Yeast protocols : methods in cell and molecular biology / edited by Ivor H. Evans.

Includes bibliographical references and index.

Isolation and identification of yeasts from natural habitats / John F. Spencer, Dorothy M. Spencer -- Maintenance and culture of yeasts / John F. Spencer, Dorothy M. Spencer -- Mutagenesis in yeast / John F. Spencer, Dorothy M. Spencer -- Rare-mating and cytoduction in Saccharomyces cerevisiae / John F. Spencer, Dorothy M. Spencer -- Protoplast fusion in Saccharomyces cerevisiae / Brendan P. Curran, Virginia C. Bugeja -- Meiotic analysis / John F. Spencer, Dorothy M. Spencer -- Ascus dissection / Carl Saunders-Singer -- Karyotyping of yeast strains by pulsed-field gel electrophoresis / John R. Johnston -- Chromosomal localization of genes through pulsed-field gel electrophoresis techniques / Graham R. Bignell, Ivor H. Evans -- Isolation, purification, and analysis of nuclear DNA in yeast taxonomy / Ann Vaughan-Martini, Alessandro Martini -- Isolation of yeast DNA / Peter Piper -- Isolation of mitochondrial DNA / Graham R. Bignell, Angela R. Miller, Ivor H. Evans -- Isolation of yeast plasma membranes / Barry Panaretou, Peter Piper -- The extraction and analysis of sterols from yeast / Michael A. Quail, Steven L. Kelly -- Peroxisome isolation / Ben Distel, Inge Leij, Wilko Kos -- Transformation of lithium-treated yeast cells and the selection of auxotrophic and dominant markers / Robert C. Mount, Bernadette E. Jordan, Christopher Hadfield -- Biolistic transformation of yeasts / Stephen A. Johnston, Michael J. DeVit -- Genomic yeast DNA clone banks: construction and gene isolation / Graham R. Bignell, Ivor H. Evans -- Yeast colony hybridization / Matthew John Kleinman -- Determination of plasmid copy number in yeast / Bernadette E. Jordan, Robert C. Mount, Christopher Hadfield -- Determination of chromosome ploidy in yeast / Christopher Hadfield, Bernadette E. Jordan, Robert C. Mount -- Chromosome engineering in yeast with a site-specific recombination system from a heterologous yeast plasmid / Yasuji Oshima, Hiroyuki Araki, Hiroaki Matsuzaki -- Insertional mutagenesis by Ty elements in Saccharomyces cerevisiae / David J. Garfinkel -- Reporter gene systems for assaying gene expression in yeast / Robert C. Mount, Bernadette E. Jordan, Christopher Hadfield -- Preparation and use of yeast cell-free translation lysate / Alan D. Hartley, Manuel A. Santos, David R. Colthurst, Michael F. Tuite -- Electrophoretic analysis of yeast proteins / Christopher M. Grant, Ian T. Fitch, Michael F. Tuite -- Preparation of total RNA / Alistair J. Brown -- mRNA abundance and half-life measurements / Alistair J. Brown, Francis A. Sagliocco -- Polysome analysis / Francis A. Sagliocco, Paul A. Moore, Alistair J. Brown -- Induction of heat shock proteins and thermotolerance / Peter Piper -- Rhodamine B assay for estimating activity of killer toxins permeabilizing cytoplasmic membranes / Vladimír Vondrejs, Zdena Palková -- Nystatin-rhodamine B assay for estimating activity of killer toxin from Kluyveromyces lactis / Zdena Palková, Vladimír Vondrejs -- Application of killer toxins in stepwise selection of hybrids and cybrids obtained by induced protoplast fusion / Vladimír Vondrejs, Zdena Palková, Zuzana Zemanová -- Killer plaque technique for selecting hybrids and cybrids obtained by induced protoplast fusion / Zdena Palková, Vladimír Vondrejs -- Genotoxicity testing in Schizosaccharomyces pombe / Pamela McAthey -- Purification and quantification of Saccharomyces cerevisiae cytochrome P450 / Ian Stansfield, Steven L. Kelly -- Calorimetry of whole yeast cells / Linda J. Ashby, Anthony E. Beezer -- Light microscopy methods / Eva Streiblová, Jiří Hašek -- Fluorescence microscopy methods / Jiří Hašek, Eva Streiblová -- Immunoelectron microscopy / Ever-Jan Tuinen.

Print version record.

Yeast Protocols intends to offer a selection of well-proven proto­ cols in cell and molecular biology, applicable to yeasts including, but certainly not exclusively aimed at, Saccharomyces cerevisiae. Saccharomyces cerevisiae and its very distant cousin, Schizosacc- romyces pombe, are of course now foremost model eukaryotic organisms, and the focus of wide-ranging experimental studies, especially those using molecular genetic techniques. Many of the lat­ ter, such as DNA sequencing and in vitro mutagenesis, are general DNA techniques, well covered in other volumes of Humana's Meth­ ods in Molecular Biology series, and elsewhere. The inclusion of a number of non-DNA techniques in this book is meant to reflect the resurgent interest in yeast cell biology sparked by the development of gene manipulation methods-for example, cellular localization of cloned gene products using microscopical techniques. The presentation of protocols follows the successful Methods in Molecular Biology series format, with a clear sequence of steps and extensive troubleshooting notes. It is our hope that these protocols will be useful not only to established members of the full-time research community, but also to the less experienced-first degree level and masters students undertaking project work, as well as PhD students starting their experimental programs; I am well aware that these young apprentice scientists are not always able to receive the supervision time that they, and indeed their supervisors, would like.

English.