Cell imaging techniques : methods and protocols / edited by Douglas J. Taatjes, Jurgen Roth.

Digital images are data : and should be treated as such / Douglas W. Cromey -- Epi-fluorescence microscopy / Donna J. Webb and Claire M. Brown -- Live-cell migration and adhesion turnover assays / J. Lacoste, K. Young, and Claire M. Brown -- Multifluorescence confocal microscopy : application for a quantitative analysis of hemostatic proteins in human venous valves / Winifred E. Trotman, Douglas J. Taatjes, and Edwin G. Bovill -- Colocalization analysis in fluorescence microscopy / Jeremy Adler and Ingela Parmryd -- Time-lapse imaging assay to study nuclear envelope breakdown / Sunita S. Shankaran, Douglas R. Mackay, and Katharine S. Ullman -- Light sheet microscopy in cell biology / Raju Tomer, Khaled Khairy, and Philipp J. Keller -- Image-based high-throughput screening for inhibitors of angiogenesis / Lasse Evensen, Wolfgang Link, and James B. Lorens -- Intravital microscopy to image membrane trafficking in live rats / Andrius Masedunskas [and others] -- Imaging non-fluorescent nanoparticles in living cells with wavelength-dependent differential interference contrast microscopy and planar illumination microscopy / Wei Sun, Lehui Xiao, and Ning Fang -- Laser scanning cytometry : principles and applications : an update / Piotr Pozarowski, Elena Holden, and Zbigniew Darzynkiewicz -- Laser capture microdissection for protein and nanostring RNA Analysis / Yelena Golubeva [and others] -- Viewing dynamic interactions of proteins and a model lipid membrane with atomic force microscopy / Anthony S. Quinn [and others] -- Mica functionalization for imaging of DNA and protein-DNA complexes with atomic force microscopy / Luda S. Shlyakhtenko, Alexander A. Gall, and Yuri L. Lyubchenko -- Measuring the elastic properties of living cells with atomic force microscopy indentation / Joanna L. MacKay and Sanjay Kumar -- Atomic force microscopy functional imaging on vascular endothelial cells / Lilia A. Chtcheglova and Peter Hinterdorfer -- Porosome : the secretory nanomachine in cells / Bhanu P. Jena -- Stereology and morphometry of lung tissue / Christian Muhlfeld, Lars Knudsen, and Matthias Ochs -- Novel combined imaging/morphometrical method for the analysis of human sural nerve biopsies for clinical diagnosis / Michele A. vonTurkovich [and others] -- Correlative light-electron microscopy as a tool to study in vivo dynamics and ultrastructure of intracellular structures / Elena V. Polishchuk, Roman S. Polishchuk, and Alberto Luini -- Photooxidation technology for correlative light and electron microscopy / Claudia Meisslitzer-Ruppitsch [and others] -- Electron microscopy of endocytic pathways / Carmen Ranftler [and others] -- Morphological analysis of autophagy / Keisuke Tabata [and others] -- Cytochemical detection of peroxisomes and mitochondria / Nina A. Bonekamp [and others] -- Histochemical detection of lipid droplets in cultured cells / Michitaka Suzuki, Yuki Shinohara, and Toyoshi Fujimoto -- Environmental scanning electron microscopy in cell biology / J.E. McGregor [and others] -- Environmental scanning electron microscopy gold immunolabeling in cell biology / Francesco Rosso, Ferdinando Papale, and Alfonso Barbarisi -- High-pressure freezing for scanning transmission electron tomography analysis of cellular organelles / Paul Walther, Eberhard Schmid, and Katharina Hohn -- MALDI imaging mass spectrometry for direct tissue analysis / Stephan Meding and Axel Walch.

Includes bibliographical references and index.

Cell Imaging is rapidly evolving as new technologies and new imaging advances continue to be introduced. In the second edition of Cell Imaging Techniques: Methods and Protocols expands upon the previous editions with current techniques that includes confocal microscopy, transmission electron microscopy, atomic force microscopy, and laser microdissection. With new chapters covering colocalization analysis of fluorescent probes, correlative light and electron microscopy, environmental scanning electron microscopy, light sheet microscopy, intravital microscopy, high throughput microscopy, and stereological techniques. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls Authoritative and cutting-edge, Cell Imaging Techniques: Methods and Protocols, Second Edition is an easily accessible volume of protocols to be used with a variety of imaging-based equipment likely available in a core imaging facility.

English.