Structural proteomics : high-throughput methods / edited by Bostjan Kobe, Mitchell Guss, Thomas Huber.

Includes bibliographical references and index.

Target selection for structural genomics : an overview / Russell L. Marsden and Christine A. Orengo -- A general target selection method for crystallographic proteomics / Gautier Robin [and others] -- Target selection : triage in the structural genomics battlefield / James Raftery -- Data management in structural genomics : an overview / Sabrina Haquin [and others] -- Data deposition and annotation at the worldwide protein data bank / Shuchismita Dutta [and others] -- Prediction in protein disorder / Zsuzsanna Dosztányi and Peter Tompa -- Protein domain prediction / Helgi Ingolfsson and Golan Yona -- Protein structure modeling with MODELLER / Narayanan Eswar [and others] -- High throughput cloning with restriction enzymes / Volker Sievert, Asgar Ergin, and Konrad Bussow -- Automated recombinant protein expression screening in Escherichia coli / Didier Busso [and others] -- From no expression to high-level soluble expression in Escherichi coli by screening a library of the target proteins with randomized N-termini / Kyoung Hoon Kim [and others] -- Application of high-throughput methodologies to the expression of recombinant proteins in E. coli / Yoav Peleg and Tamar Unger -- A high throughput platform for eukaryotic genes / Yunjia Chen [and others] -- High throughput production of recombinant human proteins for crystallography / Opher Gileadi [and others] -- Assembly of protein complexes by coexpression in prokaryotic and eukaryotic hosts : an overview / Anastassis Perrakis and Christophe Romier -- Cell-free protein synthesis for analysis by NMR spectroscopy / Margit A. Apponyi [and others] -- A medium or high throughput protein refolding assay / Nathan P. Cowieson [and others] -- Structural proteomics of membrane proteins : a survey of published techniques and design of a rational high throughput strategy / Melissa Swope Willis and Christopher M. Koth -- Methods for protein characterization by mass spectrometry, thermal shift (ThermoFluor) assay, and multiangle or static light scattering / Joanne E. Nettleship [and others] -- High throughput methods for analyzing transition metals in proteins on a microgram scale / Anelia Atanassova, Martin Högbom, and Deborah B. Zamble -- High throughput screening of purified proteins for enzymatic activity / Michael Proudfoot [and others] -- Strategies for improving crystallization success rates / Rebecca Page -- Protein crystallization in restricted geometry : advancing old ideas for modern times in structural proteomics / Joseph D. Ng, Raymond C. Stevens, and Peter Kuhn -- Fluorescence approaches to growing macromolecule crystals / Marc Pusey, Elizabeth Forsythe, and Aniruddha Achari -- Efficient macromoleular crystallization using microfluidics and randomized design of screening reagents / Andrew P. May and Brent W. Segelke -- Increasing protein crystallization screening success with heterogeneous nucleating agents / Anil S. Thakur [and others] -- High throughput pH optimization of protein crystallization / Ran Meged, Orly Dym, and Joel L. Sussman -- Automated structure solution with the PHENIX suite / Peter H. Zwart [and others] -- NMR screening for rapid protein characterization in structural proteomics / Justine M. Hill -- Microcoil NMR spectroscopy : a novel tool for biological high throughput NMR spectroscopy / Russell E. Hopson and Wolfgang Peti -- Protein structure determination using a combination of cross-linking, mass spectrometry, and molecular modeling / Dmitri Mouradov [and others] -- Structural genomics of minimal organisms : pipeline and results / Sung-Hou Kim [and others] -- Structural genomics of pathogenic protozoa : an overview / Erkang Fan [and others] -- High throughput crystallography at SGC Toronto : an overview / Alexey Bochkarev and Wolfram Tempel -- The structural biology and genomics platform in Strasbourg : an overview / Didier Busso, Jean-Claude Thierry, and Dino Moras -- Bacterial structural genomics initiative : overview of methods and technologies applied to the process of structure determination / Miroslaw Cygler [and others] -- High-throughput protein production and crystallization at NYSGXRC / Michael J. Sauder [and others] -- Overview of the pipeline for structural and functional characterization of macrophage proteins at the University of Queensland / Weining Meng [and others] -- Structural genomics of the bacterial mobile metagenome : an overview / Andrew Robinson [and others].

Annotation The objective of this volume is to provide readers with a current view of all aspects of the 'pipeline' that takes protein targets to structures and how these have been optimised. This volume includes chapters describing, in-depth, the individual steps in the Structural Genomics pipeline, as well as less detailed overviews of individual Structural Genomics initiatives. It is the first book of protocols to cover techniques in a new and emerging field.

Print version record.

English.