Redondo and Alejandro Vaquero --; Circadian measurements of sirtuin biology; Kathryn Moynihan Ramsey [and others] --; Utilizing calorie restriction to evaluate the role of sirtuins in healthspan and lifespan of mice; Jessica Curtis and Rafael de Cabo
N2  - Featuring a diverse array of model organisms and scientific techniques, Sirtuins: Methods and Protocols collects detailed contributions from experts in the field addressing this vital family of genes. Opening with methods to generate sirtuin biology tools, the book continues by covering methods to identify sirtuin substrates, to measure sirtuin activity, and to study sirtuin biology. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and easy to use, Sirtuins: Methods and Protocols presents detailed protocols for sirtuin research that can be followed directly or modified to investigate new areas of sirtuin biology
UR  - https://link-springer-com.libraryproxy.ist.ac.at/10.1007/978-1-62703-637-5
ER  -