Lipid signaling protocols / Banafshé Larijani, editor ; Rudiger Woscholski, editor ; Colin A. Rosser, editor.

Includes bibliographical references and index.

Eicosanoids : generation and detection in mammalian cells / Valerie B. O'Donnell, Ben Maskrey, and Graham W. Taylor -- Lipidomic analysis of phospholipids and related structures by liquid chromatography-mass spectrometry / Trevor R. Pettitt -- Measurement of polyphosphoinositides in cultured mammalian cells / Frank T. Cooke -- Inositol lipid-dependent functions in Saccharomyces cerevisiae : analysis of phosphatidylinositol phosphates / Stephen K. Dove and Robert H. Michell -- Methods for the determination of the masss of nuclear PtdIns4P, PtdIns5P, and PtdIns(4,5)P<<SUBSCRIPT 2>> / David R. Jones [and others] -- Lipid quantification and structure determination of nuclear envelope precursor membranes in the sea urchin / Marie Garnier-Lhomme [and others] -- Solution and solid-state NMR of lipids / Axelle Grelard [and others] -- Spatial localization of PtdInsP<<SUBSCRIPT 2>> in phase-separated giant unilamellar vesicles with a fluorescent PLC-delta 1 PH domain / Xavier Mulet [and others] -- Studying cell-to-cell interactions : an easy method of tethering ligands on artificial membranes / Sebastian J. Fleire and Facundo D. Batista -- Ceramide-induced transbilayer (flip-flop) lipid movement in membranes / F.-Xabier Contreras [and others] -- Effects of sterols on the development and aging of Caenorhabditis elegans / Eun-Young Lee [and others] -- Proteomic analysis of the sterol-mediated signaling pathway in Caenorhabditis elegans / Byung-Kwon Choi [and others] -- Sudying the subcellular localization and DNA-binding activity of FoxO transcription factors, downstream effectors of P13K/Akt / Abdelkader Essafi [and others] -- Measurement of PTEN activity in vivo by imaging phosphorylated Akt / Erika Rosivatz and Rudiger Woscholski -- Analysis of phosphatidylinositol 3,4,5 trisphosphate 5-phosphatase activity by in vitro and in vivo assays / Lisa M. Ooms [and others] -- Measuring phospholipase D activity in insulin-secreting pancreatic [beta]-cells and insulin-responsive muscle cells and adipocytes / Rosanna Cazzolli [and others] -- Protein kinase C as an effector of lipid-derived second messengers / Marie-Hélène Paclet [and others] -- Detection of myotubularin phosphatases activity on phosphoinositides in vitro and ex vivo / Holger Maria Rohde [and others] -- Quantification of multiple phosphatidylinositol 4-kinase isozyme activities in cell extracts / Mark G. Waugh, Shane Minogue, and J. Justin Hsuan -- Phospholipid-interacting proteins by solution-state NMR spectroscopy / Keiichiro Kami, Sundaresan Rajesh, and Michael Overduin -- Revealing signaling in single cells by single- and two-photon fluorescence lifetime imaging microscopy / Damien Alcor, Veronique Calleja, and Banafshé Larijani -- Phosphoinositide (PI) 3-kinase assays / Michael J. Fry -- Measurement of phosphatidylinositol and phosphatidylcholine binding and transfer activity of the lipid transport Protein PITP / Shamshad Cockcroft -- In vitro reconstitution of activation of PLC[epsilon] by Ras and Rho GTPases / Natalia Lamuño Gandarillas [and others] -- Assaying endogenous phosphatidylinositol-4-phosphate 5-kinase (PIP5K) activities / Jonathon R. Halstead [and others] -- Preparation of membrane rafts / Mark G. Waugh and J. Justin Hsuan.

Print version record.

Lipid Signaling Protocols assembles in a single volume the various tools and methodologies needed by the interested investigator to unravel lipid dependent signaling and cell function. Divided into two convenient sections, the volume begins by summarizing the physical properties of hydrophobic metabolites as well as the physical methodologies used for their analysis, which leads to the second section and its selection of biological methods, focused around the most relevant lipids, their corresponding metabolizing enzymes and the recognition proteins. Following the highly successful Methods in Molecular Biology series format, the chapters provide readily reproducible laboratory protocols, lists of necessary materials and reagents, and the tips on troubleshooting and avoiding known pitfalls. Contributed to by top researchers in the field, Lipid Signaling Protocols is an essential resource for both experienced and novice researchers who desire a better understanding of the application of physical methodologies in the context of lipid signaling and lipid metabolism in cell biology.

English.