Isolation of plant organelles and structures : methods and protocols / edited by Nicolas L. Taylor, A. Harvey Millar.

Includes bibliographical references and index.

Isolation of plant organelles and structures in the post-genomic era / A. Harvey Millar and Nicolas L. Taylor -- Approaches to characterize organelle, compartment, or structure purity / Stefanie J. Mueller, Sebastian N.W. Hoernstein, and Ralf Reski -- Isolation of nuclei and nucleoli / Alison F. Pendle and Peter J. Shaw -- Isolation and suborganellar fractionation of Arabidopsis chloroplasts / Úrsula Flores-Pérez and Paul Jarvis -- Isolation of chromoplasts and suborganellar compartments from tomato and bell pepper fruit / Cristina Barsan, Marcel Kuntz, and Jean-Claude Pech -- Leucoplast isolation and subfractionation / William C. Plaxton -- Isolation of mitochondria, their sub-organellar compartments, and membranes / Owen Duncan, A. Harvey Millar, and Nicolas L. Taylor -- Isolation of Arabidopsis leaf peroxisomes and the peroxisomal membrane / Sigrun Reumann and Piotr Lisik -- Isolation of vacuoles and the tonoplast / Jan Zouhar -- Isolation of endoplasmic reticulum and its membrane / G. Eric Schaller -- Enrichment of golgi membranes from Triticum aestivum (wheat) seedlings / Wei Zeng [and others] -- Isolation of autolysosomes from tobacco BY-2 cells / Chihiro Takatsuka, Yuko Inoue-Aono, and Yuji Moriyasu -- Isolation of protein storage vacuoles and their membranes / Tomoo Shimada and Ikuko Hara-Nishimura -- Isolation of the cell wall / Hervé Canut, Cécile Albenne, and Elisabeth Jamet -- Isolation of plasmodesmata / Christine Faulkner and Emmanuelle M.F. Bayer -- Isolation of plasma membrane and plasma membrane microdomains / Anzu Minami [and others] -- Enrichment of the plant cytosolic fraction / Jeemeng Lao [and others] -- Isolation of apoplast / Erik Andreasson, Kibrom B. Abreha, and Svante Resjö -- Isolation of cytosolic ribosomes / Hanna Klang Årstrand and Maria V. Turkina -- Isolation of plastid ribosomes / Kenichi Yamaguchi -- Isolation of mitochondrial ribosomes / Adam J. Carroll -- Isolation of microtubules and microtubule-associated proteins / Takahiro Hamada and Seiji Sonobe -- Isolation of actin and actin-binding proteins / Etsuo Yokota -- Purification of 26S proteasomes and their subcomplexes from plants / Richard S. Marshall, David C. Gemperline, and Richard D. Vierstra.

Online resource; title from PDF title page (SpringerProtocol, viewed October 14, 2016).

This book brings together the major techniques used in the isolation or enrichment of individual populations of organelles and other subcellular structures from plants with the goal that, by being able to isolate subcellular structures, the research and understanding of various facets of compartmentalized function in plant cells can be advanced. Written for the highly successful Methods in Molecular Biology series, expert contributors provide chapters that contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Isolation of Plant Organelles and Structures: Methods and Protocols will greatly aid those who regularly isolate subcellular components as well as those whose research has lead them to focus on a subcellular compartment or a particular process for the first time, thus producing the need to be able to isolate it or enrich it for study.