Molecular cardiology : methods and protocols / edited by Zhongjie Sun.

Includes bibliographical references and index.

Introduction to methods in molecular cardiology / Zhongjie Sun -- Gene therapy approaches to cardiovascular disease / J. Mark Jones and Walter J. Koch -- Gene therapy for myocardial ischemia using hypoxia inducible double plasmid / Yi Tang -- Cardiac gene delivery using dna-adenoviral constructs / J. Mark Jones and Walter J. Koch -- Manipulation of neuronal nitric oxide synthase within the paraventricular nucleus using adenovirus and antisense technology / Yi-Fan Li [and others] -- TAT-mediated protein transduction: delivering biologically active proteins into the heart / Asa B. Gustafson, Roberta A. Gottlieb, and David J. Granville -- Expression of human eNOS in cardiac and endothelial cells / Xiuqing Wang and Zhongjie Sun -- Strategies of conditional gene expression in myocardium: an overview / Heather Heine [and others] -- Gene silencing method using adenoviral RNAi vector -- In cardiomyocytes and vascular smooth muscule cells / Hideko Kasahara and Hiroki Aoki -- Cardiac commitment of embryonic cells for myocardial repair / Dana Zeineddine [and others] -- Autologous mesenchymal stem cells for post ischemic myocardial repair / Yao Liang Tang -- Cellular therapy with autologous skeletal myoblasts for ischemic heart disease and heart failure / Yao Liang Tang -- Protocols for myocardial infarction repair using fetal cardiac myocytes / Sharon Etzion [and others] -- Methods for examining stem cells in post ischemic and transplanted hearts / Nana Rezai [and others] -- Endothelial progenitor cell/ex vivo expansion and gene transfer / Hideki Iwaguro and Takayuki Asahara -- Delineation of sequences essential for specific promoter activation during pressure overloaded hypertrophy or factor-induced hypertrophy / Chellam Rajamanickam and Radhakrishnan Jeejabai -- Evaluation of cardiac isoform of 2 macroglobulin as a factor inducing cardiac hypertrophy / Chellam Rajamanickam and Radhakrishnan Jeejabai -- Detection of apoptosis in cardiovascular diseases / Arwen Hunter, Jonathan C. Choy, and David J. Granville -- Detection of cardiac signaling in the injured and hypertrophied heart / Xiaoning Si [and others] -- Affymetrix oligonucleotide analysis of gene expression in the injured heart / Bobby Yanagawa [and others] -- Identification and validation of loci and candidate genes -- Linked to cardiac hypertrophy / Bastien Llamas and Christian F. Deschepper -- Induction and analysis of cardiac hypertrophy in transgenic animal models / Marcos E. Barbosa, Natalia Alenina, and Michael Bader -- Methods for the detection of altered -adrenergic signaling pathways in hypertrophied hearts / Matthew J. Wolf, Hideo Takibana, and Howard A. Rockman -- Transgenic mouse models for cardiac dysfunction by a specific genetic manipulation / Gopal J. Babu and Muthu Periasamy -- Cardiomyocyte defects in diabetic models and protection with cardiac-targeted transgenes / Xia Shen [and others] -- Protocols for cardiac-specific overexpression of angiotensin II type 1 receptor in transgenic rats / Sigrid Hoffmann.

Print version record.

The application of molecular technology to the heart opens a new and promising avenue for both the study and cure of heart diseases that were once thought to be incurable. In Molecular Cardiology: Methods and Protocols, leading cardiac researchers from around the world describe in detail their own proven, cutting-edge techniques for gene therapy and stem cell therapy for heart diseases. The authors provide readily reproducible methods for genetic dissection, signal transduction, and microarray analysis. The protocols include techniques for gene transfer to heart tissue using viral and nonviral vectors, the basis for gene therapy of heart diseases, and for producing transgenic models of heart diseases, the novel and powerful tools for the study of heart disease pathogenesis. Many of the protocols employ not only molecular and cell biology techniques, but also in vivo approaches to myocardial repair using different types of stem cells, and include methods for expansion, purification, and genetic modification of stem cells. The protocols offer step-by-step laboratory instructions, an introduction outlining the principle behind the technique, and tips on troubleshooting and avoiding known pitfalls. Authoritative and highly practical, Molecular Cardiology: Methods and Protocols offers basic and clinical researchers the first major collection of the state-of-the-art molecular and cell biology techniques for the study and treatment of genetic and acquired heart diseases.

English.