TY  - BOOK
AU  - Shukla,Arun Kumar
TI  - Membrane proteins: Production and functional characterization 
T2  - Methods in enzymology
SN  - 0128016256
AV  - QP552.M44 M45 2015
U1  - 572/.696 23
PY  - 2015///
CY  - Amsterdam, Netherlands, Boston, Mass.
PB  - Elsevier/ Academic Press
KW  - Membrane proteins
KW  - Recombinant proteins
KW  - Membrane Proteins
KW  - Recombinant Proteins
KW  - Protéines membranaires
KW  - Protéines recombinantes
KW  - SCIENCE
KW  - Life Sciences
KW  - Biochemistry
KW  - bisacsh
KW  - fast
KW  - Internet Resources
KW  - Plates
N1  - Includes bibliographical references and indexes; Engineering Escherichia coli for functional expression of membrane proteins -- ACEMBLing a multiprotein transmembrane complex: the functional SecYEG-SecDF-YajC-YidC holotranslocon protein secretase/insertase -- Expression of purification of OsVDAC4 -- Membrane protein expression in Lactococcus lactis -- An unconventional anaerobic membrane protein production system based on Wolinella succinogenes -- Membrane protein expression and analysis in yeast -- Heterologous expression of G-protein-coupled receptors in yeast -- Recombinant G protein-coupled receptor expression in Saccharomyces cerevisiae for protein characterization -- Baculovirus-mediated expression of GPCRs in insect cells -- Expression of membrane proteins in the eyes of transgenic Drosophila melangaster -- Rapid method to express and purify human membrane protein using the Xenopus Oocyte system for functional and low-resolution structural anlaysis -- Expression of G protein-coupled receptors in mammalian cells -- Construction of stable mammalian cell lines for inducible expression of G protein-coupled receptors -- Rapid and facile recombinant expression of bovine rhodopsin in HEK293S GnTI- cells using a PiggyBac inducible system -- Semliki forest virus-based expression of recombinant GPCRs; Screening for lipid requirements of membrane proteins by combining cell-free expression with nanodiscs -- Liposome reconstitution and transport assay for recombinant transporters -- Lipid reconstitution and recording of recombinant ion channels -- Reconstitution of membrane proteins: a GPCR as an example -- Ion channel reporter for monitoring the activity of engineered GPCRs -- Methods for labeling skeletal muscle ion channels site-specifically with fluorophores suitable for FRET-based structural analysis -- Structural and fucntional studies of NirC from Salmonella typhimurium -- Surface plasmon resonance analysis of seven-transmembrane receptors -- Cross-linking strategies to study peptide ligand-receptor ineractions -- From recombinant expression to crystals: a step-by-step guide to GPCR crystallography -- Structure-based biophysical analysis of the interaction of rhodopsin with G protein and arrestin
N2  - Membrane Proteins - Production and Function Characterization a volume of Methods in Enzymology, encompasses chapters from the leading experts in the area of membrane protein biology. The chapters provide a brief overview of the topics covered and also outline step-by-step protocol. Illustrations and case example images are included wherever appropriate to help the readers understand the schematics and general experimental outlines
UR  - https://www-sciencedirect-com.libraryproxy.ist.ac.at/science/bookseries/00766879/556
ER  -