TY - BOOK AU - McGenity,Terry J. AU - Timmis,K.N. AU - Nogales,Balbina TI - Hydrocarbon and lipid microbiology protocols: biochemical methods T2 - Springer protocols handbooks, SN - 9783662491379 AV - QP751 U1 - 572/.57 23 PY - 2016/// CY - Berlin PB - Springer KW - Lipids KW - Laboratory manuals KW - Hydrocarbons KW - Analysis KW - Microbiology KW - Synthetic biology KW - Systems biology KW - Synthetic Biology KW - Systems Biology KW - Lipides KW - Manuels de laboratoire KW - Hydrocarbures KW - Analyse KW - Microbiologie KW - Biologie synthétique KW - Biologie systémique KW - lipid KW - aat KW - microbiology KW - fast KW - Laboratory Manual KW - lcgft KW - rvmgf N1 - Includes bibliographical references; Introduction -- Protocols for the isolation and analysis of lipopeptides and bioemulsifiers -- Protocols for the detection and chemical characterisation of microbial glycolipids -- Isolation of glycoprotein bioemulsifiers produced by marine bacteria -- Protocols for lipid extraction from wet algal biomass -- Isolation and characterization of lipid droplets from yeast -- Protocols for Isolation and Analysis of Polyhydroxyalkanoates -- Purification of lipid rafts from bacterial membranes -- Isolation and analysis of membrane phospholipids as biomarkers -- Protocols for purifying and characterizing integral membrane AlkB enzymes -- Protocols for structural and functional analysis of particulate methane monooxygenase from Methylocystis species strain Rockwell (ATCC 49242) -- Phenotype-based Identification of key enzymes for polycyclic aromatic hydrocarbon (PAH) metabolism from Mycobacteria using transposon mutagenesis and a PAH spray plate -- Microcalorimetry as a general technique to characterize ligand binding N2 - This volume provides protocols for the biochemical analysis of hydrocarbon - and lipid - relevant products, cell components and activities of microbes that interact with hydrophobic compounds. It includes methods for the extraction, purification and characterisation of surface tension-reducing bioemulsifiers and biosurfactants that increase the surface area and hence bioavailability of hydrophobic substrates UR - https://link-springer-com.libraryproxy.ist.ac.at/10.1007/978-3-662-49137-9 ER -