Molecular beacons : signalling nucleic acid probes, methods, and protocols / edited by Andreas Marx and Oliver Seitz.

Includes bibliographical references and index.

Basic principles of fluorescence and energy transfer / Larry E. Morrison -- Multiplex detection of mutations / David S. Perlin, Sergey Balashov, and Steven Park -- Detection of salmonella species in foodstuffs / Arvind A. Bhagwat [and others] -- Identification of homozygous transgenic mice by genomic real-time PCR / Gunter Schmidtke and Marcus Groettrup -- Quantitative analysis of gene expression relative to 18S rRNA in carcinoma samples using the LightCycler instrument and a SYBR greenI-based assay : determining FAT10 mRNA levels in hepatocellular carcinoma / Sebastian Lukasiak [and others] -- Absolute quantitation of feline leukemia virus proviral DNA and viral RNA loads by TaqMan real-time PCR and RT-PCR / Valentino Cattori and Regina Hofmann-Lehmann -- High-throughput real-time PCR / Thomas D. Schmittgen, Eun Joo Lee, and Jinmai Jiang -- Design and use of scorpions fluorescent signalling molecules / Rachael Carters [and others] -- Hybridization probe pairs and single-labeled probes : an alternative approach for genotyping and quantification / Thomas Froehlich and Oliver Geulen -- EasyBeacons for the detection of methylation status of single CpG duplets / Ulf B. Christensen -- Quenched autoligation probes / Adam P. Silverman, Hiroshi Abe, and Eric T. Kool -- HyBeacon probes for rapid DNA sequence detection and allele discrimination / David J. French [and others] -- FIT-probes in real-time PCR / Elke Socher and Oliver Seitz -- SNP genotyping by unlabeled probe melting analysis / Maria Erali, Robert Palais, and Carl Wittwer -- Molecular beacons for protein-DNA interaction studies / Jun Li [and others] -- DNA polymerase profiling / D. Summerer -- Modular reporter hairpin ribozymes for analyzing molecular interactions / S. Hani Najafi-Shoushtari and Michael Famulok -- Screening of molecular interactions using reporter hammerhead ribozymes / Jörg S. Hartig and Michael Famulok.

Print version record.

Significant DNA sequence data has been made available by several important genome projects. The allocation of functions to a mere string of the four letters in the code of life plays a major if not predominant role of ongoing and future research. Guided by experts in the field, Molecular Beacons: Signalling Nucleic Acid Probes, Methods, and Protocols, provides easy-to-follow experiments, commences with an introduction to the basic principles of fluorescence, and shows a diverse set of instructive examples, from probe design to applications in clinical settings, molecular beacon technology, the identification of transgenic mice, along with the potential of realtime PCR. While originally being used for fluorogenic nucleic acid probes with a stem-loop structure, molecular beacon now refers to a whole family of fluorescence-up probes. Molecular Beacons: Signalling Nucleic Acid Probes, Methods, and Protocols summarizes all the latest findings and explores powerful techniques designed for geneticist, biotechnologists, molecular and cellular biologists, researchers and developers.

English.