Long non-coding RNAs : methods and protocols / edited by Yi Feng, Lin Zhang.

Includes bibliographical references and index.

LncRNA pulldown combined with mass spectrometry to identify the novel LncRNA-associated proteins / Zhen Xing, Chunru Lin, and Liuqing Yang -- Cross-linking immunoprecipitation and qPCR (CLIP-qPCR) analysis to map interactions between long noncoding RNAs and RNA-binding proteins / Je-Hyun Yoon and Myriam Gorospe -- Characterization of long noncoding RNA-associated proteins by RNA-immunoprecipitation / Youyou Zhang [and others] -- Isolation of protein complexes associated with long noncoding RNAs / Kiranmai Gumireddy, Jinchun Yan, and Qihong Huang -- Profiling long noncoding RNA expression using custom-designed microarray / Xinna Zhang [and others] -- Long noncoding RNA expression profiling using arraystar LncRNA microarrays / Yanggu Shi and Jindong Shang -- Nuclear RNA isolation and sequencing / Navroop K. Dhaliwal and Jennifer A. Mitchell -- Targeted LncRNA sequencing with the SeqCap RNA enrichment system / John C. Tan [and others] -- ChIP-Seq : library preparation and sequencing / Karyn L. Sheaffer and Jonathan Schug -- Stellaris® RNA fluorescence in situ hybridization for the simultaneous detection of immature and mature long noncoding RNAs in adherent cells / Arturo V. Orjalo Jr. and Hans E. Johansson -- Simultaneous RNA-DNA FISH / Lan-Tian Lai [and others] -- Visualizing long noncoding RNAs on chromatin / Michael Hinten [and others] -- Non-isotopic method for in situ LncRNA visualization and quantitation / Botoul Maqsodi and Corina Nikoloff -- Detection of long noncoding RNA expression by nonradioactive northern blots / Xiaowen Hu [and others] -- Assessment of in vivo SiRNA delivery in cancer mouse models / Hiroto Hatakeyama [and others] -- Targeting long noncoding RNA with antisense oligonucleotide technology as cancer therapeutics / Tianyuan Zhou, Youngsoo Kim, and A. Robert MacLeod -- Characterization of circular RNAs / Yang Zhang, Li Yang, and Ling-Ling Chen -- Methods for characterization of alternative RNA splicing / Samuel E. Harvey and Chonghui Cheng -- NONCODEv4 : annotation of noncoding RNAs with emphasis on long noncoding RNAs / Yi Zhao, Jiao Yuan, and Runsheng Chen -- Computational analysis of LncRNA from cDNA sequences / Susan Boerner and Karen M. McGinnis -- Analyzing MiRNA-LncRNA interactions / Maria D. Paraskevopoulou and Artemis G. Hatzigeorgiou -- Long noncoding RNAs : an overview / Dongmei Zhang [and others].

Online resource; title form PDF title page (SpringerLink, viewed January 4, 2016).

This volume assembles a broad spectrum of methods used in long non-coding RNAs (lncRNA) research, ranging from computational annotation of lncRNA genes to molecular and cellular analyses of the function of individual lncRNA. Long Non-Coding RNAs: Methods and Protocols also discusses methods used to study circular RNAs and RNA splicing, as well as influential findings on lncRNA in human diseases. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Thorough and cutting-edge, Long Non-Coding RNAs: Methods and Protocols is a must-have for molecular biologists, cell and developmental biologists, specialists who conduct disease-oriented research, and bioinformatics experts who seek a better understanding on lncRNA expression and function by computational analysis of the massive sequencing data that are rapidly accumulating in recent years.