Vascular biology protocols / edited by Nair Sreejayan, Jun Ren.

Includes bibliographical references and index.

Rat carotid artery balloon injury model / David A. Tulis -- Histological and morphometric analyses for rat carotid balloon injury model / David A. Tulis -- Plaque rupture model in mice / Takeshi Sasaki, Kae Nakamura and Masafumi Kuzuya -- Immunostaining of mouse atherosclerotic lesions / Hong Lu, Debra L. Rateri and Alan Daugherty -- Surgical animal model of ventricular hypertrophy / Giuseppe Marano and Alberto U. Ferrari -- Animal models of hypertension / Brett M. Mitchell, Thomas Wallerath and Ulrich Förstermann -- Rat models of cardiac insulin resistance / Sanjoy Ghosh, Brian Rodrigues and Jun Ren -- The Isolated, perfused pseudo-working heart model / Gary J. Grover and Rajni Singh -- Altering and analyzing glucose metabolism in perfused hearts of transgenic mice / Rajakumar V. Donthi and Paul N. Epstein -- Methods in the evaluation of cardiovascular renin angiotensin aldosterone activation and oxidative stress / Camila Manrique -- Detection and quantification of apoptosis in the vasculature / Wendy A. Boivin and David J. Granville -- Endothelial progenitor cell and mesenchymal stem cell isolation, characterization, viral transduction / Keith R. Brunt, Sean R.R. Hall, Christopher A. Ward, and Luis G. Melo -- Assessment of endothelial damage/dysfunction: a focus on circulating endothelial cells / Christopher J. boos, Andrew D. Blann, and Gregory Y.H. Lip -- Evaluation of endothelial function by in vivo microscopy / Rosario Scalia -- Hapotaxis of endothelial cell migration under flow / Steve Hsu, Rahul Thakar, and Song Li -- Isolation, culture, and functional analysis of adult mouse cardiomyocytes / Ronglih Liao and Mohit Jain -- Mechanical measurement of contractile function of isolated ventricular myocytes / Loren E. Wold and Jun Ren -- Studying ischemia and reperfusion in isolated neonatal rat ventricular myocytes using coverslip hypoxia / Kelly R. Pitts and Christopher F. Toombs -- Isolation and functional studies of rat aortic smooth muscle cells / Nair Sreejayan and Xiaoping Yang -- Detection of reactive oxygen species and nitric oxide in vascular cells and tissues / Hua Cai, Sergey Dikalow, Kathy K. Griendling, and David G. Harrison -- Assessment of protein glycoxidation in ventricular tissues / Shi-Yan Li and Jun Ren -- Assessment of PI-3 kinase and Akt in ischemic heart diseases in diabetes / Takashi Matsui and Amy J. Davidoff -- Two-dimensional gel electrophoresis in platelet proteomics research / Ángel García -- Stem cell therapy in the heart and vasculature / Loren E. Wold, Wangde Dai, Joan S. Dow, and Robert A. Kloner.

Print version record.

Over the past decades, the pathogenesis, diagnosis, treatment and prevention of cardiovascular diseases have been benefited significantly from intensive research activities. In order to provide a comprehensive "manual" in a field that has become as broad and deep as cardiovascular medicine, Vascular Biology Protocols covers a wide spectrum of in vivo and in vitro techniques encompassing biochemical, pharmacological and molecular biology disciplines which are currently used to assess vascular disease progression. Each chapter included in this volume focuses on a specific vascular biology technique and describes various applications as well as caveats of these techniques. The protocols included here are described in detail, allowing beginners with little experience in the field of vascular biology to embark on new research projects. Comprehensive and easy-to-use, Vascular Biology Protocols will prove valuable for all those who wish to broaden their knowledge of cardiovascular research tools

English.