Developmental hematopoiesis : methods and protocols / edited by Margaret H. Baron.

Includes bibliographical references and index.

Genetic approaches -- Analysis of cell fate outcomes in transplantation models -- Animal models -- Bioinformatics and functional genomics.

Gene-targeting and transgenic strategies for the analysis of hematopoietic development in the mouse / Hanna K.A. Mikkola and Stuart H. Orkin -- Inducible transgene expression in mouse ES cells / David T. Ting, Michael Kyba, and George Q. Daley -- Quantitative trait analysis in the investigation of function and aging in hematopoietic stem cells / Hans-Willem Snoeck -- Flow cytometric analysis of hematopoietic development / Edward F. Srour and Mervin C. Yoder -- Reconstitution of hematopoiesis following intrauterine transplantation of stem cells / Elizsabeth H. Javazon [and others] -- Reconstitution of hematopoiesis following transplantation into neonatal mice / Scott A. Johnson and Mervin C. Yoder -- Hemocyte development during Drosophila embryogenesis / Robert A. Schulz and Nancy Fossett -- Tracking and programming early hematopoietic cells in Xenopus embryos / Maggie Walmsley, Aldo Ciau-Uitz, and Roger Patient -- Fate mapping hematopoietic lineages in the Xenopus embryo / Mary Constance Lane and Michael D. Sheets -- Analyses of immune responses to ontogeny-specific antigens using an inbred strain of Xenopus laevis (J Strain) / Yumi Izutsu and Mitsugu Mae̕no -- Use of flow cytometry and combined DNA/surface staining for analysis of hematopoietic development in the Xenopus embryo / James B. Turpen -- Analysis of hematopoietic development in the zebrafish / Noëlle N. Paffett-Lugassy and Leonard I. Zon -- Imaging early macrophage differentiation, migration, and behaviors in live zebrafish embryos / Philippe Herbomel and Jean-Pierre Levraud -- In vivo methods to analyze cell origins, migrations, homing, and interactions in the blood, vascular, and immune sytems of the avian and mammalian embryo / Franc̦oise Dieterlen-Lièvre, Sophie Creuzet, and Josselyne Salaün -- Mouse embryonic explant culture system for analysis of hematopoietic and vascular development / Margaret H. Baron and Deanna Mohn.

Hematopoietic stem cell development during mouse embryogenesis / Julien Y. Bertrand [and others] -- Analysis of hematopoietic progenitors in the mouse embryo / James Palis and Anne Koniski -- In vivo and in vitro assays of thymic organogenesis / Julie Gordon [and others] -- Retroviral transduction in fetal thymic organ culture / Bronwyn M. Owens, Robert G. Hawley, and Lisa M. Spain -- Expansion and differentiation of immature mouse and human hematopoietic progenitors / Helmut Dolznig [and others] -- A new clonal assay system for lymphoid and myeloid linages / Hiroshi Kawamoto and Yoshimoto Katsura -- Hematopoietic and endothelial development of mouse embryonic stem cells in culture / Kyunghee Choi, Yun Shin Chung, and Weh Jie Zhang -- Analysis of the vascular potential of hematopoietic stem cells / Steven M. Guthrie [and others] -- Dynamic in vivo imaging of mammalian hematovascular development using whole embryo culture / Elizabeth A.V. Jones [et al.] -- Fluorescent protein-cell labeling and its application in time-lapse analysis of hematopoietic differentiation / Matthias stadtfeld, Florencio Varas, and Thomas Graf -- Analysis of hematopoietic development during human embryonic ontogenesis / Manuela Tavian and Bruno Peault -- Hematopoietic development of human embryonic stem cells in culture / Xinghui Tian and Dan S. Kaufman -- A functional genomics approach to hematopoietic stem cell regulation / Jason A. Hackney and Kateri A. Moore.

Print version record.

Over the past decade technical and conceptual breakthroughs, cross-species comparisons, and multidisciplinary approaches have led to a virtual revolution in developmental biology. In Developmental Hematopoiesis: Methods and Protocols, a panel of expert investigators critically surveys an array of methods available to study hematopoiesis and describes in detail the powerful techniques they have established to investigate embryonic, fetal, and (to a lesser extent) postnatal blood development. The authors apply their approaches to invertebrate (fruit flies) as well as vertebrate (frog, fish, mouse, chick, and human) organisms. These state-of-the-art techniques range from the genetic, molecular, and cellular, to cell and embryo explant culture and whole animals, including in vivo imaging. Bioinformatic and functional genomic approaches for studying stem cells and their supportive stromal cells are also discussed. The protocols follow the successful Methods in Molecular Medicine series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and highly practical, Developmental Hematopoiesis: Methods and Protocols offers novice and seasoned researchers alike the critical tools needed to gain a fundamental understanding of stem cell biology-examining not only how hematopoietic stem cells form, but also how they differ in the embryo, the fetus, and the adult.

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