Mycotoxigenic fungi : methods and protocols / edited by Antonio Moretti, Antonia Susca.

Includes bibliographical references and index.

Mycotoxins : an underhand food problem / Antonio Moretti, Antonio F. Logrieco, and Antonia Susca -- Alternaria species and their associated mycotoxins / Virginia Elena Fernández Pinto and Andrea Patriarca -- Aspergillus species and their associated mycotoxins / Giancarlo Perrone and Antonia Gallo -- Fusarium species and their associated mycotoxins / Gary P. Munkvold -- Penicillium species and their associated mycotoxins / Giancarlo Perrone and Antonia Susca -- Targeting conserved genes in Alternaria species / Miguel Ángel Pavón, Inés María López-Calleja, Isabel González, Rosario Martín, and Teresa García -- Targeting conserved genes in Aspergillus species / Sándor Kocsubé and János Varga -- Targeting conserved genes in Fusarium species / Jéssica Gil-Serna, Belén Patiño, Miguel Jurado, Salvador Mirete, Covadonga Vázquez, and M. Teresa González-Jaén -- Targeting conserved genes in Penicillium species / Stephen W. Peterson -- Targeting aflatoxin biosynthetic genes / Ali Y. Srour, Ahmad M. Fakhoury, and Robert L. Brown -- Targeting trichothecene biosynthetic genes / Songhong Wei, Theo van der Lee, Els Verstappen, Marga van Gent, and Cees Waalwijk -- Targeting ochratoxin biosynthetic genes / Antonia Gallo and Giancarlo Perrone -- Targeting fumonisin biosynthetic genes / Robert H. Proctor and Martha M. Vaughan -- Targeting other mycotoxin biosynthetic genes / María J. Andrade, Mar Rodríguez, Juan J. Córdoba, and Alicia Rodríguez -- Evaluating aflatoxin gene expression in Aspergillus section flavi / Paula Cristina Azevedo Rodrigues, Jéssica Gil-Serna, and M. Teresa González-Jaén -- Evaluating fumonisin gene expression in Fusarium verticillioides / Valeria Scala, Ivan Visentin, and Francesca Cardinale -- Multiplex detection of Aspergillus species / Pedro Martínez-Culebras, María Victoria Selma, and Rosa Aznar -- Multiplex detection of Fusarium species / Tapani Yli-Mattila, Siddaiah Chandra Nayaka, Mudili Venkataramana, and Emre Yörük -- Multiplex detection of toxigenic Penicillium species / Alicia Rodríguez, Juan J. Córdoba, Mar Rodríguez, and María J. Andrade -- PCR-RFLP for Aspergillus species / Ali Atoui and André El Khoury -- PCR ITS-RFLP for Penicillium species and other genera / Sandrine Rousseaux and Michèle Guilloux-Bénatier -- Identification of ochratoxin A-producing black Aspergilli from grapes using loop-mediated isothermal amplification (LAMP) assays / Michelangelo Storari and Giovanni A.L. Broggini -- Detection of transcriptionally active mycotoxin gene clusters : DNA microarray / Tamás Emri, Anna Zalka, and István Pócsi -- Mycotoxins : a fungal genomics perspective / Daren W. Brown and Scott E. Baker.

Online resource; title from PDF title page (SpringerProtocol, viewed December 9, 2016).

This thorough volume explores the possibility of detecting and identifying toxigenic fungi, able to produce secondary metabolites known as mycotoxins, which cause severe health problems in humans and animals after exposure to contaminated food and feed, having a broad range of toxic effects, including carcinogenicity, neurotoxicity, and reproductive and developmental toxicity. Beginning with a section on fungal genera and species of major significance along with their associated mycotoxins, the book continues with sections on Polymerase Chain Reaction (PCR)-based methods for the detection and identification of mycotoxigenic fungi, PCR-based methods for multiplex detection of mycotoxigenic fungi, as well as sections on combined approaches and new methodologies. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Mycotoxigenic Fungi: Methods and Protocols will aid researchers working in this vital field to provide insight into possible actions to reduce mycotoxin contamination of crop plants and the food/feed byproducts.