Alcohol : methods and protocols / Laura E. Nagy, editor.

Includes bibliographical references and index.

Animal models of acute and chronic ethanol exposure. Acute models of ethanol exposure to mice / Timothy P. Plackett and Elizabeth J. Kovacs. A voluntary oral-feeding rat model for pathological alcoholic liver injury / George L. Tipoe [and others]. Intragastric ethanol infusion model in rodents / Hide Tsukamoto, Hasmik Mkrtchyan, and Alla Dynnyk. A practical method of chronic ethanol administration in mice / Ruth A. Coleman [and others] -- Animal models of ethanol exposure during development:. Analysis of ethanol developmental toxicity in zebrafish / Robert L. Tanguay and Mark J. Reimers. The avian embryo in fetal alcohol research / Susan M. Smith. Artificial rearing / Hector D. Dominguez and Jennifer D. Thomas. Intragastric intubation of alcohol during the perinatal period / Sandra J. Kelly and Charles R. Lawrence -- Cell culture approaches to studying ethanol exposure. Human monocytes, macrophages, and dendritic cells : alcohol treatment methods / Gyongyi Szabo and Pranoti Mandrekar -- Generation and use of primary rat cultures for studies of the effects of ethanol / Amanda Lindke, Barbara Tremper-Wells, and Michael W. Miller. Development and properties of HepG2 cells that constitutively express CYP2E1 / Defeng Wu and Arthur I. Cederbaum. Modeling the impact of alcohol on cortical development in a dish : strategies from mapping neural stem cell fate / Rajesh C. Miranda [and others] -- Regulation of specific organ systems in response to ethanol. Acetaldehyde-induced barrier disruption and paracellular permeability in Caco-2 cell monolayer / R.K. Rao. Alcohol-induced oxidative stress in the liver : in vivo measurements / Gavin E. Arteel. Isolation of Kupffer cells from rats fed chronic ethanol / Megan R. McMullen, Michele T. Pritchard, and Laura E. Nagy. Dendritic cells in chronic in vivo ethanol exposure models / Kevin L. Legge and Annette J. Schlueter. Formation and immunological properties of aldehyde-derived protein adducts following alcohol consumption / Geoffrey M. Thiele, Lynell W. Klassen, and Dean J. Tuma. Assessment of natural killer (NK) and NKT cells in murine spleens and livers / Michael R. Shey and Zuhair K. Ballas. Polyclonal and antigen-specific responses of T cells and T cell subsets / Betty M. Young [and others]. B-cell studies in chronic ethanol mice / Shilpi Verma [and others]. Histological analysis of bone / Urszula T. Iwaniec, Thomas J. Wronski, and Russell T. Turner. Assessing effects of alcohol consumption on protein synthesis in striated muscles / Thomas C. Vary and Charles H. Lang. Methods to investigate the effects of chronic ethanol on adipocytes / Becky M. Sebastian [and others] -- Proteomic and genomic approaches to study response to ethanol. Proteomic approaches to identify and characterize alterations to the mitochondrial proteome in alcoholic liver disease / Shannon M. Bailey [and others]. Alcoholic liver disease and the mitochondrial ribosome : methods of analysis / Alan Cahill and Peter Sykora. Microarray analysis of ethanol-induced changes in gene expression / Robnet T. Kerns and Michael F. Miles.

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Many unexplored complex cellular and organismal adaptations occur in response to the stress of alcohol exposure, and its contribution to the development of chronic diseases, such as osteoporosis, heart disease and diabetes, is particularly relevant today, given the increased incidence of these diseases in our aging population. In Alcohol: Methods and Protocols, the pleiotropic effects of ethanol in animal and cell culture models are rigorously examined through a collection of detailed procedures written by experts in the field. Sections present clearly defined models of ethanol exposure, recent advances in the development of specific methodologies to mimic the impact of ethanol metabolism in cultured cells, and methodologies to investigate a variety of cells and tissues that are known to be disrupted by ethanol, amongst other topics. As part of the successful Methods in Molecular Biology series, each chapter provides step-by-step laboratory protocols, lists of the necessary materials, and a Notes section with tips on troubleshooting and avoiding known pitfalls. Comprehensive and highly specific, Alcohol: Methods and Protocols strives to further spur investigators to delve into the complex and fascinating processes of the adaptive and maladaptive responses humans have developed to the consumption of alcohol.

English.