Peroxisome proliferator-activated receptors (PPARs) : methods and protocols / edited by Mostafa Z. Badr, Jihan Youssef.

880-01 PPARs : history and advances / Jihan Youssef and Mostafa Z. Badr -- PPAR-alpha cloning, expression, and characterization / Suong N.T. Ngo and Ross A. McKinnon -- PPARGC1A and PPARG genotyping : beginner's guide to genotyping with unlabeled probes / Viera Habalova and Jozef Zidzik -- Generation of an inducible, cardiomyocyte-specific transgenic mouse model with PPAR b/d overexpression / Teayoun Kim [and others] -- Specific knockdown of PPARd gene in colon cancer cells by lentivirus-mediated RNA Interfering / Lie Yang, Zong-Guang Zhou, and Xiao-Feng Sun -- Dominant-negative and knockdown approaches to studying PPAR activity / Sudhir Singh and Robert G. Bennett -- Producing PPARgamma2 knockdown in mouse liver / Tomomi Yamazaki and Osamu Ezaki -- Adipose tissue-specific PPARg gene targeting / Weimin He -- Site-directed mutagenesis to study the role of specific amino acids in the ligand binding domain of PPARs / Nico Mitro ... [e tal.] -- PPAR SUMOylation : some useful experimental tips / Benoit Pourcet, Bart Staels, and Corine Glineur -- Analyzing phosphorylation-dependent regulation of subcellular localization and transcriptional activity of transcriptional coactivator NT-PGC-1a / Ji Suk Chang and Thomas W. Gettys -- In vivo studies of PPAR-chromatin interactions : chromatin immunoprecipitation for single-locus and genomewide analyses / Till Adhikary and Rolf Muller -- FISH analysis using PPAR g-specific probes for detection of PAX8-PPAR g translocation in follicular thyroid neoplasms / Noor Akmal Syed Husain Sharifah, Zubaidah Zakaria, and Wai Kit Chia -- Immunohistochemical techniques to identify and localize proteins of interest in paraffin embedded tissue sections / Carolyn M. Komar and Mary Jane Long -- Determination of PPAR expression by western blot / Aziz Guellich and Catherine Coirault -- Fluorescence resonance energy transfer techniques to study ligand-mediated interactions of PPARs with coregulators / Nico Mitro, Cristina Godio, and Maurizio Crestani -- Estimation of the PPARa agonism of fibrates by a combined MM-docking approach / Fabio Lannutti, Alessandro Marrone, and Nazzareno Re -- Combined biophysical and cell-based approaches for the assessment of ligand binding to PPARg / Silvia Zorrilla and Dolores Perez-Sala -- Exploring PPAR modulation in experimental mice / Anna Rull [and others] -- Induction of adipogenic differentiation in three-dimensional culture model on a novel microfabricated scaffold / Yoshitaka Miyagawa, Hajime Okita, and Nobutaka Kiyokawa -- Analyzing PPARa/ligand interactions by chemical cross-linking and high-resolution mass spectrometry / Mathias Q. Muller, Christian H. Ihling, and Andrea Sinz -- Synthesis, mass spectrometric characterization, and analysis of the PPARd agonist GW1516 and its major human metabolites : targets in sports drug testing / Mario Thevis [and others] -- LC-MS-based method for the qualitative and quantitative analysis of the novel PPARg agonist KR-62980 / Min-Sun Kim and Myung Ae Bae -- Behavioral paradigms to evaluate PPAR modulation in animal models of brain injury / Dana Greene-Schloesser, Caroline I. Schnegg, and Mike E. Robbins.

Includes bibliographical references and index.

Print version record.

All three peroxisome proliferator-activated receptor (PPAR) subtypes share a high degree of structural homology but differ in function, tissue distribution and ligand specificity. PPARs play critical roles as regulators of numerous physiological as well as pathophysiological pathways, and efforts are currently underway to fully characterize their functioning and to develop safer and more effective PPAR modulators to treat a myriad of diseases and conditions. In Peroxisome proliferator-Activated Receptors: Methods and Protocols, renowned experts in the PPAR arena provide detailed protocols for investigating these receptors. Chapters contain methods ranging from the cloning of receptors to their knockdown, to protocols exploring posttranslational modifications of PPARs and coactivators, as well as receptor subcellular localization. Also assembled are methods to evaluate the involvement of these receptors in behavior functions, an emerging facet in PPAR research. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. With its well-honed methodologies, Peroxisome proliferator-Activated Receptors: Methods and Protocols, will be a useful resource for all seeking to advance their knowledge of this field.

English.