Nanoimaging : methods and protocols / edited by Alioscka A. Sousa, Michael J. Kruhlak.

Includes bibliographical references and index.

Introduction : nanoimaging techniques in biology / Alioscka A. Sousa and Michael J. Kruhlak -- Live-cell imaging of vesicle trafficking and divalent metal ions by total internal reflection fluorescence (TIRF) microscopy / Merewyn K. Loder, Takashi Tsuboi, and Guy A. Rutter -- 4Pi microscopy / Roman Schmidt, Johann Engelhardt, and Marion Lang --- Fluorescence in situ hybridization applications for super-resolution 3D structured illumination microscopy / Yolanda Markaki [and others] -- Two-color STED imaging of synapses in living brain slices / Jan Tonnesen and U. Valentin Nagerl -- Super-resolution imaging by localization microscopy / Dylan M. Owen [and others] -- High-content super-resolution imaging of live cell by uPAINT / Gregory Giannone [and others] -- Super-resolution fluorescence imaging with blink microscopy / Christian Steinhauer, Michelle S. Itano, and Philip Tinnefeld -- Photoswitchable fluorophores for single-molecule localization microscopy / Kieran Finan, Benjamin Flottmann, and Mike Heilemann -- Single-molecule tracking of mRNA in living cells / Mai Yamagishi, Yoshitaka Shirasaki, and Takashi Funatsu -- Semiautomatic, high-throughput, high-resolution protocol for three-dimensional reconstruction of single particles in electron microscopy / Carlos Oscar Sorzano [and others] -- Mass mapping of amyloid fibrils in the electron microscope using STEM imaging / Alioscka A. Sousa and Richard D. Leapman -- Elemental mapping by electron energy loss spectroscopy in biology / Maria A. Aronova and Richard D. Leapman -- Cellular nanoimaging by cryo electron tomography / Roman I. Koning and Abraham J. Koster -- Large-volume reconstruction of brain tissue from high-resolution serial section images acquired by SEM-based scanning transmission electron microscopy / Masaaki Kuwajima, John M. Mendenhall, and Kristen M. Harris -- 3D imaging of cells and tissues by focused ion beam/ scanning electron microscopy (FIB/SEM) / Damjana Drobne -- Preparation of gold nanocluster bioconjugates for electron microscopy / Christine L. Heinecke and Christopher J. Ackerson -- Atomic force microscopy imaging of macromolecular complexes / Sergio Santos, Daniel Billingsley, and Neil Thomson -- Imaging of transmembrane proteins directly incorporated within supported lipid bilayers using atomic force microscopy / Daniel Levy and Pierre-Emmanuel Milhiet -- Functional AFM imaging of cellular membranes using functionalized tips / Lilia A. Chtcheglova and Peter Hinterdorfer -- Near-field scanning optical microscopy for high-resolution membrane studies / Heath A. Huckabay [and others] -- Correlative fluorescence and EFTEM imaging of the organized components of the mammalian nucleus / Michael J. Kruhlak -- High data output method for 3-D correlative light-electron microscopy using ultrathin cryosections / Katia Cortese [and others] -- Correlative optical and scanning probe microscopies for mapping interactions at membranes / Christopher M. Yip -- Nanoimaging cells using soft x-ray tomography / Dilworth Y. Parkinson [and others] -- Secondary ion mass spectrometry imaging of biological membranes at high spatial resolution / Haley A. Klitzing, Peter K. Weber, and Mary L. Kraft.o.

Print version record.

For more than a century, microscopy has been a centerpiece of extraordinary discoveries in biology. Along the way, remarkable imaging tools have been developed allowing scientists to dissect the complexity of cellular processes at the nano length molecular scales. Nanoimaging: Methods and Protocols presents a diverse collection of microscopy techniques and methodologies that provides guidance to successfully image cellular molecular complexes at nanometer spatial resolution. The book's four parts cover: (1) light microscopy techniques with a special emphasis on methods that go beyond the classic diffraction-limited imaging; (2) electron microscopy techniques for high-resolution imaging of molecules, cells and tissues, in both two and three dimensions; (3) scanning probe microscopy techniques for imaging and probing macromolecular complexes and membrane surface topography; and (4) complementary techniques on correlative microscopy, soft x-ray tomography and secondary ion mass spectrometry imaging. Written in the successful format of the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Nanoimaging: Methods and Protocols highlights many of the most exciting possibilities in microscopy for the investigation of biological structures at the nano length molecular scales.

English.