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Downstream processing of proteins : methods and protocols / edited by Mohamed A. Desai.

Contributor(s): Material type: TextTextSeries: Methods in biotechnology ; 9.Publication details: Totowa, N.J. : Humana Press, ©2000.Description: 1 online resource (ix, 229 pages) : illustrationsContent type:
  • text
Media type:
  • computer
Carrier type:
  • online resource
ISBN:
  • 9781592590278
  • 1592590276
  • 9780896035645
  • 0896035646
  • 1280820225
  • 9781280820229
  • 9786610820221
  • 6610820228
Subject(s): Genre/Form: Additional physical formats: Print version:: Downstream processing of proteins.DDC classification:
  • 660/.2842 22
LOC classification:
  • TP248.25.S47 D6935 2000eb
NLM classification:
  • 2002 C-471
  • QU 55
Online resources:
Contents:
Downstream Processing of Proteins -- Preface -- Contents -- Contributors -- 1 Downstream Processing in the Biotechnology Industry: An Overview -- 2 Microbial Cell Disruption by High-Pressure Homogenization -- 3 Protein Concentration and Buffer Exchange Using Ultrafiltration -- 4 High-Performance Tangential Flow Filtration for Protein Separations -- 5 Large-Scale Recovery of Protein Inclusion Bodies by Continuous Centrifugation -- 6 Application of Density Gradient Ultracentrifugation Using Zonal Rotors in the Large-Scale Purification of Biomolecules
7 Application of Chromatography in the Downstream Processing of Biomolecules8 Affinity Ligand Technology: Optimal Protein Separations for Downstream Processing -- 9 Immunoaffinity Adsorption in the Large-Scale Isolation of Biomolecules -- 10 Expanded Bed Adsorption in the Purification of Biomolecules -- 11 Application of HPLC in the Purification of Biomolecules -- 12 Countercurrent Chromatography of Proteins with Polymer Phase Systems -- 13 Practical Aspects and Applications of Radial Flow Chromatography -- 14 Sterile Filtration and Virus Filtration
15 Design and Interpretation of Viral Clearance Studies for Biopharmaceutical Products16 Validation of Sterilizing Filters in the Biotechnology Industry -- Index
In: Springer ProtocolsSummary: In Downstream Processing of Proteins: Methods and Protocols, Mohamed A. Desai and a team of experienced biotechnologists review both conventional and novel isolation techniques used in industrial applications for the downstream processing of protein molecules. These techniques include primary and secondary separations during the isolation of biomolecules, as well as unique laboratory-scale research methods with a potential for scale-up. Also treated are the various strands of the downstream biological process essential for a successful product license application, including both the validation of DSP stages, and the design and validation of viral clearance stages during the purification process. Downstream Processing of Proteins: Methods and Protocols provides scientists everywhere, but particularly in the biopharmaceutical and biotechnology industry, with a much-needed introduction to this critical technology. Every bioprocess scientist and engineer working to design and validate biological processes for novel proteins-and successfully apply for their new product licenses-will find this important book an eminently practical resource.
Holdings
Item type Current library Collection Call number Status Date due Barcode Item holds
eBook eBook e-Library eBook Protocols Available
Total holds: 0

Includes bibliographical references and index.

Print version record.

In Downstream Processing of Proteins: Methods and Protocols, Mohamed A. Desai and a team of experienced biotechnologists review both conventional and novel isolation techniques used in industrial applications for the downstream processing of protein molecules. These techniques include primary and secondary separations during the isolation of biomolecules, as well as unique laboratory-scale research methods with a potential for scale-up. Also treated are the various strands of the downstream biological process essential for a successful product license application, including both the validation of DSP stages, and the design and validation of viral clearance stages during the purification process. Downstream Processing of Proteins: Methods and Protocols provides scientists everywhere, but particularly in the biopharmaceutical and biotechnology industry, with a much-needed introduction to this critical technology. Every bioprocess scientist and engineer working to design and validate biological processes for novel proteins-and successfully apply for their new product licenses-will find this important book an eminently practical resource.

Downstream Processing of Proteins -- Preface -- Contents -- Contributors -- 1 Downstream Processing in the Biotechnology Industry: An Overview -- 2 Microbial Cell Disruption by High-Pressure Homogenization -- 3 Protein Concentration and Buffer Exchange Using Ultrafiltration -- 4 High-Performance Tangential Flow Filtration for Protein Separations -- 5 Large-Scale Recovery of Protein Inclusion Bodies by Continuous Centrifugation -- 6 Application of Density Gradient Ultracentrifugation Using Zonal Rotors in the Large-Scale Purification of Biomolecules

7 Application of Chromatography in the Downstream Processing of Biomolecules8 Affinity Ligand Technology: Optimal Protein Separations for Downstream Processing -- 9 Immunoaffinity Adsorption in the Large-Scale Isolation of Biomolecules -- 10 Expanded Bed Adsorption in the Purification of Biomolecules -- 11 Application of HPLC in the Purification of Biomolecules -- 12 Countercurrent Chromatography of Proteins with Polymer Phase Systems -- 13 Practical Aspects and Applications of Radial Flow Chromatography -- 14 Sterile Filtration and Virus Filtration

15 Design and Interpretation of Viral Clearance Studies for Biopharmaceutical Products16 Validation of Sterilizing Filters in the Biotechnology Industry -- Index

English.

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