TY  - BOOK
AU  - Lu,Long Jason
TI  - Gene essentiality: methods and protocols 
T2  - Methods in Molecular Biology,
SN  - 9781493923984
AV  - QH434 
U1  - 579.5 23
PY  - 2015///]
CY  - New York, NY
PB  - Humana Press
KW  - Microbial genomics
KW  - Genomics
KW  - Genes
KW  - Genomes
KW  - Genetics
KW  - Technique
KW  - Investigative Techniques
KW  - Publication Formats
KW  - Publication Characteristics
KW  - Genome Components
KW  - Analytical, Diagnostic and Therapeutic Techniques and Equipment
KW  - Genome
KW  - Genetic Structures
KW  - Genetic Phenomena
KW  - Phenomena and Processes
KW  - Laboratory Manuals
KW  - Genes, Essential
KW  - Genetic Techniques
KW  - Génomique microbienne
KW  - Génomique
KW  - Gènes
KW  - Génomes
KW  - Génétique
KW  - Microbiology (non-medical)
KW  - bicssc
KW  - Medical genetics
KW  - Science
KW  - Life Sciences
KW  - Biology
KW  - Microbiology
KW  - bisacsh
KW  - Medical
KW  - fast
KW  - biomedische wetenschappen
KW  - biomedicine
KW  - microorganisms
KW  - genomica
KW  - genomics
KW  - humane genetica
KW  - human genetics
KW  - Medicine (General)
KW  - Geneeskunde (algemeen)
KW  - Laboratory Manual
KW  - Internet Resources
KW  - Laboratory manuals
KW  - lcgft
KW  - Manuels de laboratoire
KW  - rvmgf
N1  - Includes bibliographical references and index; Microarray Transposon Tracking for the Mapping of Conditionally Essential Genes in Campylobacter jejuni --; Identifying Essential Streptococcus sanguinis Genes Using Genome-Wide Deletion Mutation --; Defining Essential Genes and Identifying Virulence Factors of Porphyromonas gingivalis by Massively-Parallel Sequencing of Transposon Libraries (Tn-seq) --; Identification of Essential Genes and Synthetic Lethal Gene Combinations in Escherichia coli K-12 --; Identification of Genes Essential for Leptospirosis --; Identifying Essential Genes in Mycobacterium tuberculosis by Global Phenotypic Profiling --; Essential Genes in the Infection Model of Pseudomonas aeruginosa-PCR-Based Signature-Tagged Mutagenesis --; Genome-Wide Synthetic Genetic Screening by Transposon Mutagenesis in Candida albicans --; An Integrated Machine-Learning Model to Predict Prokaryotic Essential Genes --; A Statistical Framework for Improving Genomic Annotations of Transposon Mutagenesis (TM) Assigned Essential Genes --; A Proposed Essential Gene Discovery Pipeline: A Campylobacter jejuni Case Study --; Computational Prediction of Essential Metabolic Genes Using Constraint-Based Approaches --; Three Computational Tools for Predicting Bacterial Essential Genes --; Gene Essentiality Analysis Based on DEG 10, an Updated Database of Essential Genes --; A Novel Essential Domain Perspective for Exploring Gene Essentiality
N2  - This volume opens by covering two main types of approaches widely used to determine essential genes: single-gene knockouts and transposon mutagenesis, in both prokaryotes and "Candida albicans." Given the significant advancement in the computational predictions of microbial essential genes, the second half of the book examines four main types of approaches: comparative genomics, supervised machine learning, constraint-based methods, and corrections of transposon mutagenesis data, as well as databases and servers that are often used in studying gene essentiality. Written in the highly successful "Methods in Molecular Biology" series format, chapters include an introduction to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, "Gene Essentiality: Methods and Protocols" will aid researchers who wish to further our knowledge in this vital field of study
UR  - https://link-springer-com.libraryproxy.ist.ac.at/10.1007/978-1-4939-2398-4
ER  -