000 06157cam a2200697 a 4500
001 ocn700048824
003 OCoLC
005 20250702165333.0
006 m o d
007 cr cn|||||||||
008 110202s2011 nyua ob 001 0 eng d
040 _aGW5XE
_beng
_epn
_cGW5XE
_dOCLCQ
_dUKMGB
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016 7 _a015761426
_2Uk
019 _a771205315
_a1043061842
_a1071908676
_a1087357522
020 _a9781607619505
_q(electronic bk.)
020 _a1607619504
_q(electronic bk.)
020 _a9781607619499
020 _a1607619490
029 1 _aNZ1
_b15212475
035 _a(OCoLC)700048824
_z(OCoLC)771205315
_z(OCoLC)1043061842
_z(OCoLC)1071908676
_z(OCoLC)1087357522
037 _a978-1-60761-949-9
_bSpringer
_nhttp://www.springerlink.com
050 4 _aQH211
_b.L54 2011
082 0 4 _a570.28/2
_222
049 _aMAIN
245 0 0 _aLight microscopy :
_bmethods and protocols /
_cedited by Hélio Chiarini-Garcia, Rossana C.N. Melo.
260 _aNew York :
_bSpringer,
_c©2011.
300 _a1 online resource (x, 244 pages) :
_billustrations (some color)
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
490 1 _aMethods in molecular biology ;
_v689
490 1 _aSpringer protocols
505 0 0 _tGlycol methacrylate embedding for improved morphological, morphometrical, and immunohistochemical investigations under light microscopy: testes as a model /
_rHélio Chiarini-Garcia, Gleydes Gambogi Parreira, and Fernanda R.C.L. Almeida --
_tHistological processing of teeth and periodontal tissues for light microscopy analysis /
_rGerluza Aparecida Borges Silva, Adriana Moreira, and José Bento Alves --
_tLarge plant samples: how to process for GMA embedding /
_rÉlder Antônio Sousa Paiva, Sheila Zambello de Pinho, and Denise Maria Trombert Oliveira --
_tImage cytometry: nuclear and chromosomal DNA quantification /
_rCarlos Roberto Carvalho, Wellington Ronildo Clarindo, and Isabella Santiago Abreu --
_tHistological approaches to study tissue parasitism during the experimental Trypanosoma cruzi infection /
_rDaniela L. Fabrino, Grazielle A. Ribeiro, Livia Teixeira, and Rossana C.N. Melo --
_tIntravital microscopy to study leukocyte recruitment in vivo /
_rVanessa Pinho [and others] --
_tIntroduction to fluorescence microscopy /
_rIonita C. Ghiran --
_tUsing the fluorescent styryl dye FM1-43 to visualize synaptic vesicles exocytosis and endocytosis in motor nerve terminals /
_rErnani Amaral, Silvia Guatimosim, and Cristina Guatimosim --
_tImaging lipid bodies within leukocytes with different light microscopy techniques /
_rRossana C.N. Melo [and others] --
_tEicosa cell: an immunofluorescent-based assay to localize newly synthesized eicosanoid lipid mediators at intracellular sites /
_rChristianne Bandeira-Melo, Peter F. Weller, and Patricia T. Bozza --
_tNestin-driven green fluorescent protein as an imaging marker for nascent blood vessels in mouse models of cancer /
_rRobert M. Hoffman --
_tImaging calcium sparks in cardiac myocytes /
_rSilvia Guatimosim, Cristina Guatimosim, and Long-Sheng Song --
_tLight microscopy in aquatic ecology: methods for plankton communities studies /
_rMaria Carolina S. Soares [and others] --
_tFluorescence immunohistochemistry in combination with differential interference contrast microscopy for studies of semi-ultrathin specimens of epoxy resin-embedded samples /
_rShin-ichi Iwasaki and Hidekazu Aoyagi.
504 _aIncludes bibliographical references and index.
588 0 _aPrint version record.
520 _aOf all scientific instruments, probably none has had more applications in the life sciences than the light microscope. In Light Microscopy: Methods and Protocols, expert researchers explore the basics and the latest advances in microscope instrumentation, sample preparation, and imaging techniques, all of which have been producing fundamental insights into the functions of cells and tissues. Chapters cover a variety of bright field and fluorescence microscopy-based approaches that are central to the study of a range of biological questions, providing information on how to prepare cells and tissues for microscopic investigations, covering detailed staining procedures, and exploring methods to analyze images and interpret the results accurately. Composed in the highly successful Methods in Molecular Biology"!series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls. Comprehensive and current, Light Microscopy: Methods and Protocols is an essential handbook for all researchers who are exploring the intriguing microscopic world of the cell
650 0 _aMicroscopy.
_95754
650 2 _aMicroscopy
_xmethods
_96260
650 2 _aMicroscopy, Fluorescence
_xmethods
_986966
650 2 _aMicroscopy
_95754
650 6 _aMicroscopie.
_985299
650 7 _amicroscopy.
_2aat
_95754
650 7 _aMicroscopy
_2fast
_95754
653 0 0 _amicroscopie
653 0 0 _amicroscopy
653 0 0 _abiologische technieken
653 0 0 _abiological techniques
653 1 0 _aBiological Techniques
653 1 0 _aBiologische technieken
655 2 _aLaboratory Manual
655 7 _aLaboratory manuals.
_2lcgft
655 7 _aManuels de laboratoire.
_2rvmgf
_9966128
700 1 _aChiarini-Garcia, Hélio.
_986967
700 1 _aMelo, Rossana C.
_986968
758 _ihas work:
_aLight Microscopy (Text)
_1https://id.oclc.org/worldcat/entity/E39PCFrBCk3cx3fGXGVRt9p4G3
_4https://id.oclc.org/worldcat/ontology/hasWork
776 0 8 _iPrint version:
_tLight microscopy.
_dNew York : Springer, ©2011
_z9781607619499
_w(OCoLC)648933191
830 0 _aMethods in molecular biology (Clifton, N.J.) ;
_vv. 689.
830 0 _aSpringer protocols (Series)
_980328
856 4 0 _uhttps://link-springer-com.libraryproxy.ist.ac.at/10.1007/978-1-60761-950-5
994 _a92
_bATIST
999 _c652437
_d652437