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Microfluidic electrophoresis : methods and protocols / edited by Debashis Dutta.

Contributor(s): Material type: TextTextSeries: Methods in molecular biology (Clifton, N.J.) ; v. 1906.Publisher: New York : Humana Press : Springer, [2018]Copyright date: ©2019Description: 1 online resource (x, 255 pages) : illustrations (some color)Content type:
  • text
Media type:
  • computer
Carrier type:
  • online resource
ISBN:
  • 9781493989645
  • 1493989642
Subject(s): Genre/Form: Additional physical formats: Print version:: Microfluidic electrophoresis.DDC classification:
  • 541/.372 23
LOC classification:
  • QP519.9.E434
Online resources:
Contents:
Fabrication of glass microfluidic devices / Christopher T. Culbertson, Jay Sibbitts, Kathleen Sellens, and Shu Jia -- Soft lithography, molding, and micromachining techniques for polymer micro devices / Ashis Kumar Sen, Abhishek Raj, Utsab Banerjee, and Sk Rameez Iqbal -- Sample injection techniques / J ames M. Karlinsey -- Sample preconcentration protocols in microfluidic electrophoresis / Fumihiko Kitagawa and Koji Otsuka -- Microchip electrophoresis containing electrodes for integrated electrochemical detection / Lucas Paines Bressan, Dosil Pereira de Jesus, Dulan Bandara Gunasekara, Susan Marie Lunte, and Joseþ Alberto Fracassi da Silva -- Micellar electrokinetic chromatography / Braden C. Giordano, Ronald Siefert, and Greg E. Collins -- Microchip isotachophoresis : analysis of pharmaceuticals / Mariaþn Masaþ r and Jasna Hradski -- Microfluidic free-rlow isoelectric focusing with real-time pI determination / Stefan Nagl -- Nanochannel gradient separations / Michael A. Startsev and David W. Inglis -- Paper-based electrophoresis microchip as a powerful tool for bioanalytical applications / Cyro L.S. Chagas, Thiago M.G. Cardoso, and Wendell K.T. Coltro -- Band broadening theories in capillary electrophoresis / Sandip Ghosal -- Estimating stream broadening in free-flow electrophoretic systems based on the method-of-moments formulation / Debashis Dutta -- Microchip electrophoresis tools for the analysis of small molecules / Federico J.V. Gomez and Marıþa Fernanda Silva -- Integrated microfluidic system for rapid DNA fingerprint analysis : a miniaturized integrated DNA analysis system (MiDAS) -swab sample-in to DNA profile-out / Jianing Yang, Cedric Hurth, Alan Nordquist, Stan Smith, and Frederic Zenhausern -- Achieving stable electrospray ionization mass spectrometry detection from microfluidic chips / Iulia M. Lazar -- Microchip-based electrophoretic separations with a pressure-driven backflow / Ling Xia and Debashis Dutta
Summary: This detailed book provides a set of protocols necessary for the development of a variety of microchip-based electrophoretic assays. It compiles a range of such electrophoretic methods by leading researchers in the field, covering subjects such as microfluidic device fabrication, on-chip sample preparation, theoretical/simulation protocols for assessing these separation methods, as well as common practices followed when applying them to important real world applications. The contents of the book range from protocols for classical assays to those involving pioneering separation techniques recently developed by the scientific community for advancing our analytical capabilities. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Microfluidic Electrophoresis: Methods and Protocols serves as an convenient text for academic researchers as well as practicing engineers, biochemists, and analytical laboratory professionals.
Holdings
Item type Current library Collection Call number Status Date due Barcode Item holds
eBook eBook e-Library eBook Protocols Available
Total holds: 0

Includes bibliographical references and index.

Online resource; title from PDF title page (SpringerLink, viewed November 29, 2018).

Fabrication of glass microfluidic devices / Christopher T. Culbertson, Jay Sibbitts, Kathleen Sellens, and Shu Jia -- Soft lithography, molding, and micromachining techniques for polymer micro devices / Ashis Kumar Sen, Abhishek Raj, Utsab Banerjee, and Sk Rameez Iqbal -- Sample injection techniques / J ames M. Karlinsey -- Sample preconcentration protocols in microfluidic electrophoresis / Fumihiko Kitagawa and Koji Otsuka -- Microchip electrophoresis containing electrodes for integrated electrochemical detection / Lucas Paines Bressan, Dosil Pereira de Jesus, Dulan Bandara Gunasekara, Susan Marie Lunte, and Joseþ Alberto Fracassi da Silva -- Micellar electrokinetic chromatography / Braden C. Giordano, Ronald Siefert, and Greg E. Collins -- Microchip isotachophoresis : analysis of pharmaceuticals / Mariaþn Masaþ r and Jasna Hradski -- Microfluidic free-rlow isoelectric focusing with real-time pI determination / Stefan Nagl -- Nanochannel gradient separations / Michael A. Startsev and David W. Inglis -- Paper-based electrophoresis microchip as a powerful tool for bioanalytical applications / Cyro L.S. Chagas, Thiago M.G. Cardoso, and Wendell K.T. Coltro -- Band broadening theories in capillary electrophoresis / Sandip Ghosal -- Estimating stream broadening in free-flow electrophoretic systems based on the method-of-moments formulation / Debashis Dutta -- Microchip electrophoresis tools for the analysis of small molecules / Federico J.V. Gomez and Marıþa Fernanda Silva -- Integrated microfluidic system for rapid DNA fingerprint analysis : a miniaturized integrated DNA analysis system (MiDAS) -swab sample-in to DNA profile-out / Jianing Yang, Cedric Hurth, Alan Nordquist, Stan Smith, and Frederic Zenhausern -- Achieving stable electrospray ionization mass spectrometry detection from microfluidic chips / Iulia M. Lazar -- Microchip-based electrophoretic separations with a pressure-driven backflow / Ling Xia and Debashis Dutta

This detailed book provides a set of protocols necessary for the development of a variety of microchip-based electrophoretic assays. It compiles a range of such electrophoretic methods by leading researchers in the field, covering subjects such as microfluidic device fabrication, on-chip sample preparation, theoretical/simulation protocols for assessing these separation methods, as well as common practices followed when applying them to important real world applications. The contents of the book range from protocols for classical assays to those involving pioneering separation techniques recently developed by the scientific community for advancing our analytical capabilities. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Microfluidic Electrophoresis: Methods and Protocols serves as an convenient text for academic researchers as well as practicing engineers, biochemists, and analytical laboratory professionals.

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