Xenotransplantation : methods and protocols / edited by Cristina Costa, Rafael Manez.

Xenotransplantation : an overview of the field / Veronica Tisato and Emanuele Cozzi -- Cloning and expression analyses of pig genes / Mireia Uribe-Herranz and Cristina Costa -- Basic analyses of proteins of interest for xenotransplantation / Mireia Uribe-Herranz and Cristina Costa -- Studies on carbohydrate xenoantigens / Dale Christiansen [and others] -- Xenoantibodies and complement activity determinations in pig-to-primate xenotransplantation / Nieves Domenech -- Studies on coagulation incompatibilities for xenotransplantation / Cristiana Bulato, Claudia Radu, and Paolo Simioni -- Cellular studies for in vitro modeling of xenogeneic immune responses / Roberta Sommaggio, Magdiel Perez-Cruz, and Cristina Costa -- Production of transgenic and knockout pigs by somatic cell nuclear transfer / Angelica M. Giraldo, Suyapa Ball, and Kenneth R. Bondioli -- Small animal models of xenotransplantation / Hao Wang -- Heart xenotransplantation in primate models / Johannes Postrach [and others] -- Xenogeneic lung transplantation models / Lars Burdorf, Agnes M. Azimzadeh, and Richard N. Pierson, III -- Thymic transplantation in pig-to-nonhuman primates for the induction of tolerance across xenogeneic barriers / Kazuhiko Yamada and Joseph Scalea -- Isolation of porcine pancreatic islets for xenotransplantation / Karin Ulrichs [and others] -- Pig neural cells derived from foetal mesencephalon as cell source for intracerebral xenotransplantation / Xavier Leveque [and others] -- Hepatocyte xenotransplantation / Katia R.F. Lima-Quaresma [and others] -- In vitro repair model of focal articular cartilage defects in humans / Diaz Prado SM, Fuentes-Boquete IM, and Blanco FJ -- Potential zoonotic infection of porcine endogenous retrovirus in xenotransplantation / Giada Mattiuzzo, Yasuhiro Takeuchi, and Linda Scobie -- Ethical and regulatory issues for clinical trials in xenotransplantation / Jorge Guerra Gonzalez -- Some ethical, social, and legal considerations of xenotransplantation / Maria Jorqui Azofra and Carlos Maria Romeo Casabona.

Includes bibliographical references and index.

Print version record.

Despite many technological challenges faced by the xenotransplantation field, many major advances have been made in the last two decades. The field seeks to overcome the limitations and difficulties in organ procurement, which also apply to human cells and tissues, and facilitate the development of new therapies based on cell and engineered-tissue. Xenogeneic cells are simpler than solid organs and seem to pose less hurdles to attain long-term graft survival. In, Xenotransplantation: Methods and Protocols expert researchers study characterizations of xenogeneic interactions at the cellular and molecular levels and describe the use of relevant small-animal and pig-to-primate models. Related ethical and legal considerations are also covered. Written in the highly successful Methods in Molecular Biology series format, the chapters include the kind of detailed description and implementation advice that is crucial for getting optimal results in the laboratory. Thorough and intuitive, Xenotransplantation: Methods and Protocols aids scientists in continuing to study xenotransplantation and its multiple aspects.

English.