| 000 | 07210cam a22008534a 4500 | ||
|---|---|---|---|
| 001 | ocn209948182 | ||
| 003 | OCoLC | ||
| 005 | 20250702164734.0 | ||
| 006 | m o d | ||
| 007 | cr |n||||||||| | ||
| 008 | 050913s2006 njua ob 001 0 eng c | ||
| 040 |
_aCOO _beng _epn _cCOO _dGW5XE _dCUV _dN$T _dYDXCP _dLGG _dPUL _dOCLCQ _dCASUM _dE7B _dIDEBK _dOCLCQ _dUBY _dOCLCA _dOCLCF _dVVL _dOCLCQ _dDEBSZ _dOCLCQ _dYNG _dCSU _dOCLCQ _dEUW _dOCLCQ _dVT2 _dOCLCA _dOTZ _dOCLCQ _dOCLCA _dBUF _dOCLCO _dSTF _dMERER _dOCLCQ _dU3W _dOCLCO _dOCLCQ _dAU@ _dOCLCO _dOCLCQ _dYOU _dLND _dOCLCO _dCNTRU _dOCLCQ _dOCLCO _dOCLCA _dHS0 _dUWK _dSXB _dMM9 _dINARC _dLVT _dOCLCQ _dOCLCO _dOCLCQ _dOCLCO _dWSU _dOCLCO _dOCLCL _dUKAHL _dOCLCQ |
||
| 016 | 7 |
_z101257073 _2DNLM |
|
| 019 |
_a127170480 _a173611173 _a234238280 _a316271701 _a642461379 _a648278058 _a771205398 _a964903100 _a967615305 _a1005784826 _a1035717777 _a1043065958 _a1077868928 _a1081273262 _a1087306348 _a1097275110 _a1148094504 _a1164928789 _a1166171194 _a1171450752 _a1173364076 _a1176180896 _a1229765176 |
||
| 020 |
_a1588295761 _q(alk. paper) |
||
| 020 |
_a1597450952 _q(e-book) |
||
| 020 |
_a9781597450959 _q(e-book) |
||
| 020 | _a9781588295767 | ||
| 020 | _a1280832169 | ||
| 020 | _a9781280832161 | ||
| 020 | _a9786610832163 | ||
| 020 | _a6610832161 | ||
| 024 | 7 |
_a10.1385/1597450952. _2doi |
|
| 029 | 1 |
_aAU@ _b000056659772 |
|
| 029 | 1 |
_aDEBSZ _b423049860 |
|
| 029 | 1 |
_aNZ1 _b13072909 |
|
| 035 |
_a(OCoLC)209948182 _z(OCoLC)127170480 _z(OCoLC)173611173 _z(OCoLC)234238280 _z(OCoLC)316271701 _z(OCoLC)642461379 _z(OCoLC)648278058 _z(OCoLC)771205398 _z(OCoLC)964903100 _z(OCoLC)967615305 _z(OCoLC)1005784826 _z(OCoLC)1035717777 _z(OCoLC)1043065958 _z(OCoLC)1077868928 _z(OCoLC)1081273262 _z(OCoLC)1087306348 _z(OCoLC)1097275110 _z(OCoLC)1148094504 _z(OCoLC)1164928789 _z(OCoLC)1166171194 _z(OCoLC)1171450752 _z(OCoLC)1173364076 _z(OCoLC)1176180896 _z(OCoLC)1229765176 |
||
| 037 |
_a978-1-58829-576-7 _bSpringer _nhttp://www.springerlink.com |
||
| 042 | _apcc | ||
| 050 | 4 |
_aQH603.I54 _bI59 2006 |
|
| 060 | 4 |
_aW1 _bME9616J v.337 2006 |
|
| 060 | 4 |
_aQU 55 _bI69 2006 |
|
| 070 |
_aQH506 _b.M45 v. 337 |
||
| 072 | 7 |
_aSCI _x017000 _2bisacsh |
|
| 072 | 7 |
_aPSB. _2bicssc |
|
| 082 | 0 | 4 |
_a571.6/4 _222 |
| 049 | _aMAIN | ||
| 245 | 0 | 0 |
_aIon channels : _bmethods and protocols / _cedited by James D. Stockand, Mark S. Shapiro. |
| 260 |
_aTotowa, N.J. : _bHumana Press, _c©2006. |
||
| 300 |
_a1 online resource (xii, 212 pages) : _billustrations (some color) |
||
| 336 |
_atext _btxt _2rdacontent |
||
| 337 |
_acomputer _bc _2rdamedia |
||
| 338 |
_aonline resource _bcr _2rdacarrier |
||
| 490 | 1 |
_aMethods in molecular biology, _x1064-3745 ; _v337 |
|
| 504 | _aIncludes bibliographical references and index. | ||
| 505 | 0 | _aFunctional reconstitution of the human epithelial Na+ channel in a mammalian expression system / Alexander Staruschenko [and others] -- Overexpression of proteins in neurons using replication-deficient virus / Richard M. Ahlquist and Jane M. Sullivan -- Exogenous expression of proteins in neurons using the biolistic particle delivery system / Nikita Gamper and Mark S. Shapiro. | |
| 505 | 0 | _aTertiary and quaternary structure formation of voltage-gated potassium channels / John M. Robinson, Andrey Kosolapov, and Carol Deutsch -- Biophysical approach to determine the subunit stoichiometry of the epithelial sodium channel using the xenopus laevis oocyte expression system / Farhad Kosari, Shaohu Sheng, and Thomas R. Kleyman -- Spectroscopy-based quantitative fluorescence resonance energy transfer analysis / Jie Zheng -- Probing the effects of phosphoinositides on ion channels / Chou-Long Huang -- Epithelial sodium channel in planar lipid bilayers / Bakhrom K. Berdiev and Dale J. Benos. | |
| 505 | 0 | _aA simple in vivo method for assessing changes of membrane-bound ion channel density in xenopus oocytes / Mouhamed S. Awayda [and others] -- Preparation of cortical brain slices for electrophysiological recording / Costa M. Colbert -- Juxtacellular labeling and chemical phenotyping of extracellulary recorded neurons in vivo / Glenn M. Toney and Lynette C. Daws -- Carbon fiber amperometry in the study of ion channels and secretion / Duk-Su Koh -- Genetic screening for functionality of bacterial potassium channel mutants using K+ update-deficient escherichia coli / Lyubov V. Parfenova and Brad S. Rothberg -- KCNQ1 K+ channel-mediated cardiac channelopathies / Gildas Loussouarn, Isabelle Baró, and Denis Escande -- Tissue-specific transgenic and knockout mice / Andrée Porret [and others]. | |
| 588 | 0 | _aPrint version record. | |
| 520 | _aIt is now clear that ion channels play essential roles in cell biology and physiology and that their dysfunction is the root cause of many human diseases. In Ion Channels: Methods and Protocols, internationally recognized biomedical scientists describe recent technological breakthroughs and demonstrate their use in successful experimental designs. The diverse applications range from the study of allosteric regulation of ion channel activity using a classic mutagenesis approach, to the study of channel subunit stoichiometry using a novel biophysical approach based on fluorescence resonance energy transfer. Highlights include methods for heterologous expression of ion channels in cells, for determining channel structure-function, for studying channel regulation and physiological function, and for genetic screening and investigating channelopathies. The protocols follow the successful Methods in Molecular Biology series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and highly practical, Ion Channels: Methods and Protocols offer both novice and experienced researchers a versatile collection of proven techniques for illuminating the structure, function, and regulation of ion channel proteins and finding suitable drug targets when they cause disease. | ||
| 546 | _aEnglish. | ||
| 650 | 0 |
_aIon channels. _982312 |
|
| 650 | 2 |
_aIon Channels _982312 |
|
| 650 | 6 |
_aCanaux ioniques. _9101401 |
|
| 650 | 7 |
_aSCIENCE _xLife Sciences _xCell Biology. _2bisacsh _980168 |
|
| 650 | 7 |
_aIon channels _2fast _982312 |
|
| 655 | 2 | _aLaboratory Manual | |
| 655 | 4 |
_aInternet Resources. _980513 |
|
| 655 | 4 |
_aIllustrations. _9974539 |
|
| 655 | 7 |
_aLaboratory manuals. _2lcgft |
|
| 655 | 7 |
_aManuels de laboratoire. _2rvmgf _9966128 |
|
| 700 | 1 |
_aStockand, James D. _984233 |
|
| 700 | 1 |
_aShapiro, Mark S. _984234 |
|
| 776 | 0 | 8 |
_iPrint version: _tIon channels. _dTotowa, N.J. : Humana Press, ©2006 _z1588295761 _w(DLC) 2005026667 _w(OCoLC)61518303 |
| 830 | 0 |
_aMethods in molecular biology (Clifton, N.J.) ; _vv. 337. |
|
| 856 | 4 | 0 | _uhttps://link-springer-com.libraryproxy.ist.ac.at/10.1385/1597450952 |
| 938 |
_aAskews and Holts Library Services _bASKH _nAH40390396 |
||
| 938 |
_aebrary _bEBRY _nebr10182079 |
||
| 938 |
_aEBSCOhost _bEBSC _n201523 |
||
| 938 |
_aProQuest MyiLibrary Digital eBook Collection _bIDEB _n83216 |
||
| 938 |
_aInternet Archive _bINAR _nionchannelsmetho0337unse |
||
| 938 |
_aYBP Library Services _bYANK _n2712369 |
||
| 994 |
_a92 _bATIST |
||
| 999 |
_c651968 _d651968 |
||