RNA nanotechnology and therapeutics : methods and protocols / edited by Peixuan Guo, Farzin Haque.

Includes bibliographical references and index.

Overview of methods in RNA nanotechnology : synthesis, purification, and characterization of RNA nanoparticles / Farzin Haque and Peixuan Guo -- Multiple approaches for the investigation of bacterial small regulatory RNAs self-assembly / Christophe Lavelle, Florent Busi, and Véronique Arluison -- Measuring the elasticity of ribonucleotide(s)-containing DNA molecules using AFM / Kyung Duk Koh [and others] -- Silver nanoclusters for RNA nanotechnology : steps towards visualization and tracking of RNA nanoparticle assemblies / Kirill A. Afonin [and others] -- Large scale purification of RNA nanoparticles by preparative ultracentrifugation / Daniel L. Jasinski [and others] -- HPLC purification of RNA aptamers up to 59 nucleotides with single-nucleotide resolution / Zhen Huang [and others] -- Using RNA nanoparticles with thermostable motifs and fluorogenic modules for real-time detection of RNA folding and turnover in prokaryotic and eukaryotic cells / Hui Zhang [and others] -- Fluorescence labeling of short RNA by oxidation at the 3'-end / Chen Qiu, Wang-Yi Liu, and Yong-Zhen Xu -- Methods and assays for specific targeting and delivery of RNA nanoparticles to cancer metastases / Piotr Rychahou [and others] -- Functional assays for specific targeting and delivery of RNA nanoparticles to brain tumor / Tae Jin Lee [and others] -- Aptamer-mediated nanoparticle interactions : from oligonucleotide-protein complexes to SELEX screens / Laetitia Evadé [and others] -- Methods for assembling B-cell lymphoma specific and internalizing aptamer-siRNA nanoparticles via the sticky bridge / Jiehua Zhou, John J. Rossi, and Ka To Shum -- High-throughput screening assay for the functional delivery of splice-switching oligonucleotides in human melanoma cells / John M. Deanand Robert K. DeLong -- Design, assembly, and evaluation of RNA-protein nanostructures / Hirohisa Ohno, Eriko Osada, and Hirohide Saito -- Mapping RNA interactions to proteins in virions using CLIP-Seq / Baochang Fan, Peng Ni, and C. Cheng Kao -- Mapping protein-RNA interactions by RCAP, RNA-cross-linking and peptide fingerprinting / Robert C. Vaughan and C. Cheng Kao.

Online resource; title from PDF title page (SpringerLink, viewed April 29, 2015).

This volume contains a compilation of techniques and laboratory protocols directly related to RNA nanotechnology and its applications in nanobiotechnology and nanomedicine. The chapters in this book cover a wide range of research methods that can be easily comprehended and carried out in a step-by-step manner by graduate students and postdoctoral fellows from diverse scientific disciplines. Some of the covered topics include various biochemical, biophysical and bioinformatics approaches for analyzing RNA structures and properties; methods to analyze the multi-step assembly process of RNA nanoarchitectures; purification of multifunctional RNA nanoparticles by ultracentrifugation and HPLC; real-time detection of RNA nanoconstructs in vivo; conjugation of imaging, targeting and therapeutic modules to RNA scaffold; and design and characterization of RNA-protein nanostructures. Written in the highly successful Methods of Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubles troubleshooting and avoiding known pitfalls. Cutting-edge and practical, RNA Nanotechnology and Therapeutics: Methods and Protocols propels the field toward new heights by engaging researchers working in the diverse fields of physical, engineering, and life sciences.