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Clinical proteomics : methods and protocols / edited by Antonia Vlahou, Manousos Makridakis.

Contributor(s): Material type: TextTextSeries: Methods in molecular biology (Clifton, N.J.) ; volume 1243.Publisher: Totowa, NJ : Humana Press, [2015]Copyright date: ©2015Edition: Second editionDescription: 1 online resource (xiii, 278 pages) : illustrationsContent type:
  • text
Media type:
  • computer
Carrier type:
  • online resource
ISBN:
  • 9781493918720
  • 1493918729
  • 1493918710
  • 9781493918713
Subject(s): Genre/Form: Additional physical formats: Print version:: Clinical proteomics.DDC classification:
  • 616.07/56 22
LOC classification:
  • QP519.9.M3
NLM classification:
  • W1
  • QU 25
Online resources:
Contents:
Biological sample collection for clinical proteomics : existing SOPs / Vasiliki Lygirou, Manousos Makridakis, and Antonia Vlahou -- Targeting the proteome of cellular fractions : focus on secreted proteins / Agnieszka Latosinska ... [and 4 others] -- Preparation of urinary exosomes : methodological issues for clinical proteomics / Marina Pitto, Samuele Corbetta, and Francesca Raimondo -- Sample treatment methods involving combinatorial peptide ligand libraries for improved proteomes analyses / Pier Giorgio Righetti and Egisto Boschetti -- Glycoprotein enrichment method Using a selective magnetic nano-probe platform (MNP) functionalized with lectins / Marta Cova ... [and 6 others] -- Latest advancements in proteomic two-dimensional gel electrophoresis analysis applied to biological samples / Laura Santucci ... [and 3 others] -- 2DE maps in the discovery of human autoimmune kidney diseases : the case of membranous glomerulonephritis / Maurizio Bruschi, Laura Santucci, Gian Marco Ghiggeri, and Giovanni Candiano-- MALDI-imaging mass spectrometry on tissues / Veronica Mainini ... [and 5 others] -- Laser capture microdissection of fluorescently labeled amyloid plaques from alzheimer's disease brain tissue for mass spectrometric analysis / Diana A.T. Nijholt, Christoph Stingl, and Theo M. Luider -- Urine sample preparation and fractionation for global proteome profiling by LC-MS / Magali Court, Jérôme Garin, and Christophe D. Masselon -- Methods in capillary electrophoresis coupled to mass spectrometry for the identification of clinical proteomic/peptidomic biomarkers in biofluids / Angelique Stalmach ... [and 5 others] -- Quantification of proteins in urine samples using targeted mass spectrometry methods / Nina Khristenko and Bruno Domon -- Statistical issues in the design and planning of proteomic profiling experiments / David A. Cairns -- Integrating proteomics profiling data sets : a network perspective / Akshay Bhat, Mohammed Dakna, and Harald Mischak -- European medicines agency experience with biomarker qualification / Efthymios Manolis ... [and 3 others].
Summary: This second edition expands upon the previous edition with current, detailed developments in the field and brings together a multi-disciplinary team of leading researchers to provide their latest protocols for clinical proteomics analysis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and Practical, Clinical Proteomics: Methods and Protocols, Second Edition presents approaches that will serve as a reliable guide to researchers, including clinicians, chemists, molecular biologists, bioinformaticians and computational, biologists, and investigators working on biomarker development.
Holdings
Item type Current library Collection Call number Status Date due Barcode Item holds
eBook eBook e-Library eBook Protocols Available
Total holds: 0

Includes bibliographical references and index.

Biological sample collection for clinical proteomics : existing SOPs / Vasiliki Lygirou, Manousos Makridakis, and Antonia Vlahou -- Targeting the proteome of cellular fractions : focus on secreted proteins / Agnieszka Latosinska ... [and 4 others] -- Preparation of urinary exosomes : methodological issues for clinical proteomics / Marina Pitto, Samuele Corbetta, and Francesca Raimondo -- Sample treatment methods involving combinatorial peptide ligand libraries for improved proteomes analyses / Pier Giorgio Righetti and Egisto Boschetti -- Glycoprotein enrichment method Using a selective magnetic nano-probe platform (MNP) functionalized with lectins / Marta Cova ... [and 6 others] -- Latest advancements in proteomic two-dimensional gel electrophoresis analysis applied to biological samples / Laura Santucci ... [and 3 others] -- 2DE maps in the discovery of human autoimmune kidney diseases : the case of membranous glomerulonephritis / Maurizio Bruschi, Laura Santucci, Gian Marco Ghiggeri, and Giovanni Candiano-- MALDI-imaging mass spectrometry on tissues / Veronica Mainini ... [and 5 others] -- Laser capture microdissection of fluorescently labeled amyloid plaques from alzheimer's disease brain tissue for mass spectrometric analysis / Diana A.T. Nijholt, Christoph Stingl, and Theo M. Luider -- Urine sample preparation and fractionation for global proteome profiling by LC-MS / Magali Court, Jérôme Garin, and Christophe D. Masselon -- Methods in capillary electrophoresis coupled to mass spectrometry for the identification of clinical proteomic/peptidomic biomarkers in biofluids / Angelique Stalmach ... [and 5 others] -- Quantification of proteins in urine samples using targeted mass spectrometry methods / Nina Khristenko and Bruno Domon -- Statistical issues in the design and planning of proteomic profiling experiments / David A. Cairns -- Integrating proteomics profiling data sets : a network perspective / Akshay Bhat, Mohammed Dakna, and Harald Mischak -- European medicines agency experience with biomarker qualification / Efthymios Manolis ... [and 3 others].

This second edition expands upon the previous edition with current, detailed developments in the field and brings together a multi-disciplinary team of leading researchers to provide their latest protocols for clinical proteomics analysis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and Practical, Clinical Proteomics: Methods and Protocols, Second Edition presents approaches that will serve as a reliable guide to researchers, including clinicians, chemists, molecular biologists, bioinformaticians and computational, biologists, and investigators working on biomarker development.

Online resource; title from PDF title page (SpringerLink, viewed November 17, 2014).

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