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Membrane proteins : Production and functional characterization / edited by Arun K. Shukla.

Contributor(s): Material type: TextTextSeries: Methods in enzymology ; v. 556.Publisher: Amsterdam, Netherlands ; Boston, Mass. : Elsevier/ Academic Press, 2015Copyright date: ©2015Description: 1 online resource (xxiv, 651 pages and 14 pages of plates) : illustrations (some color)Content type:
  • text
Media type:
  • computer
Carrier type:
  • online resource
ISBN:
  • 0128016256
  • 9780128016251
Other title:
  • Production and functional characterization
Subject(s): Genre/Form: Additional physical formats: Print version:: Membrane proteins.DDC classification:
  • 572/.696 23
LOC classification:
  • QP552.M44 M45 2015
NLM classification:
  • QU 55.7
Online resources:
Contents:
Engineering Escherichia coli for functional expression of membrane proteins -- ACEMBLing a multiprotein transmembrane complex: the functional SecYEG-SecDF-YajC-YidC holotranslocon protein secretase/insertase -- Expression of purification of OsVDAC4 -- Membrane protein expression in Lactococcus lactis -- An unconventional anaerobic membrane protein production system based on Wolinella succinogenes -- Membrane protein expression and analysis in yeast -- Heterologous expression of G-protein-coupled receptors in yeast -- Recombinant G protein-coupled receptor expression in Saccharomyces cerevisiae for protein characterization -- Baculovirus-mediated expression of GPCRs in insect cells -- Expression of membrane proteins in the eyes of transgenic Drosophila melangaster -- Rapid method to express and purify human membrane protein using the Xenopus Oocyte system for functional and low-resolution structural anlaysis -- Expression of G protein-coupled receptors in mammalian cells -- Construction of stable mammalian cell lines for inducible expression of G protein-coupled receptors -- Rapid and facile recombinant expression of bovine rhodopsin in HEK293S GnTI- cells using a PiggyBac inducible system -- Semliki forest virus-based expression of recombinant GPCRs.
Screening for lipid requirements of membrane proteins by combining cell-free expression with nanodiscs -- Liposome reconstitution and transport assay for recombinant transporters -- Lipid reconstitution and recording of recombinant ion channels -- Reconstitution of membrane proteins: a GPCR as an example -- Ion channel reporter for monitoring the activity of engineered GPCRs -- Methods for labeling skeletal muscle ion channels site-specifically with fluorophores suitable for FRET-based structural analysis -- Structural and fucntional studies of NirC from Salmonella typhimurium -- Surface plasmon resonance analysis of seven-transmembrane receptors -- Cross-linking strategies to study peptide ligand-receptor ineractions -- From recombinant expression to crystals: a step-by-step guide to GPCR crystallography -- Structure-based biophysical analysis of the interaction of rhodopsin with G protein and arrestin.
Summary: Membrane Proteins - Production and Function Characterization a volume of Methods in Enzymology, encompasses chapters from the leading experts in the area of membrane protein biology. The chapters provide a brief overview of the topics covered and also outline step-by-step protocol. Illustrations and case example images are included wherever appropriate to help the readers understand the schematics and general experimental outlines.
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Engineering Escherichia coli for functional expression of membrane proteins -- ACEMBLing a multiprotein transmembrane complex: the functional SecYEG-SecDF-YajC-YidC holotranslocon protein secretase/insertase -- Expression of purification of OsVDAC4 -- Membrane protein expression in Lactococcus lactis -- An unconventional anaerobic membrane protein production system based on Wolinella succinogenes -- Membrane protein expression and analysis in yeast -- Heterologous expression of G-protein-coupled receptors in yeast -- Recombinant G protein-coupled receptor expression in Saccharomyces cerevisiae for protein characterization -- Baculovirus-mediated expression of GPCRs in insect cells -- Expression of membrane proteins in the eyes of transgenic Drosophila melangaster -- Rapid method to express and purify human membrane protein using the Xenopus Oocyte system for functional and low-resolution structural anlaysis -- Expression of G protein-coupled receptors in mammalian cells -- Construction of stable mammalian cell lines for inducible expression of G protein-coupled receptors -- Rapid and facile recombinant expression of bovine rhodopsin in HEK293S GnTI- cells using a PiggyBac inducible system -- Semliki forest virus-based expression of recombinant GPCRs.

Screening for lipid requirements of membrane proteins by combining cell-free expression with nanodiscs -- Liposome reconstitution and transport assay for recombinant transporters -- Lipid reconstitution and recording of recombinant ion channels -- Reconstitution of membrane proteins: a GPCR as an example -- Ion channel reporter for monitoring the activity of engineered GPCRs -- Methods for labeling skeletal muscle ion channels site-specifically with fluorophores suitable for FRET-based structural analysis -- Structural and fucntional studies of NirC from Salmonella typhimurium -- Surface plasmon resonance analysis of seven-transmembrane receptors -- Cross-linking strategies to study peptide ligand-receptor ineractions -- From recombinant expression to crystals: a step-by-step guide to GPCR crystallography -- Structure-based biophysical analysis of the interaction of rhodopsin with G protein and arrestin.

Membrane Proteins - Production and Function Characterization a volume of Methods in Enzymology, encompasses chapters from the leading experts in the area of membrane protein biology. The chapters provide a brief overview of the topics covered and also outline step-by-step protocol. Illustrations and case example images are included wherever appropriate to help the readers understand the schematics and general experimental outlines.

Includes bibliographical references and indexes.

English.

Description based on online resource; title from digital title page (viewed on February 26, 2021).

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