PCR detection of microbial pathogens / edited by Konrad Sachse and Joachim Frey.

Includes bibliographical references and index.

Specificity and performance of diagnostic PCR assays / K. Sachse -- Pre-PCR processing of samples / P. Radstrom [and others] -- Critical aspects of standardization of PCR / J. Hoorfar and N. Cook -- PCR technology and applications to zoonotic food-borne bacterial pathogens / P.S. Lubeck and J. Hoorfar -- Detection, identification, and subtyping of actinobacillus pleuropneumoniae / J. Frey -- Identification and differentiation of brucella abortus field and vaccine strains by bass-PCR / D.R. Ewalt and B.J. Bricker -- Isolation of campylobacter and identification by PCR / M.D. Englen, S.R. Ladely and P.J. Fedorka-Cray -- Detection and differentiation of chlamydiae by nested PCR / K. Sachse and H. Hotzel -- Detection of toxigenic clostridia / M.R. Popoff -- PCR-based detection of coxiella burnetii from clinical samples / M. Berri, N. Arricau-Bouvery and A. Rodolakis -- Detection and subtyping of shiga toxin-producing escherichia coli (stec) / P. Gallien -- Detection of listeria monocytogenes using a PCR/DNA probe assay / L. O'Connor -- Detection of leptospira interrogans / J.W. Lester and R.B. LeFebvre -- Detection of pathogenic mycobacteria of veterinary importance / R.A. Skuce [and others] -- Multiplex PCR of avian pathogenic mycoplasmas / M.I. Khan -- Detection and differentiation of ruminant mycoplasmas / H. Hotzel [and others] -- Detection of mycoplasma hyponeumoniae from clinical samples and air / M. Kobisch and J. Frey -- PCR-detection of hemophilus paragallinarum, hemophilus somnus, mannheimia (pasteurella) hemolytica, mannheimia spp., pasteurella trehalosi, and pasteurella multocida / H. Christensen [and others] -- Detection of salmonella SPP / B. Malorny and R. Helmuth -- Detection of toxoplasma gondii / J.M. Wastling and J.G. Mattsson -- PCR-derived methods for the identification of trichinella parasites from animal and human samples / E. Pozio and G. La Rosa -- Detection of pathogenic yersinia enterocolitica by a swab enrichment PCR procedure / R. Knutsson and P. Radstrom -- PCR detection of microbial pathogens. Introduction / K. Sachse and J. Frey.

Print version record.

Because the number of published PCR procedures is already large and rising steadily, there is a need to critically evaluate and eventually standardize its methodology so that laboratory diagnosticians can select optimal techniques for their own work. In PCR Detection of Microbial Pathogens, hands-on laboratory experts present a set of "classic" PCR-based methods for the identification and detection of important animal and food microbial pathogens, including several zoonotic agents. Described in step-by-step detail to ensure successful experimental results, these proven techniques can be precisely applied to a wide variety of microbes, among them Campylobacter spp., chlamydiae, toxigenic clostridia, Escherichia coli (STEC), Listeria monocytogenes, mycoplasmas, salmonellae, and Yersinia enterocolitica. Additional chapters review the specificity and performance of diagnostic PCR analysis, the pre-PCR processing of samples, the critical aspects of standardizing PCR methods, and the general issues involved in using PCR technology for microbial diagnosis. Each article details a validated technique that explains the principles involved, provides stepwise instructions and a list of materials, and includes notes describing problems that may arise, as well as possible alternative procedures. Authoritative and easy-to-use, PCR Detection of Microbial Pathogens offers microbiologists, veterinary diagnosticians, and food analysts powerful state-of-the-art tools designed not only to expand their knowledge of the potential of DNA-based diagnostic methods, but also to help select those techniques best suited to their specific practical needs

English.