Plant signal transduction : methods and protocols / edited by Jose R. Botella, Miguel A. Botella.

Includes bibliographical references and index.

Suppressor screens in Arabidopsis / Xin Li and Yuelin Zhang -- Quantitative reverse transcription-qPCR-based gene expression analysis in plants / Heithem Ben Abdallah and Petra Bauer -- DNA-binding factor target identification by chromatin immunoprecipitation (ChIP) in plants / David Posé and Levi Yant -- Identification of genes responsible for natural variation in volatile content using next-generation sequencing technology / Iraida Amaya, Jeremy Pillet, and Kevin M. Folta -- Micro-tom tomato as an alternative plant model system : mutant collection and efficient transformation / Masahito Shikata and Hiroshi Ezura -- Culture of the tomato micro-tom cultivar in greenhouse / Christophe Rotha [and others] -- Virus-induced gene silencing as a tool to study tomato fruit biochemistry / Elio Fantini and Giovanni Giuliano -- Simplified and rapid method for the isolation and transfection of Arabidopsis leaf mesophyll protoplasts for large-scale applications / Arnaldo L. Schapire and L. Maria Lois -- Preparation of epidermal peels and guard cell protoplasts for cellular, electrophysiological, and -omics assays of guard cell function / Mengmeng Zhu [and others] -- Application of variable angle total internal reflection fluorescence microscopy to investigate protein dynamics in intact plant cells / Yinglang Wan [and others] -- Immunoprecipitation of plasma membrane receptor-like kinases for identification of phosphorylation sites and associated proteins / asuhiro Kadota, Alberto P. Macho, and Cyril Zipfel -- Yeast three-hybrid system for the detection of protein-protein interactions / Natsumi Maruta, Yuri Trusov, and Jose R. Botella -- Cautions in measuring in vivo interactions using FRET and BiFC in Nicotiana benthamiana / Meral Tunc-Ozdemir, Yan Fu, and Alan M. Jones -- Analysis of protein-lipid interactions using purified C2 domains / Jessica Pérez-Sancho [and others] -- Assessing kinase activity in plants with in-gel kinase assays / Pengcheng Wang and Jian-Kang Zhu -- Analyses of plant UDP-dependent glycosyltransferases to identify their volatile substrates using recombinant proteins / Yusuke Kamiyoshihara, Denise M. Tieman, and Harry J. Klee -- Competitive index : mixed infection-based virulence assays for genetic analysis in Pseudomonas syringae-plant interactions / Alberto P. Macho ... [e tal.] -- Simplified assays for evaluation of resistance to Alternaria brassicicola and turnip mosaic virus / Yuri Trusov [and others] -- Simultaneous determination of plant hormones by GC-TOF-MS / José G. Vallarino and Sonia Osorio.