Molecular diagnosis of infectious diseases / edited by Jochen Decker, Udo Reischl ; foreword by Christoph Schnorr and Michael Schwörer.

Includes bibliographical references and index.

PART I. PROTEIN-BASED IDENTIFICATION OF ANTIGENS 1. Proteomic Approaches to Antigen Discovery / Karen M. Dobos, John S. Spencer, Ian M. Orme, and John T. Belisle. 2. Immunoproteomics / Alexander Krah and Peter R. Jungblut. 3. Immunoprecipitation and Blotting: The Visualisation of Small Amounts of Antigens Using Antibodies and Lectins / Stephen Thompson. 4. Representational Difference Analysis of cDNA / Lucas D. Bowler. 5. Microarray Data Analysis and Mining / Silvia Saviozzi, Giovanni Iazzetti, Enrico Caserta, Alessandro Guffanti, and Raffaele A. Calogero. 6. Expression Cloning / Michael J. Lodes, Davin C. Dillon, Raymond L. Houghton, and Yasir A.W. Skeiky. PART III. IDENTIFICATION OF EPITOPES AND IMMUNOMODULATORY COMPONENTS. 7. Determination of Epitopes by Mass Spectrometry / Christine Hager-Braun and Kenneth B. Tomer. 8. Identification of T cell Epitopes Using ELISpot and Peptide Pool Arrays / Timothy W. Tobery. 9. Virus-like Particles: A Novel Tool for the Induction and Monitoring of both T-Helper- and Cytotoxic T Lymphocyte Activity / Ludwig Deml, Jens Wild, and Ralf Wagner. 10. Application of Single Cell Cultures of Mouse Splenocytes as an Assay System to Analyze the Immunomodulatory Properties of Bacterial Components / Ludwig Deml, Michael Aigner, Alexander Eckhardt, Jochen Decker, Norbert Lehn, and Wulf Schneider-Brachert. PART IV. EXPRESSION OF RECOMBINANT PROTEINS. 11. High-Throughput Expression and Purification of 6xHis-Tagged Proteins in 96-Well Format / Jason Smith. 12. Production of Antigens in Chlamydomonas reinhardtii: Green Microalgae as a Novel Source of Recombinant Proteins / Markus Fuhrmann. 13. Codon-Optimized Genes that Enable Increased Heterologous Expression in Mammalian Cells and Elicit Efficient Immune Responses in Mice after Vaccination of Naked DNA / Marcus Graf, Ludwig Deml, and Ralf Wagner. PART V. PURIFICATION, MODIFICATION, AND RENATURATION OF RECOMBINANT PROTEINS. 14. Purification and Immunological Characterization of Recombinant Antigens Expressed in Form of Insoluble Aggregates (Inclusion Bodies) / Udo Reischl. 15. Purification of Recombinant Proteins Having High Isoelectric Points / Raffaele A. Calogero and Anna Aulicino. 16. Refolding of Inclusion Body Proteins / Marcus Mayer and Johannes Buchner. 17. Small Molecule-Protein Conjugation Procedures / Stephen Thompson. PART VI. CHARACTERIZATION OF RECOMBINANT PROTEINS. 18. Structural Characterization of Proteins and Peptides / Rainer Deutzmann. 19. Determination of Kinetic Data Using Surface Plasmon Resonance Biosensors / Claudia Hahnefeld, Stephan Drewianka, and Friedrich W. Herberg. 20. Affinity Measurements of Biological Molecules by a Quartz Crystal Microbalance (QCM) Biosensor / Uwe Schaible, Michael Liss, Elke Prohaska, Jochen Decker, Karin Stadtherr, and Hans Wolf. PART VII. EVALUATION OF RECOMBINANT PROTEINS IN IMMUNOLOGICAL TEST SYSTEMS. 21. Solid Supports in Enzyme-Linked Immunosorbent Assay and Other Solid-Phase Immunoassays / John E. Butler. 22. The Design and Preparation of Recombinant Antigens as Diagnostic Reagents in Solid Phase Immunosorbent Assays / Alan Warnes, Anthony R. Fooks, and John R. Stephenson. 23. Basic Problems of Serological Laboratory Diagnosis / Walter Fierz. 24. Molecular Diagnostics Resources on the Internet / Larry Winger. PART VIII. RECOMBINANT RECEPTOR MOLECULES. 25. Cloning Single Chain Antibody Fragments (scFv) from Hybridoma Cells / Lars Toleikis, Olaf Broders, and Stefan Dubel.

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In this second edition of a much-acclaimed classic laboratory manual, Molecular Diagnosis of Infectious Diseases, leading experts from academia and industry describe cutting-edge methods for the protein-based diagnosis of infectious diseases. Explaining the latest developments in genomics, proteomics, bioinformatics, biosensors, high-throughput devices, and recombinant technology, the authors demonstrate the power of these new methodologies in both established and novel procedures by applying them successfully to the identification and characterization of valuable diagnostic markers, immunomodulatory components, epitope mapping, the production and purification of recombinant antigens, and their use in diagnostic reagents in immunological assays. Basic problems of serological laboratory diagnostics and the use of immunoproteomics for discovering target proteins are also addressed. Each readily reproducible protocol is described in step-by-step detail and includes a background introduction, equipment and reagent lists, tips on troubleshooting and avoiding known pitfalls, and extensive references. Comprehensive and up-to-date, Molecular Diagnosis of Infectious Diseases, Second Edition offers novice investigators an easy entry into the field and experienced researchers a broad array of time saving tools that will not only elucidate new principles, but also greatly facilitate the development and successful application of rapid diagnostic methods.

English.