TY  - BOOK
AU  - Walter,Harry
AU  - Johansson,Göte
TI  - Aqueous two-phase systems
T2  - Methods in enzymology,
SN  - 0121821293
AV  - QP601 .M49 vol. 228
U1  - 660.6 20
PY  - 1994///
CY  - San Diego
PB  - Academic Press
KW  - Separation (Technology)
KW  - Phase partition
KW  - Biochemistry
KW  - Technique
KW  - Biotechnology
KW  - Cell Fractionation
KW  - methods
KW  - Cell Extracts
KW  - isolation & purification
KW  - Séparation (Technologie)
KW  - Partage des phases
KW  - Biochimie
KW  - Biotechnologie
KW  - fast
KW  - Polymers
KW  - Electronic books
N1  - Includes bibliographical references and indexes; Section I. General methodology and apparatus --; Section II. Partitioning and affinity partitioning of macromolecules --; A. Proteins --; B. Nucleic acids --; Section III. Studies on protein interactions, molecular structure, charge, hydrophobicity, and conformational changes --; Section IV. Partitioning and affinity partitioning of particulates --; A. Cells: Separation and fractionation of cell populations and obtaining information on their surface properties -- Affinity partitioning --; B. Organelles: Separation and subfractionation --; C. Membranes: Separation and subfractionation -- Affinity partitioning -- Membrane domain analysis --; Section V. Aqueous phase separations in biological systems --; Section VI. Aqueous two-phase systems in large-scale processes: Biotechnology --; A. Extractive bioconversions --; B. Proteins: Downstream processing --; C. Design of proteins for enhanced extraction --; D. Other applications of aqueous phases in biotechnology
N2  - This volume of Methods in Enzymology represents the sole collection of separation methods based on aqueous two-phase systems. It includes procedures for the isolation of proteins, especially enzymes, nucleic acids, cell membranes, and organelles, as well as for the separation and study of cells. Key Features * Use of affinity partitioning for enzyme purification * Separation and study of cells * Isolation of plasma membranes * Large scale biotechnical use
UR  - https://www-sciencedirect-com.libraryproxy.ist.ac.at/science/bookseries/00766879/228
ER  -