Cartilage tissue engineering : methods and protocols / edited by Pauline M. Doran.

Includes bibliographical references and index.

Cartilage tissue engineering : what have we learned in practice / Pauline M. Doran -- Human fetal and adult chondrocytes / Kifah Shahin, Nastaran Mahmoudifar, and Pauline M. Doran -- Mesenchymal stem cells derived from human bone marrow / Oliver F.W. Gardner, Mauro Alini, and Martin J. Stoddart -- Mesenchymal stem cells derived from human adipose tissue / Nastaran Mahmoudifar and Pauline M. Doran -- Derivation and chondrogenic commitment of human embryonic stem cell-derived mesenchymal progenitors / Hicham Drissi [and others] -- Differentiation of human induced pluripotent stem cells to chondrocytes / Rosa M. Guzzo and Hicham Drissi -- Gene transfer and gene silencing in stem cells to promote chondrogenesis / Feng Zhang and Dong-An Wang -- Hydrogels with tunable properties / Peggy P.Y. Chan -- Decellularized extracellular matrix scaffolds for cartilage regeneration / Shraddha Thakkar, Hugo Fernandes, and Lorenzo Moroni -- Use of interim scaffolding and neotissue development to produce a scaffold-free living hyaline cartilage graft / Ting Ting Lau [and others] -- Bioprinted scaffolds for cartilage tissue engineering / Hyun-Wook Kang, James J. Yoo, and Anthony Atala -- Scaffolds for controlled release of cartilage growth factors / Marie Morille [and others] -- Nanostructured capsules for cartilage tissue engineering / Clara R. Correia, Rui L. Reis, and João F. Mano -- Stratified scaffolds for osteochondral tissue engineering / Patcharakamon Nooeaid, Gundula Schulze-Tanzil, and Aldo R. Boccaccini -- Mechanobioreactors for cartilage tissue engineering / Joanna F. Weber, Roman Perez, and Stephen D. Waldman -- Shear and compression bioreactor for cartilage synthesis / Kifah Shahin and Pauline M. Doran -- Microbioreactors for cartilage tissue engineering / Yu-Han Chang and Min-Hsien Wu -- Transplantation of tissue-engineered cartilage in an animal model (xenograft and autograft) : construct validation / Hitoshi Nemoto, Deborah Watson, and Koichi Masuda -- Proteomic analysis of engineered cartilage / Xinzhu Pu and Julia Thom Oxford -- Mechanical testing of cartilage constructs / Dinorath Olvera, Andrew Daly, and Daniel John Kelly.

This volume aims to describe clearly and in detail the key practical skills involved in cartilage tissue engineering. Methods are outlined for isolation and expansion of chondrocytes and stem cells; differentiation; synthesis and application of three-dimensional scaffolds; design and operation of bioreactors; in vivo testing of engineered constructs; and molecular and functional analysis of cartilage cells and tissues.