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020 _a1617799807
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020 _a1617799793
020 _a9781617799792
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024 7 _a10.1007/978-1-61779-980-8
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029 1 _aNZ1
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035 _a(OCoLC)809055932
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050 4 _aQH588.S83
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060 0 0 _aW1
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082 0 4 _a616.02/774
_223
049 _aMAIN
245 0 0 _aProgenitor cells :
_bmethods and protocols /
_cedited by Kimberly A. Mace, Kristin M. Braun.
260 _aNew York :
_bHumana Press,
_c©2012.
300 _a1 online resource (xiv, 408 pages) :
_billustrations (some color)
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
347 _atext file
_bPDF
_2rda
490 1 _aMethods in molecular biology,
_x1940-6029 ;
_v916
504 _aIncludes bibliographical references and index.
505 0 0 _tIsolation of satellite cells from single muscle fibers from young, aged, or dystrophic muscles /
_rValentina Di Foggia and Lesley Robson --
_tTerminal differentiation of human epidermal stem cells on micro-patterned substrates /
_rJohn T. Connelly --
_tIsolation, culture, and potentiality assessment of lung alveolar stem cells /
_rFeride Oeztuerk-Winder and Juan-Jose Ventura --
_tThree-dimensional in vitro culture techniques for mesenchymal stem cells /
_rFatima A. Saleh, Jessica E. Frith, Jennifer A. Lee, and Paul G. Genever --
_tIsolation of adult stem cells and their differentiation to schwann cells /
_rCristina Mantovani, Giorgio Terenghi, and Susan G. Shawcross --
_tFunctional purification of human and mouse mammary stem cells /
_rDaniela Tosoni, Pier Paolo Di Fiore, and Salvatore Pece --
_tIsolation and expansion of endothelial progenitor cells derived from mouse embryonic stem cells /
_rS. Bahram Bahrami, Mandana Veiseh, and Nancy J. Boudreau --
_tTranscriptome analysis of Drosophila neural stem cells /
_rKatrina S. Gold and Andrea H. Brand --
_tLive imaging for studying asymmetric cell division in the C. elegans embryo /
_rAlexia Rabilotta, Rana Amini, and Jean-Claude Labbe --
_tTol2-mediated gene transfer and in ovo electroporation of the otic placode : a powerful and versatile approach for investigating embryonic development and regeneration of the chicken inner ear /
_rStephen Freeman, Elena Chrysostomou, Koichi Kawakami, Yoshiko Takahashi, and Nicolas Daudet --
_tLabeling primitive myeloid progenitor cells in Xenopus /
_rRicardo Costa, Yaoyao Chen, Roberto Paredes, and Enrique Amaya --
_tIdentification of oocyte progenitor cells in the zebrafish ovary /
_rBruce W. Draper --
_tFACS analysis of the planarian stem cell compartment as a tool to understand regenerative mechanisms/
_rBelen Tejada Romero, Deborah J. Evans, and A. Aziz Aboobaker --
_tClonal and lineage analysis of melanocyte stem cells and their progeny in the zebrafish /
_rRobert C. Tryon and Stephen L. Johnson --
_tReconstitution of the central nervous system during salamander tail regeneration from the implanted neurospheres /
_rLevan Mchedlishvili, Vladimir Mazurov, and Elly M. Tanaka --
_tFollowing the fate of neural progenitors by homotopic/homochronic grafts in Xenopus embryos /
_rRaphael Thuret and Nancy Papalopulu --
_tAnalyzing the angiogenic potential of Gr-1+CD11b+ immature myeloid cells from murine wounds /
_rElahe Mahdipour and Kimberly A. Mace --
_tIn vivo imaging of hematopoietic stem cells in the bone marrow niche /
_rOliver Barrett, Roberta Sottocornola, and Cristina Lo Celso --
_tCharacterizing the phenotype of murine epidermal progenitor cells : complementary whole-mount visualization and flow cytometry strategies /
_rKorinna D. Henseleit, Ann P. Wheeler, Gary Warnes, and Kristin M. Braun --
_tMurine aggregation chimeras and wholemount imaging in airway stem cell biology /
_rIan R. Rosewell and Adam Giangreco --
_tLive imaging of primitive endoderm precursors in the mouse blastocyst /
_rJoanna B. Grabarek and Berenika Plusa --
_tUtilizing DNA mutations to trace epithelial cell lineages in human tissues /
_rSebastian Zeki, Trevor A. Graham, and Stuart A.C. McDonald --
_tNF-Ya protein delivery as a tool for hematopoietic progenitor cell expansion /
_rAlevtina D. Domashenko, Susan Wiener, and Stephen G. Emerson --
_tExploring the link between human embryonic stem cell organization and fate using tension-calibrated extracellular matrix functionalized polyacrylamide gels /
_rJohnathon N. Lakins, Andrew R. Chin, and Valerie M. Weaver --
_tIsolation of circulating angiogenic cells /
_rErin E. Vaughan and Timothy O'Brien --
_tMethods for characterization/manipulation of human corneal stem cells and their applications in regenerative medicine /
_rFrancesca Corradini, Beatrice Venturi, Graziella Pellegrini, and Michele De Luca --
_tALDH as a marker for enriching tumorigenic human colonic stem cells /
_rAnitha Shenoy, Elizabeth Butterworth, and Emina H. Huang --
_tProtocols for investigating microRNA functions in human neural progenitor cells /
_rSandra Almeida, Celine Delaloy, Lei Liu, and Fen-Biao Gao.
520 _aProgenitor cells have become important in regenerative medicine therapies, due to their potential to differentiate into many cell types. This capability, and understanding how to regulate the cells, will provide the basis for future cell therapies aimed at correcting tissue and organ dysfunction as a result of disease or injury. In, Progenitor Cells: Methods and Protocols, expert researchers in the field detail many of the methods which are now commonly used to investigate progenitor cells. These include methods and techniques of the manipulation of physical forces that shape progenitor cell behavior, studying progenitor cells in vivo, using non-mammalian and mammalian model systems, and investigating human progenitor cells, including their isolation, characterization and application in cell-based therapies. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Progenitor Cells : Methods and Protocols seeks to aid scientists in the further study progenitor cells and how they are studied across multiple systems.
546 _aEnglish.
650 0 _aStem cells
_vLaboratory manuals.
_980498
650 0 _aStem cells.
_980437
650 1 2 _aStem Cells
_980437
650 2 _aLaboratory Manuals
_981487
650 6 _aCellules souches
_vManuels de laboratoire.
_9970157
650 6 _aCellules souches.
_987231
650 7 _aStem cells
_2fast
_980437
653 4 _aLife sciences.
653 4 _aCytology.
653 4 _aStem cells.
653 4 _aCell Biology.
653 0 0 _acellen
653 0 0 _acells
653 0 0 _acelbiologie
653 0 0 _acellular biology
653 0 0 _astamcellen
653 1 0 _aCellular Biology
653 1 0 _aCelbiologie
655 2 _aLaboratory Manual
655 7 _aLaboratory manuals
_2fast
655 7 _aLaboratory manuals.
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655 7 _aManuels de laboratoire.
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_9966128
700 1 _aMace, Kimberly A.
_q(Kimberly Ann),
_d1968-
_4edt
_1https://id.oclc.org/worldcat/entity/E39PCjxVCJgXpppWBpqrd7gWcK
_988230
700 1 _aBraun, Kristin M.
_4edt
_988231
758 _ihas work:
_aProgenitor cells (Text)
_1https://id.oclc.org/worldcat/entity/E39PCGYMQRXrTcWFTKbGFt6qPP
_4https://id.oclc.org/worldcat/ontology/hasWork
776 0 8 _iPrint version:
_tProgenitor cells.
_dNew York : Humana Press, ©2012
_w(DLC) 2012943274
830 0 _aMethods in molecular biology (Clifton, N.J.) ;
_v916.
_x1064-3745
856 4 0 _uhttps://link-springer-com.libraryproxy.ist.ac.at/10.1007/978-1-61779-980-8
938 _aAskews and Holts Library Services
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938 _aYBP Library Services
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