Molecular diagnosis of cancer : methods and protocols / edited by Joseph E. Roulston and John M.S. Bartlett.

Prognostic and predictive factors / M. Scott and P.A. Hall -- Assessment of predictive values of tumor markers / J.E. Roulston -- Quality assurance of predictive markers in breast cancer / A. Rhodes and D.M. Barnes -- Extraction of nucleic acid templates / J.M. Bartlett and H. Speirs -- Microdissection and extraction of DNA from archival tissue / J. Edwards, J.J. Going and J.M. Bartlett -- Fluorescence in situ hybridization: technical overview / J.M. Bartlett -- HER2 FISH in breast cancer / J.M. Bartlett and A. Forsyth -- Fluorescence in situ hybridization for BCR-ABL / M.W. Drummond [and others] -- UroVysion multiprobe FISH in urinary cytology / L. Bubendorf and B. Grilli -- Chromogenic in situ hybridization in tumor pathology / J. Isola and M. Tanner -- Comparative genomic hybridization and fluorescence in situ hybridization in chronic lymphocytic leukemia / M. Jarosova -- Molecular characterization of human papillomaviruses by PCR and in situ hybridization / S.D. Vernon and E.R. Unger -- A nested RT-PCR assay to detect BCR/abl / L.M. Wasserman -- TP53 mutation detection by SSCP and sequencing / J. Hakkarainen, J.A. Welsh and K.H. Vahakangas -- PCR diagnosis of T-cell lymphoma in paraffin-embedded bone marrow biopsies / J. Benhattar and S. Gebhard -- Circulating DNA analysis: protocols and clinical applications using TaqMan assays / K.C. Chan and Y.M. Lo -- Microsatellite instability: theory and methods / G. Gifford and R. Brown -- Diagnostic and prognostic significance of the methylation status of Myf-3 in lymphoproliferative disorders / J.M. Taylor, P.H. Kay and D.V. Spagnolo -- Quantitative analysis of PRAME for detection of minimal residual disease in leukemia / M. Matsushita, R. Yamazaki and Y. Kawakami -- Determination of cyclin d1 expression by quantitative real-time, reverse-transcriptase polymerase chain reaction / K.E. Bijwaard and J.H. Lichy -- Detection of telomerase hTERT gene expression and its splice variants by RT-PCR / W.N. Keith and S.F. Hoare -- Detection of telomerase enzyme activity by TRAP assay / W.N. Keith and A.J. Monaghan -- Identification of TP53 mutations in human cancers using oligonucleotide microarrays / W.H. Wen and M.F. Press -- Detection of K-ras mutations by a microelectronic DNA chip / E. Lopez-Crapez [and others] -- Microarray-based CGH in cancer / E. Pestova, K. Wilber and W. King -- Tissue microarrays / R. Simon, M. Mirlacher and G. Sauter.

Includes bibliographical references and index.

Print version record.

The growing tide of novel therapeutics that target molecules within a specific individual's cancer cells-over 300 such agents are currently in phase I or II clinical trials-makes it essential to employ appropriate diagnostic methods and approaches when selecting patients for treatment. In Molecular Diagnosis of Cancer: Methods and Protocols, a panel of leading international experts in molecular diagnostic medicine present a diverse collection of readily reproducible methods, derived from their own research for cancer detection. Highlights include FISH-based methodologies currently used in the diagnosis of solid tumors, the molecular diagnosis of genetic abnormalities by DNA array technologies-including sequence-specific oligonucleotide arrays and CGH arrays-and methodologies directed at the detection of epigenetic events and at quantitative gene expression. The authors apply these novel diagnostic procedures to a broad range of cases taken from cytology, solid tumor pathology, hematology, and rare cell detection, paying special attention to potential future developments and the practical problems of dealing with quality control and accuracy. The protocols presented follow the successful Methods in Molecular Medicine"!series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and highly practical, Molecular Diagnosis of Cancer: Methods and Protocols offers pathologists providing molecular diagnostic services and researchers seeking molecularly targeted therapeutic approaches a broad array of today's most powerful methods and techniques

English.