TY - BOOK AU - Hehl,Reinhard TI - Plant synthetic promoters: methods and protocols T2 - Methods in molecular biology, SN - 9781493963966 AV - QK981 U1 - 572.8/652 23 PY - 2016/// CY - New York, NY PB - Humana Press KW - Plant gene expression KW - Plant genetic transformation KW - Promoters (Genetics) KW - Genetics KW - Technique KW - Gene Expression Regulation, Plant KW - Promoter Regions, Genetic KW - Plants KW - genetics KW - Regulatory Elements, Transcriptional KW - Genetic Techniques KW - Plantes KW - Expression génique KW - Transformation génétique végétale KW - Promoteurs (Génétique) KW - Génétique KW - Botany & plant sciences KW - bicssc KW - Science KW - Life Sciences KW - Botany KW - bisacsh KW - fast KW - Laboratory Manual KW - Laboratory manuals KW - lcgft KW - Manuels de laboratoire KW - rvmgf N1 - Includes index; Includes bibliographical references and index; What Have We Learned About Synthetic Promoter Construction? -- Quantitative Analysis of Cis-Regulatory Element Activity Using Synthetic Promoters in Transgenic Plants -- The Identification of Cis-Regulatory Sequence Motifs in Gene Promoters Based on SNP Information -- Quantitative Analysis of Protein-DNA Interaction by qDPI-ELISA -- Analyzing Synthetic Promoters Using Arabidopsis Protoplasts -- Selecting Hypomethylated Genomic Regions Using MRE-Seq -- Spatio-Temporal Imaging of Promoter Activity in Intact Plant Tissues -- Novel Synthetic Promoters from the Cestrum Yellow Leaf-Curling Virus -- Fast and Efficient Cloning of Cis-Regulatory Sequences for High Throughput Yeast One-Hybrid Analyses of Transcription Factors -- The Physcomitrella patens System for Transient Gene Expression Assays -- Analysis of Microbe-Associated Molecular Pattern-Responsive Synthetic Promoters with the Parsley Protoplast System -- A Framework for Discovering, Designing, and Testing MicroProteins to Regulate Synthetic Transcriptional Modules -- Simultaneous Analysis of Multiple Promoters: An Application of the PC-GW Binary Vector Series -- GenoCAD Plant Grammar to Design Plant Expression Vectors for Promoter Analysis -- Bioinformatic Identification of Conserved Cis-Sequences in Coregulated Genes -- In Silico Expression Analysis -- FootprintDB: Analysis of Plant Cis-Regulatory Elements, Transcription Factors, and Binding Interfaces -- RSAT::Plants: Motif Discovery Within Clusters of Upstream Sequences in Plant Genomes -- RSAT::Plants: Motif Discovery in ChIP-Seq Peaks of Plant Genomes N2 - This text assembles experimental and bioinformatics protocols for the design and experimental testing of synthetic promoters. The identification of cis -regulatory elements potentially achieving the desired expression of a gene is at the core of synthetic promoter design. For this, several bioinformatics chapters are presented UR - https://link-springer-com.libraryproxy.ist.ac.at/10.1007/978-1-4939-6396-6 ER -