Tumor biomarker discovery : methods and protocols / edited by Michael A. Tainsky.

Includes bibliographical references and index.

Identification of tumor-associated antigens as diagnostic and predictive biomarkers in cancer / Jian-Ying Zhang, Kok Sun Looi, and Eng M. Tan -- Autoantibodies against cancer antigens / Sacha Gnjatic, Lloyd J. Old, and Yao-Tseng Chen -- Discovery of antibody biomarkers using protein microarrays of tumor antigens cloned in high throughput / Madhumita Chatterjee, Jerzy Wojciechowski, and Michael A. Tainsky -- Analysis of glycans on serum proteins using antibody microarrays / Songming Chen and Brian B. Haab -- Glycoproteomic analysis by two-dimensional electrophoresis / Mary Ann Comunale and Anand Mehta -- All-liquid separations, protein microarrays, and mass spectrometry to interrogate serum proteomes : an application to serum glycoproteomics / Tasneem H. Patwa [and others] -- Reverse-phase protein microarrays for theranostics and patient tailored therapy / Virginia Espina, Lance A. Liotta, and Emanuel F. Petricoin III -- Serum proteomics using mass spectrometry / Brian L. Hood [and others] -- Hormones as biomarkers : practical guide to utilizing Luminex technologies for biomarker research / Faina Linkov, Zoya Yurkovetsky, and Anna Lokshin -- High-throughput analysis of serum antigens using sandwich ELISAs on microarrays / Shannon L. Servoss [and others] -- Tissue microarrays as a tool in the discovery and validation of tumor markers / Stephen M. Hewitt -- Quantitative, fluorescence-based in situ assessment of protein expression / Christopher B. Moeder [and others] -- Tumor marker discovery by expression profiling RNA from formalin-fixed paraffin-embedded tissues / Maureen T. Cronin [and others] -- High-throughput mutation screening using a single amplification condition / Lijia Shi and John E. Landers -- DNA sequencing of cancer-related genes for biomarker discovery / Lisa M. Farwell and Victoria A. Joshi -- Analysis of loss of heterozygosity in circulating DNA / Takeshi Nakamura [and others] -- Pharmacogenomics / Silong Zhong and Marjorie Romkes -- Study designs in genetic epidemiology / Michele L. Cote -- Developing classifiers for the detection of cancer using multianalytes / Adi Laurentiu Tarca, Sorin Draghici, and Roberto Romero -- Metabolomics of cancer / Natalie J. Serkova and Kristine Glunde -- MRI and MRS of human brain tumors / Bob L. Hou and Jiani Hu -- Magnetic resonance sprctroscopy of living tissues / Natalie J. Serkova, Kendra M. Hasebroock, and Susan L. Kraft.

Print version record.

Through a variety of mechanisms, cancer cells provide the biomarker material that can lead to their own detection, which then grants clinicians the opportunity for non-invasive body fluid and tissue analysis able to indicate the presence of tumors or the level of tumor burden. In Tumor Biomarker Discovery: Methods and Protocols, experts in the field present detailed chapters focused on the use and development of panels of biomarker analytes while covering subjects such as nucleic acids and protein-based technologies, metabolic profiling by analytic means or spectroscopy, as well as study designs for biomarker discovery and validation. Written in the highly successful Methods in Molecular Biology series format, chapters include brief introductions to the topics, lists of the necessary materials or reagents, step-by-step laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Cutting-edge and authoritative, Tumor Biomarker Discovery: Methods and Protocols is an ideal guide to the technologies that can be used to discover and validate tumor biomarker panels suitable for the presymptomatic early detection of cancer.

English.