Host-pathogen interactions : methods and protocols / edited by Steffen Rupp and Kai Sohn.

Includes bibliographical references and index.

Introduction : bacterial pathogens / Christof R. Hauck -- Transcriptome analyses in the interaction of Neisseria meningitidis with mammalian host cells / Alexandra Schubert-Unkmeir [and others] -- Surface-exposed adherence molecules of Streptococcus pneumoniae / Sven Hammerschmidt -- Screening of virulence traits in Legionella pneumophila and analysis of the host susceptibility to infection by using the Dictyostelium host model system / Olaga Shevchuk and Michael Steinert -- Characterizing host receptor recognition by individual bacterial pathogens / Katharina Kuespert and Christof R. Hauck -- Introduction : fungal pathogens / Steffen Rupp -- Isolation and purification of antigenic components of Cryptococcus / Karen L. Wozniak and Stuart M. Levitz -- A method for examining glycans surface expression of yeasts by flow cytometry / Maria Martínez-Esparza [and others] -- Human epithelial model systems for the study of Candida infections in vitro : part I. Adhesion to epithelial models / Kai Sohn and Steffen Rupp -- Human epithelial model systems for the study of Candida infections in vitro : part II. Histologic methods for studying fungal invasion / Rosa Hernandez and Steffen Rupp -- In vitro systems for studying the interaction of fungal pathogens with primary cells from the mammalian innate immune system / Christelle Bourgeois [and others] -- Experimental infection of rodent mammals for fungal virulence testing / Anja Schweizer and Klaus Schröppel -- Real-time and semiquantitative RT-PCR methods to analyze gene expression patterns during Aspergillus-host interactions / Jin Woo Bok, Nancy P. Keller, and Dimitrios I. Tsitsigiannis -- Antibody-based strategy to identify Candida albicans genes expresssed during infections / Cornelius J. Clancy, Shaoji Cheng, and M. Hong Nguyen -- Identification of the Candida albicans immunome during systemic infection by mass spectrometry / Aida Pitarch, César Nombela, and Concha Gil -- Introduction : parasites / Klaus Brehm and Carsten G.K. Lüder -- Axemic in vitro cultivation of Echinococcus multilocularis metacestode vesicles and the generation of primary cell cultures / Markus Spiliotis and Klaus Brehm -- Culture of exoerythrocytic stages of the malaria parasites Plasmodium falciparum and Plasmodium vivax / Liwang Cui [and others] -- Modulation of caspase activation by Toxoplasma gondii / Diana Hippe [and others] -- Introduction : host responses / Martin Schaller, Günther Weindl, and Bernhard Hube -- Fungal and bacterial killing by neutrophils / David Ermert, Arturo Zychlinsky, and Constantin Urban -- Endothelial cell stimulation by Candida albicans / Quynh T. Phan and Scott G. Filler -- Models of oral and vaginal candidiasis based on in vitro reconstituted human epithelia for the study of host-pathogen interactions / Martin Schaller and Günther Weindl -- Phagocytosis of Candida albicans by RNAi-treated Drosophila S2 cells / Shannon L. Stroschein-Stevenson [and others] -- Oral mucosal cell response to Candida albicans in transgenic mice expressing HIV-1 / Louis de Repentigny [and others] -- Proteomic profiling of serologic response to Candida albicans during host-commensal and host-pathogen interactions / Aida Pitarch, César Nombela, and Concha Gil.

In recent decades, infectious diseases, once believed to be fairly contained, have become a vital, resurgent field of research. In Host-Pathogen Interactions: Methods and Protocols, top experts examine the relationship between the host and the pathogen, crucial in the outcome of an infection and the establishment of disease or asymptomatic, commensal colonization by organisms. The step-by-step laboratory methods and protocols of this volume study host-pathogen interaction, with a focus on fungal, bacterial and parasitic pathogens, at a molecular level in order to reveal the mechanisms of infection and to identify the vulnerabilities of the pathogen of interest. The chapters feature brief subject introductions, lists of the necessary materials and reagents, and tips on troubleshooting and avoiding known pitfalls.