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Functional genomics in Medicago truncatula : methods and protocols / edited by Luis A. Cañas and José Pío Beltrán.

Contributor(s): Material type: TextTextSeries: Methods in molecular biology (Clifton, N.J.) ; v. 1822.Publisher: New York : Humana Press : Springer, [2018]Copyright date: ©2018Description: 1 online resource (xi, 343 pages) : illustrations (some color)Content type:
  • text
Media type:
  • computer
Carrier type:
  • online resource
ISBN:
  • 9781493986330
  • 1493986333
Subject(s): Genre/Form: Additional physical formats: Printed edition:: No titleDDC classification:
  • 633.3 23
LOC classification:
  • SB205.M4
Online resources:
Contents:
Grain and forage legumes : nutritional value and agriculture sustainability / José Pío Beltrán and Luis A. Cañas -- Model legumes : functional genomics tools in Medicago truncatula / Luis A. Cañas and José Pío Beltrán -- Medicago truncatula genome : genomic data availability / David Burks, Rajeev Azad, Jiangqi Wen, and Rebecca Dickstein -- Physical mutagenesis in Medicago truncatula using fast neutron bombardment (FNB) for symbiosis and developmental biology studies / Yuhui Chen and Rujin Chen -- Targeting induced local lesions IN genomes (TILLING) in Medicago truncatula / Myriam Sánchez, Christine Le Signor, Gregoire Aubert, Brigitte Darchy, Karine Gallardo, and Richard D. Thompson -- T-DNA insertional mutagenesis and activation tagging in Medicago truncatula / Francesco Panara, Ornella Calderini, and Andrea Porceddu -- Tnt1 insertional mutagenesis in Medicago truncatula / Hee-Kyung Lee, Kirankumar S. Mysore, and Jiangqi Wen -- Transient posttranscriptional gene silencing in Medicago truncatula : virus-induced gene silencing (VIGS) / Joanna Serwatowska, Ole Søgaard Lund, and Ida Elisabeth Johansen -- Stable inactivation of microRNAs in Medicago truncatula roots / Hélène Proust, Jérémie Bazin, Céline Sorin, Caroline Hartmann, Martin Crespi, and Christine Lelandais-Brière -- Non-isotopic RNA in situ hybridization for functional analyses in Medicago truncatula / Concepción Gómez-Mena and Edelín M. Roque -- In situ hybridization method for localization of mRNA molecules in medicago tissue sections / Olga Kulikova, Carolien Franken, and Ton Bisseling -- Editing the Medicago truncatula genome : targeted mutagenesis using the CRISPR-Cas9 reagent / Shaun J. Curtin -- Functional genomics and seed development in Medicago truncatula : an overview / Christine Le Signor, Vanessa Vernoud, Mélanie Noguero, Karine Gallardo, and Richard D. Thompson -- Functional genomics and genetic control of compound leaf development in Medicago truncatula : an overview / Rujin Chen -- Root development in Medicago truncatula : lessons from genetics to functional genomics / Hélène Proust, Caroline Hartmann, Martin Crespi, and Christine Lelandais-Brière -- Multiple faces of the medicago-sinorhizobium symbiosis / Fathi Berrabah, El Hosseyn Ait Salem, Marie Garmier, and Pascal Ratet -- Functional genomics and flowering time in Medicago truncatula : an overview / James L. Weller and Richard C. Macknight -- Functional genomics and genetic control of flower and fruit development in Medicago truncatula : an overview / Edelín Roque, Concepción Gómez-Mena, Cristina Ferrándiz, José Pío Beltrán, and Luis A. Cañas -- Toward unravelling the genetic determinism of the acquisition of salt and osmotic stress tolerance through in vitro selection in Medicago truncatula / Adel M. Elmaghrabi, Hilary J. Rogers, Dennis Francis, and Sergio Ochatt -- Functional genomics in the study of metabolic pathways in Medicago truncatula : an overview / Chenggang Liu, Chan Man Ha, and Richard A. Dixon.
Summary: This volume discusses popular methods to achieve different types of mutagenesis and forward/reverse genetics in Medicago truncatula. Several studies on genetic control of developmental and metabolic processes in this model legume are also described. The chapters in this book cover topics such as Targeting Induced Local Lesions IN Genomes (TILLING), Fast Neutron Bombardment (FNB), Tnt1 insertional mutagenesis, Virus-Induced Gene Silencing (VIGS), stable inactivation of microRNAs in roots, gene editing by CRISPR-Cas9, etc. This book also contains reviews on the specific use of these techniques in functional studies on the genetic control of seed, leaf, root, nodule, floral and fruit development in M. truncatula. Written for the highly successful Methods in Molecular Biology series format, chapters contain the kind of detailed description and implementation advice needed to promote success in the lab. Cutting-edge and thorough, Functional Genomics in Medicago truncatula: Methods and Protocols is a valuable resource for anyone interested in learning more about this developing field.
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Includes bibliographical references and index.

Grain and forage legumes : nutritional value and agriculture sustainability / José Pío Beltrán and Luis A. Cañas -- Model legumes : functional genomics tools in Medicago truncatula / Luis A. Cañas and José Pío Beltrán -- Medicago truncatula genome : genomic data availability / David Burks, Rajeev Azad, Jiangqi Wen, and Rebecca Dickstein -- Physical mutagenesis in Medicago truncatula using fast neutron bombardment (FNB) for symbiosis and developmental biology studies / Yuhui Chen and Rujin Chen -- Targeting induced local lesions IN genomes (TILLING) in Medicago truncatula / Myriam Sánchez, Christine Le Signor, Gregoire Aubert, Brigitte Darchy, Karine Gallardo, and Richard D. Thompson -- T-DNA insertional mutagenesis and activation tagging in Medicago truncatula / Francesco Panara, Ornella Calderini, and Andrea Porceddu -- Tnt1 insertional mutagenesis in Medicago truncatula / Hee-Kyung Lee, Kirankumar S. Mysore, and Jiangqi Wen -- Transient posttranscriptional gene silencing in Medicago truncatula : virus-induced gene silencing (VIGS) / Joanna Serwatowska, Ole Søgaard Lund, and Ida Elisabeth Johansen -- Stable inactivation of microRNAs in Medicago truncatula roots / Hélène Proust, Jérémie Bazin, Céline Sorin, Caroline Hartmann, Martin Crespi, and Christine Lelandais-Brière -- Non-isotopic RNA in situ hybridization for functional analyses in Medicago truncatula / Concepción Gómez-Mena and Edelín M. Roque -- In situ hybridization method for localization of mRNA molecules in medicago tissue sections / Olga Kulikova, Carolien Franken, and Ton Bisseling -- Editing the Medicago truncatula genome : targeted mutagenesis using the CRISPR-Cas9 reagent / Shaun J. Curtin -- Functional genomics and seed development in Medicago truncatula : an overview / Christine Le Signor, Vanessa Vernoud, Mélanie Noguero, Karine Gallardo, and Richard D. Thompson -- Functional genomics and genetic control of compound leaf development in Medicago truncatula : an overview / Rujin Chen -- Root development in Medicago truncatula : lessons from genetics to functional genomics / Hélène Proust, Caroline Hartmann, Martin Crespi, and Christine Lelandais-Brière -- Multiple faces of the medicago-sinorhizobium symbiosis / Fathi Berrabah, El Hosseyn Ait Salem, Marie Garmier, and Pascal Ratet -- Functional genomics and flowering time in Medicago truncatula : an overview / James L. Weller and Richard C. Macknight -- Functional genomics and genetic control of flower and fruit development in Medicago truncatula : an overview / Edelín Roque, Concepción Gómez-Mena, Cristina Ferrándiz, José Pío Beltrán, and Luis A. Cañas -- Toward unravelling the genetic determinism of the acquisition of salt and osmotic stress tolerance through in vitro selection in Medicago truncatula / Adel M. Elmaghrabi, Hilary J. Rogers, Dennis Francis, and Sergio Ochatt -- Functional genomics in the study of metabolic pathways in Medicago truncatula : an overview / Chenggang Liu, Chan Man Ha, and Richard A. Dixon.

Online resource; title from PDF title page (SpringerLink, viewed July 27, 2018).

This volume discusses popular methods to achieve different types of mutagenesis and forward/reverse genetics in Medicago truncatula. Several studies on genetic control of developmental and metabolic processes in this model legume are also described. The chapters in this book cover topics such as Targeting Induced Local Lesions IN Genomes (TILLING), Fast Neutron Bombardment (FNB), Tnt1 insertional mutagenesis, Virus-Induced Gene Silencing (VIGS), stable inactivation of microRNAs in roots, gene editing by CRISPR-Cas9, etc. This book also contains reviews on the specific use of these techniques in functional studies on the genetic control of seed, leaf, root, nodule, floral and fruit development in M. truncatula. Written for the highly successful Methods in Molecular Biology series format, chapters contain the kind of detailed description and implementation advice needed to promote success in the lab. Cutting-edge and thorough, Functional Genomics in Medicago truncatula: Methods and Protocols is a valuable resource for anyone interested in learning more about this developing field.

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