The hippo pathway : methods and protocols / edited by Alexander Hergovich.

Includes bibliographical references and index.

Print version record.

Power of Drosophila genetics : the discovery of the hippo pathway / Rewatee Gokhale and Cathie M. Pfleger -- Drosophila genetics : the power of genetic mosaic approaches / Mardelle Atkins -- Drosophila genetics : analysis of tissue growth in adult tissues / Alexander D. Fulford and Paulo S. Ribeiro -- Live imaging of hippo pathway components in Drosophila imaginal discs / Jiajie Xu, Ting Su, Sherzod A. Tokamov, and Richard G. Fehon -- Localization of hippo signaling components in Drosophila by fluorescence and immunofluorescence / Cordelia Rauskolb and Kenneth D. Irvine -- Bimolecular fluorescence complementation (BiFC) in tissue culture and in developing tissues of Drosophila to study protein-protein interactions / Yurika Matsui and Zhi-Chun Lai -- Immunohistochemistry to study YAP in human tissue samples / Franziska Haderk, Victor Olivas, and Trever G. Bivona -- Immunofluorescence study of endogenous YAP in mammalian cells / Valentina Rausch and Carsten G. Hansen -- Immunofluorescence microscopy to study endogenous TAZ in mammalian cells / Nathan M. Kingston, Andrew M. Tilston-Lunel, Julia Hicks-Berthet, and Xaralabos Varelas -- Nuclear/cytoplasmic fractionation to study hippo effectors / Maria Chatzifrangkeskou and Eric O'Neill -- Luciferase reporter assays to determine YAP/TAZ activity in mammalian cells / Sirio Dupont -- Quantitative real-time PCR to measure YAP/TAZ activity in human cells / Xiaolei Cao and Bin Zhao -- HTRFʼ total and phospho-YAP (Ser127) cellular assays / Diana Zindel, Claire Vol, Odile Lecha, Isabelle Bequignon, Merve Bilgic, Marion Vereecke, Fabienne Charrier-Savournin, Mał·teþ Romier, Eric Trinquet, Jean-Philippe Pin, Julie Pannequin, Thomas Roux, Elodie Dupuis, and Laurent Preþzeau -- Studying YAP-mediated 3D morphogenesis using fish embryos and human spheroids / Yoichi Asaoka, Hitoshi Morita, Hiroko Furumoto, Carl-Philipp Heisenberg, and Makoto Furutani-Seiki -- Regulation of YAP/TAZ activity by mechanical cues : an experimental overview / Sirio Dupont -- CRISPR-mediated approaches to regulate YAP/TAZ levels / Ryan J. Quinton and Neil J. Ganem -- Hippo pathway regulation by tyrosine kinases / Nina Reuven, Matan Shanzer, and Yosef Shaul -- Structural and biochemical analyses of the core components of the hippo / Lisheng Ni and Xuelian Luo -- Isothermal titration calorimetry assays to measure binding affinities in vitro / Kui Lin and Geng Wu -- GST pull-down assay to measure complex formations / Sun-Yong Kim and Toshio Hakoshima -- Determining the phosphorylation status of hippo components YAP and TAZ using Phos-tag / Rui Chen, Steven W. Plouffe, and Kun-Liang Guan -- Quantifying the kinase activities of MST1/2 / Niamh A. O'Driscoll and David Matallanas -- Measuring the kinase activities of the LATS/NDR protein kinases / Axander Hergovich -- MST1/2 kinase assays using recombinant proteins / Marta Gomez, Yavuz Kulaberoglu, and Alexander Hergovich -- Visualizing HIPPO signaling components in mouse early embryonic development / Tristan Frum and Amy Ralston -- Hippo signaling pathway in regenerative medicine / Lixin Hong, Yuxi Li, Qingxu Liu, Qinghua Chen, Lanfen Chen, and Dawang Zhou.

This detailed book covers numerous expert methodologies to examine Hippo signaling on the structural, molecular, cellular, and organismal level. Beginning with a section on Drosophila genetics, the volume continues with parts exploring molecular and cell biological studies of the Hippo pathway, the use of structural biology and biochemistry, and a brief look at the Hippo pathway in mouse models. Written for the highly successful Methods in Molecular Biology series, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.